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f sp. nov., isolated from frozen soil

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  • Authors: Li Zhang1​ ,  Changying Ruan1​ ,† ,  Fang Peng2​ ,  Zixin Deng1​ , Kui Hong1​
    • VIEW AFFILIATIONS
    • 1​ 1​ Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China 2​ 2​ China Center for Type Culture Collection, Wuhan, 430071, PR China
  • Kui Hong [email protected]
  • First Published Online: 01 March 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1482-1487, doi: 10.1099/ijsem.0.000907
  • Subject: NEW TAXA - Actinobacteria
  • Cover date:
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  • Actinomycete strain ZLN234 was isolated from a frozen soil sample, which was collected from a glacier front in the Arctic. Chemotaxonomic and morphological characteristics were found to be typical of members of the genus . The strain formed a distinct phyletic line in the 16S rRNA gene tree and was closely related to ATCC 3336 (98.63 % similarity) and B7319 (98.56 %). Phylogenetic analysis based on the B gene also showed clearly that strain ZLN234 was different from all recognized species. The whole-cell sugars were galactose, glucose, ribose and rhamnose. The whole-cell-wall amino acids included alanine, glutamine, glycine and -diaminopimelic acid with a minor amount of -diaminopimelic acid. The characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and unknown phospholipids. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major cellular fatty acids comprised anteiso-C, iso-C and C with a small amount of iso-Cω7 and anteiso-C. The DNA G+C content of strain ZLN234 was 77.5 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, strain ZLN234 ( = CCTCC AA 2015005 = DSM 100713) is the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

  • †

    Shanxi Zhashui Senior High School, Shanxi, 723000, PR China.

  • The GenBank/EMBL/DDJB accession numbers for the 16S rRNA and gyrB gene sequences of Streptomyces arcticus sp. nov. ZLN234T are KR013752 and KU285607, respectively.

  • Two supplementary tables and five supplementary figures are available with the online Supplementary Material.

  • Abbreviation:
    DDH DNA–DNA hybridization

© 2016 IUMS | Published by the Microbiology Society

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