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- Volume 66, Issue 1
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f Actinoplanes lichenis sp. nov., isolated from lichen
- Authors: Wongsakorn Phongsopitanun1 , Atsuko Matsumoto2 , Yuki Inahashi2 , Takuji Kudo3 , Mihoko Mori2 , Kazuro Shiomi2 , Yoko Takahashi2 , Somboon Tanasupawat1
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1 1 Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 2 2 Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo, 108-8641, Japan 3 3 Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
- Correspondence Somboon Tanasupawat [email protected]
- First Published Online: 01 January 2016, International Journal of Systematic and Evolutionary Microbiology 66: 468-473, doi: 10.1099/ijsem.0.000746
- Subject: NEW TAXA - Actinobacteria
- Cover date:




Actinoplanes lichenis sp. nov., isolated from lichen, Page 1 of 1
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A novel species of the genus Actinoplanes, strain LDG1-22T, for which we propose the name Actinoplanes lichenis sp. nov., was isolated from a lichen sample collected from tree bark in Thailand. The taxonomic position of the species has been described based on a polyphasic approach. Strain LDG1-22T produced irregular sporangia on agar media. It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-9(H4); the polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and phosphatidylglycerol. Whole-cell hydrolysates contained ribose, glucose, mannose and small amounts of arabinose and xylose. The major cellular fatty acids were anteiso-C15 : 0 (31.2 %) and iso-C16 : 0 (14.2 %). Mycolic acids were absent. The G+C content was 73.6 %. 16S rRNA gene sequence analysis of strain LDG1-22T showed highest similarity (98.8 %) to Actinoplanes friuliensis DSM 45797T and it clustered with Actinoplanes nipponensis JCM 3264T and Actinoplanes missouriensis JCM 3121T in phylogenetic tree analysis. On the basis of the phenotypic characteristics and DNA–DNA relatedness, strain LDG1-22T could be distinguished from related species of the genus Actinoplanes and so represents a novel species of this genus. The type strain of Actinoplanes lichenis sp. nov. is LDG1-22T ( = JCM 30485T = TISTR 2343T = PCU 344T).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LDG1-22T is LC033899
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Three supplementary figures and one supplementary table are available with the online Supplementary Material.
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Abbreviations: LC-MS liquid chromatography-mass spectrometry.
© 2015 IUMS | Published by the Microbiology Society
-
Arai T. . ( 1975;). Culture Media for Actinomycetes Tokyo, Japan: The Society for Actinomycetes;.
-
Aretz W. , Meiwes J. , Seibert G. , Vobis G. , Wink J. . ( 2000;). Friulimicins: novel lipopeptide antibiotics with peptidoglycan synthesis inhibiting activity from Actinoplanes friuliensis sp. nov. I. Taxonomic studies of the producing microorganism and fermentation. J Antibiot (Tokyo) 53: 807––815 [CrossRef] [PubMed].
-
Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221––230 [CrossRef] [PubMed].
-
Coronelli C. , White R. J. , Lancini G. C. , Parenti F. . ( 1975;). Lipiarmycin, a new antibiotic from Actinoplanes. II. Isolation, chemical, biological and biochemical characterization. J Antibiot (Tokyo) 28: 253––259 [CrossRef] [PubMed].
-
Couch J. N. . ( 1950;). Actinoplanes, a new genus of the Actinomycetales . J Elisha Mitchell Sci Soc 66: 87––92.
-
Couch J. N. . ( 1963;). Some new genera and species of the Actinoplanaceae . J Elisha Mitchell Sci Soc 79: 53––70.
-
Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39: 224––229 [CrossRef].
-
Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368––376 [CrossRef] [PubMed].
-
Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783––791 [CrossRef].
-
Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum change for specific tree topology. Syst Zool 20: 406––416 [CrossRef].
-
Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H. N. . ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24: 54––63 [CrossRef].
