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f Multilocus sequence analysis supports the taxonomic position of Astragalus glycyphyllos symbionts based on DNA–DNA hybridization

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  • Authors: Sebastian Gnat1​ ,  Wanda Małek2​ ,  Ewa Oleńska3​ ,  Sylwia Wdowiak-Wróbel2​ ,  Michał Kalita2​ ,  Jerzy Rogalski4​ , Magdalena Wójcik2​
    • VIEW AFFILIATIONS
    • 1​ 1​ Department of Veterinary Microbiology, University of Life Sciences, 13 Akademicka st., 20-950 Lublin, Poland 2​ 2​ Department of Genetics and Microbiology, University of Maria Curie-Sklodowska, 19 Akademicka st., 20-033 Lublin, Poland 3​ 3​ Department of Genetics and Evolution, University of Bialystok, 1J Ciolkowskiego st., 15-245 Bialystok, Poland 4​ 4​ Department of Biochemistry, University of Maria Curie-Sklodowska, 19 Akademicka st., 20-033 Lublin, Poland
  • Correspondence Wanda Małek [email protected]
  • First Published Online: 01 April 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1906-1912, doi: 10.1099/ijsem.0.000862
  • Subject: EVOLUTION, PHYLOGENY AND BIODIVERSITY
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Multilocus sequence analysis supports the taxonomic position of Astragalus glycyphyllos symbionts based on DNA–DNA hybridization, Page 1 of 1

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  • In this study, the phylogenetic relationship and taxonomic status of six strains, representing different phenons and genomic groups of Astragalus glycyphyllos symbionts, originating from Poland, were established by comparative analysis of five concatenated housekeeping gene sequences (atpD, dnaK, glnA, recA and rpoB), DNA–DNA hybridization and total DNA G+C content. Maximum-likelihood phylogenetic analysis of combined atpD, dnaK, glnA, recA and rpoB sequence data placed the studied bacteria into the clade comprising the genus Mesorhizobium. In the core gene phylograms, four A. glycyphyllos nodule isolates (AG1, AG7, AG15 and AG27) formed a cluster common with Mesorhizobium ciceri, whereas the two other A. glycyphyllos symbionts (AG17 and AG22) were grouped together with Mesorhizobium amorphae and M. septentrionale. The species position of the studied bacteria was clarified by DNA–DNA hybridization. The DNA–DNA relatedness between isolates AG1, AG7, AG15 and AG27 and reference strain M. ciceri USDA 3383 was 76.4–84.2 %, and all these A. glycyphyllos nodulators were defined as members of the genomospecies M. ciceri. DNA–DNA relatedness for isolates AG17 and AG22 and the reference strain M. amorphae ICMP 15022 was 77.5 and 80.1 %, respectively. We propose that the nodule isolates AG17 and AG22 belong to the genomic species M. amorphae. Additionally, it was found that the total DNA G+C content of the six test A. glycyphyllos symbionts was 59.4–62.1 mol%, within the range for species of the genus Mesorhizobium.

  • Abbreviations:
    ML maximum-likelihood
    MLSA multilocus sequence analysis
    SSU small subunit

© 2015 IUMS | Published by the Microbiology Society

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