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Volume 66, Issue 3

sp. nov., isolated from frozen soil Free

Abstract

Actinomycete strain ZLN234 was isolated from a frozen soil sample, which was collected from a glacier front in the Arctic. Chemotaxonomic and morphological characteristics were found to be typical of members of the genus . The strain formed a distinct phyletic line in the 16S rRNA gene tree and was closely related to ATCC 3336 (98.63 % similarity) and B7319 (98.56 %). Phylogenetic analysis based on the B gene also showed clearly that strain ZLN234 was different from all recognized species. The whole-cell sugars were galactose, glucose, ribose and rhamnose. The whole-cell-wall amino acids included alanine, glutamine, glycine and -diaminopimelic acid with a minor amount of -diaminopimelic acid. The characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and unknown phospholipids. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major cellular fatty acids comprised anteiso-C, iso-C and C with a small amount of iso-Cω7 and anteiso-C. The DNA G+C content of strain ZLN234 was 77.5 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, strain ZLN234 ( = CCTCC AA 2015005 = DSM 100713) is the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2016-03-01
2024-04-26
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References

  1. Annous B. A., Becker L. A., Bayles D. O., Labeda D. P., Wilkinson B. J. 1997; Critical role of anteiso-C15:0 fatty acid in the growth of Listeria monocytogenes at low temperatures. Appl Environ Microbiol 63:3887–3894[PubMed]
     [Google Scholar]
  2. Bonev B., Hooper J., Parisot J. 2008; Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother 61:1295–1301 [View Article][PubMed]
     [Google Scholar]
  3. Bredholdt H., Galatenko O. A., Engelhardt K., Fjaervik E., Terekhova L. P., Zotchev S. B. 2007; Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord. Norway: isolation, diversity and biological activity. Environ Microbiol 9:2756–2764 [View Article][PubMed]
     [Google Scholar]
  4. Castiglione F., Lazzarini A., Carrano L., Corti E., Ciciliato I., Gastaldo L., Candiani P., Losi D., Marinelli F., other authors. 2008; Determining the structure and mode of action of microbisporicin, a potent lantibiotic active against multiresistant pathogens. Chem Biol 15:22–31 [View Article][PubMed]
     [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  6. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
     [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  9. Garcia L. C., Martínez-Molina E., Trujillo M. E. 2010; Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum . Int J Syst Evol Microbiol 60:331–337 [View Article][PubMed]
     [Google Scholar]
  10. Goodfellow M., Fiedler H. P. 2010; A guide to successful bioprospecting: informed by actinobacterial systematics. Antonie van Leeuwenhoek 98:119–142 [View Article][PubMed]
     [Google Scholar]
  11. Groth I., Rodríguez C., Schütze B., Schmitz P., Leistner E., Goodfellow M. 2004; Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov. Int J Syst Evol Microbiol 54:2121–2129 [CrossRef]
     [Google Scholar]
  12. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145[PubMed]
     [Google Scholar]
  13. Kämpfer P., Labeda D. P. 2006; International Committee on Systematics of Prokaryotes; Subcommittee on the taxonomy of the Streptomycetaceae: minutes of the meeting, 25 July 2005, San Francisco, CA, USA. Int J Syst Evol Microbiol 56:495 [View Article]
     [Google Scholar]
  14. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [View Article][PubMed]
     [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  16. Korn-Wendisch F., Kutzner H. J. 1992; The family Streptomycetaceae . In The Prokaryotes pp 921–995Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
     [Google Scholar]
  17. Lam K. S. 2006; Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol 9:245–251 [View Article][PubMed]
     [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Evol Microbiol 20:435–443
     [Google Scholar]
  19. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy pp 227–291Edited by Dietz A., Thayer D. W. Arlington, VA: Society for Industrial Microbiology;
