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- Volume 65, Issue 10
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f Spirosoma fluviale sp. nov., isolated from river water
- Authors: Kouta Hatayama1 , Teruaki Kuno2
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- VIEW AFFILIATIONS
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1 1Sagami Chemical Research Institute, Ayase, Kanagawa 252-1193, Japan 2 2Department of Chemistry, School of Science, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan
- Correspondence Kouta Hatayama [email protected]
- First Published Online: 01 October 2015, International Journal of Systematic and Evolutionary Microbiology 65: 3447-3450, doi: 10.1099/ijsem.0.000433
- Subject: NEW TAXA - Bacteroidetes
- Cover date:




Spirosoma fluviale sp. nov., isolated from river water, Page 1 of 1
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A bacterial strain, designated MSd3T, was isolated from a freshwater sample collected from the Hosoda River in Japan. The cells of strain MSd3T were Gram-stain-negative, non-spore-forming, aerobic, non-motile, curved rods forming rings, coils and undulating filaments. The 16S rRNA gene sequence of strain MSd3T showed closest similarity to that of Spirosoma linguale DSM 74T (97.6 % similarity) and similarity to other members of the genus Spirosoma ranged from 90.3 to 95.9 %. Strain MSd3T contained menaquinone 7 as the sole respiratory quinone. The major cellular fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and C16 : 1ω5c. The polar lipids were phosphatidylethanolamine, three unidentified aminophospholipids and three unidentified polar lipids. The DNA G+C content was 53.3 mol%. The DNA–DNA relatedness between strain MSd3T and S. linguale DSM 74T was 19 % or 25 % (reciprocal value). From the chemotaxonomic and physiological data and the levels of DNA–DNA relatedness, strain MSd3T should be classified as the representative of a novel species of the genus Spirosoma, for which the name Spirosoma fluviale sp. nov. (type strain MSd3T = JCM 30659T = DSM 29961T) is proposed.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Spirosoma fluviale MSd3T is LC019141.
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One supplementary table and one supplementary figure are available with the online Supplementary Material.
© 2015 IUMS | Published by the Microbiology Society
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