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f sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake

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  • Two extremely halophilic archaea, strains Cb34 and C170, belonging to the genus , were isolated from the brine of the hypersaline lake Aran-Bidgol in Iran. Cells of the two strains were motile, pleomorphic rods, stained Gram-variable and produced red-pigmented colonies. Strains Cb34 and C170 required 25 % (w/v) salts, pH 7.0 and 37 °C for optimal growth under aerobic conditions; 0.3 M Mg was required. Cells of both isolates were lysed in distilled water and hypotonic treatment with < 10 % NaCl provoked cell lysis. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that these two strains were closely related to B31 (98.8 %) and other members of the genus . In addition, studies based on the ′ gene revealed that strains Cb34 and C170 are placed among the species of and are closely related to B31, with ′ gene sequence similarity less than or equal to 95.7 %. The polar lipid patterns of both strains consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The DNA G+C content was 62.1–62.4 mol%. DNA–DNA hybridization studies confirmed that strains Cb34 and C170 constitute a distinct species. Data obtained in this study show that the two strains represent a novel species, for which the name sp. nov. is proposed. The type strain is Cb34 ( = CECT 8745 = IBRC-M 10233).

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and ′ gene sequences of strain Cb34 are HG421007 and KJ152360, respectively.

  • Two supplementary figures are available with the online Supplementary Material.

  • Abbreviations:
    DDH DNA–DNA hybridization
    PG phosphatidylglycerol
    PGP-Me phosphatidylglycerol phosphate methyl ester
    PGS phosphatidylglycerol sulfate
    S-DGD sulfated mannosyl glucosyl diether.

© 2015 IUMS | Published by the Microbiology Society

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