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- Volume 66, Issue 1
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f Bradyrhizobium vignae sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of Vigna and Arachis
- Authors: Jann Lasse Grönemeyer1 , Thomas Hurek1 , Wiebke Bünger1 , Barbara Reinhold-Hurek1
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1 Department of Microbe-Plant Interactions, Center of Molecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, Postfach 33 04 40, D-28334, Bremen, Germany
- Correspondence Barbara Reinhold-Hurek [email protected]
- First Published Online: 01 January 2016, International Journal of Systematic and Evolutionary Microbiology 66: 62-69, doi: 10.1099/ijsem.0.000674
- Subject: NEW TAXA - Proteobacteria
- Cover date:




Bradyrhizobium vignae sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of Vigna and Arachis, Page 1 of 1
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Twenty one strains of symbiotic bacteria from root nodules of local races of cowpea (Vigna unguiculata), Bambara groundnut (Vigna subterranea) and peanuts (Arachis hypogaea) grown on subsistence farmers' fields in the Kavango region of Namibia, were previously characterized as a novel group within the genus Bradyrhizobium. To verify their taxonomic position, the strains were further analysed using a polyphasic approach. 16S rRNA gene sequences were most similar to Bradyrhizobium manausense BR 3351T, with Bradyrhizobium ganzhouense RITF806T being the most closely related type strain in the phylogenetic analysis, and Bradyrhizobium yuanmingense CCBAU 10071T in the ITS sequence analysis. Phylogenetic analysis of concatenated glnII-recA-rpoB-dnaK placed the strains in a highly supported lineage distinct from species of the genus Bradyrhizobium with validly published names; they were most closely related to Bradyrhizobium subterraneum 58 2-1T. The status of the species was validated by results of DNA–DNA hybridization. The combination of phenotypic characteristics from several tests, including carbon source utilization and antibiotic resistance, could be used to differentiate representative strains of species of the genus Bradyrhizobium with validly published names. Novel strain 7-2T induced effective nodules on Vigna subterranea, Vigna unguiculata, Arachis hypogaea and on Lablab purpureus. The DNA G+C content of strain 7-2T was 65.4 mol% (Tm). Based on the data presented, we conclude that these strains represent a novel species for which the name Bradyrhizobium vignae sp. nov. is proposed, with strain 7-2T [LMG 28791T, DSMZ 100297T, NTCCM0018T (Windhoek)] as the type strain.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 7-2T is KP899563. The GenBank/EMBL/DDBJ accession numbers for the ITS, glnII, recA, rpoB, dnaK, nifH and nodC sequences of strain 7-2T are KM378504, KM378443, KM378374, KM378308, KR259951, KM378251 and KT362339, respectively.
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Three supplementary figures and three supplementary tables are available with the online Supplementary Material.
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Abbreviations: ITS intergenic spacer MLSA multilocus sequence analysis.
© 2015 IUMS | Published by the Microbiology Society
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