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Volume 64, Issue Pt_9

gen. nov., sp. nov., a phosphate-solubilizing and nitrogen-fixing alphaproteobacterium isolated from the rhizosphere of a natural salt-meadow plant Free

Abstract

A phosphate-mobilizing, Gram-negative bacterium was isolated from rhizospheric soil of from a natural salt meadow as part of an investigation of rhizospheric bacteria from salt-resistant plant species and evaluation of their plant-growth-promoting abilities. Cells were rods, motile, strictly aerobic, oxidase-positive and catalase-negative. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain E19 was distinct from other taxa within the class . Strain E19 showed less than 93.5 % 16S rRNA gene sequence similarity with members of the genera (≤93.5 %), (≤93.1 %), (≤93.1 %), (≤93.1 %) and (≤93.0 %) and was most closely related to (93.5 % 16S rRNA gene sequence similarity to the type strain). The sole respiratory quinone was Q-10, and the polar lipids comprised phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an aminolipid and an unidentified phospholipid. Major fatty acids were Cω7 (71.4 %), summed feature 2 (C 3-OH and/or iso-C; 8.3 %), C (7.9 %) and C (6.1 %). The DNA G+C content of strain E19 was 59.9±0.7 mol%. The capacity for nitrogen fixation was confirmed by the presence of the gene and the acetylene reduction assay. On the basis of the results of our polyphasic taxonomic study, the new isolate represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is E19 ( = LMG 27460 = KACC 17263).

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2014-09-01
2024-04-27
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Hartmannibacter diazotrophicus gen. nov., sp. nov., a phosphate-solubilizing and nitrogen-fixing alphaproteobacterium isolated from the rhizosphere of a natural salt-meadow plant
Int J Syst Evol Microbiol 64, 3160 (2014); https://doi.org/10.1099/ijs.0.064154-0
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