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f sp. nov., isolated from frozen soil

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  • Authors: Li Zhang1​ ,  Changying Ruan1​ ,† ,  Fang Peng2​ ,  Zixin Deng1​ , Kui Hong1​
    • VIEW AFFILIATIONS
    • 1​ 1​ Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China 2​ 2​ China Center for Type Culture Collection, Wuhan, 430071, PR China
  • Kui Hong [email protected]
  • First Published Online: 01 March 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1482-1487, doi: 10.1099/ijsem.0.000907
  • Subject: NEW TAXA - Actinobacteria
  • Cover date:
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  • Actinomycete strain ZLN234 was isolated from a frozen soil sample, which was collected from a glacier front in the Arctic. Chemotaxonomic and morphological characteristics were found to be typical of members of the genus . The strain formed a distinct phyletic line in the 16S rRNA gene tree and was closely related to ATCC 3336 (98.63 % similarity) and B7319 (98.56 %). Phylogenetic analysis based on the B gene also showed clearly that strain ZLN234 was different from all recognized species. The whole-cell sugars were galactose, glucose, ribose and rhamnose. The whole-cell-wall amino acids included alanine, glutamine, glycine and -diaminopimelic acid with a minor amount of -diaminopimelic acid. The characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and unknown phospholipids. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major cellular fatty acids comprised anteiso-C, iso-C and C with a small amount of iso-Cω7 and anteiso-C. The DNA G+C content of strain ZLN234 was 77.5 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, strain ZLN234 ( = CCTCC AA 2015005 = DSM 100713) is the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

  • †

    Shanxi Zhashui Senior High School, Shanxi, 723000, PR China.

  • The GenBank/EMBL/DDJB accession numbers for the 16S rRNA and gyrB gene sequences of Streptomyces arcticus sp. nov. ZLN234T are KR013752 and KU285607, respectively.

  • Two supplementary tables and five supplementary figures are available with the online Supplementary Material.