     [Google Scholar]
  20. Li W. J., Zhang Y. G., Zhang Y. Q., Tang S. K., Xu P., Xu L. H., Jiang C. L. 2005; Streptomyces sodiiphilus sp. nov., a novel alkaliphilic actinomycete. Int J Syst Evol Microbiol 55:1329–1333 [View Article][PubMed]
     [Google Scholar]
  21. Li L., Tang Y. L., Wei B., Xie Q. Y., Deng Z., Hong K. 2013; Micromonospora sonneratiae sp. nov., isolated from a root of Sonneratia apetala . Int J Syst Evol Microbiol 63:2383–2388 [View Article][PubMed]
     [Google Scholar]
  22. Lodders N., Kämpfer P. 2007; Streptomycetaceae: phylogeny, ecology and pathogenicity. www.els.net In Encyclopedia of Life Science pp 1–9 Chichester: Wiley;
     [Google Scholar]
  23. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H. P. 2013; When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195:413–418 [View Article][PubMed]
     [Google Scholar]
  24. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  25. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [View Article][PubMed]
     [Google Scholar]
  26. Pospiech A., Neumann B. 1995; A versatile quick-prep of genomic DNA from gram-positive bacteria. Trends Genet 11:217–218 [View Article][PubMed]
     [Google Scholar]
  27. Qu Z., Hong K. 2009; The identification of the G+C content of DNA by high performance liquid chromatography for mangrove actinomycetes. Biotechnol Bull S7:209–214
     [Google Scholar]
  28. Ramasamy D., Mishra A. K., Lagier J. C., Padhmanabhan R., Rossi M., Sentausa E., Raoult D., Fournier P. E. 2014; A polyphasic strategy incorporating genomic data for the taxonomic description of novel bacterial species. Int J Syst Evol Microbiol 64:384–391 [View Article][PubMed]
     [Google Scholar]
  29. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  30. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  31. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
     [Google Scholar]
  32. Shirling E. B., Gottlieb D. 1968a; Cooperative description of type cultures of Streptomyces II. Species descriptions from first study. Int J Syst Bacteriol 18:69–189 [View Article]
     [Google Scholar]
  33. Shirling E. B., Gottlieb D. 1968b; Cooperative description of type cultures of Streptomyces III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18:279–392 [View Article]
     [Google Scholar]
  34. Shirling E. B., Gottlieb D. 1969; Cooperative description of type cultures of Streptomyces IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19:391–512 [View Article]
     [Google Scholar]
  35. Shirling E. B., Gottlieb D. 1972; Cooperative description of type strains of Streptomyces V. Additional descriptions. Int J Syst Bacteriol 22:265–394 [View Article]
     [Google Scholar]
  36. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
     [Google Scholar]
  37. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  38. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  39. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
     [Google Scholar]
  40. Waksman S. A. 1967 The Actinomycetes. A Summary of Current Knowledge New York: Ronald Press;
     [Google Scholar]
  41. Waksman S. A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341[PubMed]
     [Google Scholar]
  42. Wang C., Xu X. X., Qu Z., Wang H. L., Lin H. P., Xie Q. Y., Ruan J. S., Hong K. 2011; Micromonospora rhizosphaerae sp. nov., isolated from mangrove rhizosphere soil. Int J Syst Evol Microbiol 61:320–324 [View Article][PubMed]
     [Google Scholar]
  43. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  44. Wiese J., Jiang Y., Tang S. K., Thiel V., Schmaljohann R., Xu L. H., Jiang C. L., Imhoff J. F. 2008; A new member of the family Micromonosporaceae, Planosporangium flavigriseum gen. nov., sp. nov.. Int J Syst Evol Microbiol 58:1324–1331 [View Article][PubMed]
     [Google Scholar]
  45. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813[PubMed]
     [Google Scholar]
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Streptomyces arcticus sp. nov., isolated from frozen soil
Int J Syst Evol Microbiol 66, 1482 (2016); https://doi.org/10.1099/ijsem.0.000907
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