Full text loading...
Abstract
The taxonomic status of a rhizospheric soil actinomycete, designated R8-39T, was established using a polyphasic approach. The organism had phenotypic and morphological characteristics consistent with its classification in the genus Allokutzneria . Phylogenetic analysis based on an almost complete 16S rRNA gene sequence showed that the strain formed a monophyletic clade with the type strains of members of the genus Allokutzneria . Strain R8-39T displayed the highest levels of 16S rRNA gene sequence similarity to Allokutzneria albata DSM 44149T (98.8 %) and Allokutzneria multivorans YIM 120521T (98.3 %). However, the DNA–DNA hybridization values between strain R8-39T and A. albata and A. multivorans were clearly below the 70 % threshold. The organism was found to have chemical characteristics consistent with its classification in the genus Allokutzneria . Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose, galactose, glucose, mannose, rhamnose and ribose. The main menaquinone was MK-9(H4). No mycolic acid was detected. The G+C content of the genomic DNA was 71.8 mol%. In addition, strain R8-39T had a phenotypic profile that readily distinguished it from recognized representatives of the genus Allokutzneria . It is evident from the combined genotypic and phenotypic properties that strain R8-39T represents a novel species of the genus Allokutzneria . The proposed name for this species is Allokutzneria oryzae sp. nov.; the type strain is R8-39T ( = BCC 60399T = NBRC 109649T).
- Published Online:
Funding
- Kasetsart University Research and Development Institute (KURDI)
- Thailand, Office of the Higher Education Commission
- Kitasato Institute for Life Sciences, Kitasato University
- Faculty of Science, Kasetsart University
- Kasetsart University