  • Abbreviation:
    DDH DNA–DNA hybridization

© 2016 IUMS | Published by the Microbiology Society

  • Annous B. A. , Becker L. A. , Bayles D. O. , Labeda D. P. , Wilkinson B. J. . ( 1997;). Critical role of anteiso-C15:0 fatty acid in the growth of Listeria monocytogenes at low temperatures. Appl Environ Microbiol 63: 3887––3894 [PubMed].
     [Google Scholar]
  • Bonev B. , Hooper J. , Parisot J. . ( 2008;). Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother 61: 1295––1301 [CrossRef] [PubMed].
     [Google Scholar]
  • Bredholdt H. , Galatenko O. A. , Engelhardt K. , Fjaervik E. , Terekhova L. P. , Zotchev S. B. . ( 2007;). Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord. Norway: isolation, diversity and biological activity. Environ Microbiol 9: 2756––2764 [CrossRef] [PubMed].
     [Google Scholar]
  • Castiglione F. , Lazzarini A. , Carrano L. , Corti E. , Ciciliato I. , Gastaldo L. , Candiani P. , Losi D. , Marinelli F. , other authors . ( 2008;). Determining the structure and mode of action of microbisporicin, a potent lantibiotic active against multiresistant pathogens. Chem Biol 15: 22––31 [CrossRef] [PubMed].
     [Google Scholar]
  • Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57: 2259––2261 [CrossRef] [PubMed].
     [Google Scholar]
  • Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221––230 [CrossRef] [PubMed].
     [Google Scholar]
  • Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368––376 [CrossRef] [PubMed].
     [Google Scholar]
  • Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406––416 [CrossRef].
     [Google Scholar]
  • Garcia L. C. , Martínez-Molina E. , Trujillo M. E. . ( 2010;). Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum . Int J Syst Evol Microbiol 60: 331––337 [CrossRef] [PubMed].
     [Google Scholar]
  • Goodfellow M. , Fiedler H. P. . ( 2010;). A guide to successful bioprospecting: informed by actinobacterial systematics. Antonie van Leeuwenhoek 98: 119––142 [CrossRef] [PubMed].
     [Google Scholar]
  • Groth I. , Rodríguez C. , Schütze B. , Schmitz P. , Leistner E. , Goodfellow M. . ( 2004;). Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov. Int J Syst Evol Microbiol 54: 2121––2129.[CrossRef]
    [Google Scholar]
  • Jones K. L. . ( 1949;). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57: 141––145 [PubMed].
     [Google Scholar]
  • Kämpfer P. , Labeda D. P. . ( 2006;). International Committee on Systematics of Prokaryotes; Subcommittee on the taxonomy of the Streptomycetaceae: minutes of the meeting, 25 July 2005, San Francisco, CA, USA. Int J Syst Evol Microbiol 56: 495 [CrossRef].
     [Google Scholar]
  • Kämpfer P. , Steiof M. , Dott W. . ( 1991;). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21: 227––251 [CrossRef] [PubMed].
     [Google Scholar]
  • Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716––721 [CrossRef] [PubMed].
     [Google Scholar]
  • Korn-Wendisch F. , Kutzner H. J. . ( 1992;). The family Streptomycetaceae . . In The Prokaryotes, pp. 921––995. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . New York: Springer;.
     [Google Scholar]
  • Lam K. S. . ( 2006;). Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol 9: 245––251 [CrossRef] [PubMed].
     [Google Scholar]
  • Lechevalier M. P. , Lechevalier H. . ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Evol Microbiol 20: 435––443.
     [Google Scholar]
  • Lechevalier M. P. , Lechevalier H. A. . ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, pp. 227––291. Edited by Dietz A. , Thayer D. W. . Arlington, VA: Society for Industrial Microbiology;.
     [Google Scholar]
  • Li W. J. , Zhang Y. G. , Zhang Y. Q. , Tang S. K. , Xu P. , Xu L. H. , Jiang C. L. . ( 2005;). Streptomyces sodiiphilus sp. nov., a novel alkaliphilic actinomycete. Int J Syst Evol Microbiol 55: 1329––1333 [CrossRef] [PubMed].
     [Google Scholar]
  • Li L. , Tang Y. L. , Wei B. , Xie Q. Y. , Deng Z. , Hong K. . ( 2013;). Micromonospora sonneratiae sp. nov., isolated from a root of Sonneratia apetala . Int J Syst Evol Microbiol 63: 2383––2388 [CrossRef] [PubMed].
     [Google Scholar]
  • Lodders N. , Kämpfer P. . ( 2007;). Streptomycetaceae: phylogeny, ecology and pathogenicity. .www.els.net In Encyclopedia of Life Science, pp. 1––9 Chichester: Wiley;.
     [Google Scholar]
  • Meier-Kolthoff J. P. , Göker M. , Spröer C. , Klenk H. P. . ( 2013;). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195: 413––418 [CrossRef] [PubMed].
     [Google Scholar]
  • Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233––241 [CrossRef].
     [Google Scholar]
  • Nakajima Y. , Kitpreechavanich V. , Suzuki K. , Kudo T. . ( 1999;). Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49: 1761––1767 [CrossRef] [PubMed].
     [Google Scholar]
  • Pospiech A. , Neumann B. . ( 1995;). A versatile quick-prep of genomic DNA from gram-positive bacteria. Trends Genet 11: 217––218 [CrossRef] [PubMed].
     [Google Scholar]
  • Qu Z. , Hong K. . ( 2009;). The identification of the G+C content of DNA by high performance liquid chromatography for mangrove actinomycetes. Biotechnol Bull S7: 209––214.
     [Google Scholar]
  • Ramasamy D. , Mishra A. K. , Lagier J. C. , Padhmanabhan R. , Rossi M. , Sentausa E. , Raoult D. , Fournier P. E. . ( 2014;). A polyphasic strategy incorporating genomic data for the taxonomic description of novel bacterial species. Int J Syst Evol Microbiol 64: 384––391 [CrossRef] [PubMed].
     [Google Scholar]
  • Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406––425 [PubMed].
     [Google Scholar]
  • Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  • Shirling E. B. , Gottlieb D. . ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313––340 [CrossRef].
     [Google Scholar]
  • Shirling E. B. , Gottlieb D. . ( 1968a;). Cooperative description of type cultures of Streptomyces II. Species descriptions from first study. Int J Syst Bacteriol 18: 69––189 [CrossRef].
     [Google Scholar]
  • Shirling E. B. , Gottlieb D. . ( 1968b;). Cooperative description of type cultures of Streptomyces III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18: 279––392 [CrossRef].
     [Google Scholar]
  • Shirling E. B. , Gottlieb D. . ( 1969;). Cooperative description of type cultures of Streptomyces IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19: 391––512 [CrossRef].
     [Google Scholar]
  • Shirling E. B. , Gottlieb D. . ( 1972;). Cooperative description of type strains of Streptomyces V. Additional descriptions. Int J Syst Bacteriol 22: 265––394 [CrossRef].
     [Google Scholar]
  • Stackebrandt E. , Ebers J. . ( 2006;). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33: 152––155.
     [Google Scholar]
  • Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731––2739 [CrossRef] [PubMed].
     [Google Scholar]
  • Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876––4882 [CrossRef] [PubMed].
     [Google Scholar]
  • Tindall B. J. , Rosselló-Móra R. , Busse H. J. , Ludwig W. , Kämpfer P. . ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60: 249––266 [CrossRef] [PubMed].
     [Google Scholar]
  • Waksman S. A. . ( 1967;). The Actinomycetes. A Summary of Current Knowledge New York: Ronald Press;.
     [Google Scholar]
  • Waksman S. A. , Henrici A. T. . ( 1943;). The nomenclature and classification of the actinomycetes. J Bacteriol 46: 337––341 [PubMed].
     [Google Scholar]
  • Wang C. , Xu X. X. , Qu Z. , Wang H. L. , Lin H. P. , Xie Q. Y. , Ruan J. S. , Hong K. . ( 2011;). Micromonospora rhizosphaerae sp. nov., isolated from mangrove rhizosphere soil. Int J Syst Evol Microbiol 61: 320––324 [CrossRef] [PubMed].
     [Google Scholar]
  • Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. , other authors . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463––464 [CrossRef].
     [Google Scholar]
  • Wiese J. , Jiang Y. , Tang S. K. , Thiel V. , Schmaljohann R. , Xu L. H. , Jiang C. L. , Imhoff J. F. . ( 2008;). A new member of the family Micromonosporaceae, Planosporangium flavigriseum gen. nov., sp. nov.. Int J Syst Evol Microbiol 58: 1324––1331 [CrossRef] [PubMed].
     [Google Scholar]
  • Williams S. T. , Goodfellow M. , Alderson G. , Wellington E. M. , Sneath P. H. , Sackin M. J. . ( 1983;). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129: 1743––1813 [PubMed].
     [Google Scholar]
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