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f sp. nov., isolated from roots of

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  • Authors: Yan-Qiong Li1​,† ,  Li Li2​,† ,  Yu-Song Fu3​ ,  Zhao-Qiong Cui1​ ,  Yan-Qing Duan4​ ,  Nimaichand Salam5​ ,  Jian-Wei Guo2​ ,  Wei Chen4​,† , Wen-Jun Li2​,3​,5​
    • VIEW AFFILIATIONS
    • 1​ 1​Kunming Medical University Haiyuan College,Kunming, 650106,PR China 2​ 2​Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences,Urumqi, 830011,PR China 3​ 3​Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091,PR China 4​ 4​China Tobacco Yunnan Industrial Co. Ltd,Kunming, 650231,PR China 5​ 5​State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275,PR China
  • Wei Chen [email protected]
  • First Published Online: 01 March 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1287-1292, doi: 10.1099/ijsem.0.000876
  • Subject: NEW TAXA - Actinobacteria
  • Cover date:
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  • A Gram-stain-positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007, was isolated from healthy roots of F. collected from Yili County, Xinjiang Province, north-west China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007 had highest similarities with members of the genus , including CAU 59 (96.98 % 16S rRNA gene sequence similarity), DSM 20419 (96.43 %) and THG-MD12 (96.14 %). The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60007 clustered with members of the genus , and formed a distinct clade with CAU 59. The polar lipids detected for strain EGI 60007 were phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.3 mol%. The chemotaxonomic features of strain EGI 60007 showed typical characteristics of the genus , with MK-9 as the respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan, and anteiso-C, anteiso-C and iso-C as the major fatty acids. The sugars of whole-cell hydrolysates were mainly mannose, rhamnose, ribose and glucose, and a minor amount of xylose. Based on the results of the phylogentic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60007 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 60007 ( = CGMCC 1.15081 = KCTC 39112 = DSM 29943).

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain EGI 60007 is KP721484.

  • A supplementary table and three supplementary figure are available with the online Supplementary Material.

  • Abbreviations:
    DPG diphosphatidylglycerol
    PG phosphatidylglycerol

© 2016 IUMS | Published by the Microbiology Society

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