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f sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of and

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sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of and , Page 1 of 1

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  • Twenty one strains of symbiotic bacteria from root nodules of local races of cowpea (), Bambara groundnut () and peanuts () grown on subsistence farmers' fields in the Kavango region of Namibia, were previously characterized as a novel group within the genus . To verify their taxonomic position, the strains were further analysed using a polyphasic approach. 16S rRNA gene sequences were most similar to BR 3351, with RITF806 being the most closely related type strain in the phylogenetic analysis, and CCBAU 10071 in the ITS sequence analysis. Phylogenetic analysis of concatenated placed the strains in a highly supported lineage distinct from species of the genus with validly published names; they were most closely related to 58 2-1. The status of the species was validated by results of DNA–DNA hybridization. The combination of phenotypic characteristics from several tests, including carbon source utilization and antibiotic resistance, could be used to differentiate representative strains of species of the genus with validly published names. Novel strain 7-2 induced effective nodules on and on The DNA G+C content of strain 7-2 was 65.4 mol% (Tm). Based on the data presented, we conclude that these strains represent a novel species for which the name sp. nov. is proposed, with strain 7-2 [LMG 28791, DSMZ 100297, NTCCM0018 (Windhoek)] as the type strain.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 7-2 is KP899563. The GenBank/EMBL/DDBJ accession numbers for the ITS, I, , , , and sequences of strain 7-2 are KM378504, KM378443, KM378374, KM378308, KR259951, KM378251 and KT362339, respectively.

  • Three supplementary figures and three supplementary tables are available with the online Supplementary Material.

  • Abbreviations:
    ITS intergenic spacer
    MLSA multilocus sequence analysis.

© 2015 IUMS | Published by the Microbiology Society

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2019-01-18
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