-
Hayakawa M. , Nonomura H. . ( 1987;). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65: 501––509 [CrossRef].
-
Inahashi Y. , Matsumoto A. , Danbara H. , O¯mura S. , Takahashi Y. . ( 2010;). Phytohabitans suffuscus gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae isolated from plant roots. Int J Syst Evol Microbiol 60: 2652––2658 [CrossRef] [PubMed].
-
Kimura M. . ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111––120 [CrossRef] [PubMed].
-
Li B. , Xie C. , Yokota A. . ( 2007;). Nocardioides exalbidus sp. nov., a novel actinomycete isolated from lichen in Izu-Oshima island, Japan. Actinomycetologica 21: 22––26 [CrossRef].
-
Matsumoto A. , Takahashi Y. , Kudo T. , Seino A. , Iwai Y. , Omura S. . ( 2000;). Actinoplanes capillaceus sp. nov., a new species of the genus Actinoplanes . Antonie van Leeuwenhoek 78: 107––115 [CrossRef] [PubMed].
-
Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233––241 [CrossRef].
-
Parenti F. , Coronelli C. . ( 1979;). Members of the genus Actinoplanes and their antibiotics. Annu Rev Microbiol 33: 389––411 [CrossRef] [PubMed].
-
Parte A. C. . ( 2014;). LPSN—list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42: (D1), D613––D616 [CrossRef] [PubMed].
-
Saito H. , Miura K. I. . ( 1963;). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72: 619––629 [CrossRef] [PubMed].
-
Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406––425 [PubMed].
-
Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
-
Shirling E. B. , Gottlieb D. . ( 1966;). Method for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313––340 [CrossRef].
-
Somma S. , Gastaldo L. , Corti A. . ( 1984;). Teicoplanin, a new antibiotic from Actinoplanes teichomyceticus nov. sp. Antimicrob Agents Chemother 26: 917––923 [CrossRef] [PubMed].
-
Staneck J. L. , Roberts G. D. . ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28: 226––231 [PubMed].
-
Tamaoka J. , Komagata K. . ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25: 125––128 [CrossRef].
-
Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega 6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725––2729 [CrossRef] [PubMed].
-
Taylor H. D. , Knoche L. , Grauville W. C. . ( 1958;). Color Harmony Manual , 4th edn. Chicago: Container Corporation of America;.
-
Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673––4680 [CrossRef] [PubMed].
-
Tomiyasu I. . ( 1982;). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol 151: 828––837 [PubMed].
-
Uchida K. , Aida K. . ( 1984;). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30: 131––134.[CrossRef]
-
Vobis G. , Schäfer J. , Kämpfer P. . ( 2012;). Genus III. Actinoplanes Couch 1950, 89AL emend. Stackebrandt and Kroppenstedt 1987, 112. . In Bergey's Manual of Systematic Bacteriology , 2nd edn.., vol. 5, pp. 1058––1088. Edited by Goodfellow M. , Kämpfer P. , Busse H.-J. , Trujillo M. , Ludwig W. , Suzuki K-i. , Parte A. , Whitman W. . New York: Springer;.
-
Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. , other authors . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463––464 [CrossRef].
-
Williams S. T. , Cross T. . ( 1971;). Chapter XI Actinomycetes . Methods Microbiol 4: 295––334 [CrossRef].
-
Yamamura H. , Ashizawa H. , Nakagawa Y. , Hamada M. , Ishida Y. , Otoguro M. , Tamura T. , Hayakawa M. . ( 2011a;). Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample. J Antibiot (Tokyo) 64: 289––292 [CrossRef] [PubMed].
-
Yamamura H. , Ashizawa H. , Nakagawa Y. , Hamada M. , Ishida Y. , Otoguro M. , Tamura T. , Hayakawa M. . ( 2011b;). Actinomycetospora rishiriensis sp. nov., isolated from a lichen. Int J Syst Evol Microbiol 61: 2621––2625 [CrossRef] [PubMed].

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