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Volume 66, Issue 6

sp. nov., isolated from deep seawater Free

Abstract

A novel Gram-stain-negative, rod-shaped (1.0–1.2×2.0–8.0 µm), non-motile without flagella strain, designated HSF7, was isolated from deep seawater. Strain HSF7 was able to grow at 20–40 °C (optimum 35 °C), pH 5.5–9.0 (optimum pH 6.5) and 0–10 % (w/v) NaCl (optimum 2 %). The G+C content of the genomic DNA was 69 mol%. Bacteriochlorophyll and poly--hydroxybutyrate (PHB) granules were not found. The major fatty acids were Cω7 (69.3 %), C (9.1 %) and C cyclo ω8 (6.6 %). The polar lipids were phosphatidylglycerol, three unknown aminophospholipids, an unknown phospholipid, an unknown aminolipid and two unknown lipids. The only isoprenoid quinone was Q-10. 16S rRNA gene sequence analysis revealed that strain HSF7 was most closely related to DSM 19539, DSM 18839, DSM 18348 and MCCC 1A02083with pairwise sequence similarities of 95.56 %, 95.21 %, 93.64 % and 92.65 %, respectively. On the basis of genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain HSF7 represents a novel species of the genus , or which the name sp. nov. is proposed. The type strain is HSF7(=KCTC 42651=MCCC 1K01158).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Proteobacteria novel species
© 2016 IUMS
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2016-06-10
2024-04-26
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References

  1. Claus D. 1992; A standardized Gram staining procedure. World J Microb Biot 8:451–452 [View Article]
     [Google Scholar]
  2. Euzéby J. P. 1997; List of bacterial names with standing in nomenclature: A folder available on the internet. Int J Syst Bacteriol 47:590–592 [View Article][PubMed]
     [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  4. Fitch W. M. 1987; Citation Classic - Toward defining the course of evolution: minimum change for a specific tree topology. CC/Agri Biol Environ Sci14
     [Google Scholar]
  5. Kovacs N. 1956; Identification of pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article]
     [Google Scholar]
  6. Kumar P. A., Srinivas T. N., Manasa P., Madhu S., Shivaji S. 2012; Lutibaculum baratangense gen. nov., sp. nov., a proteobacterium isolated from a mud volcano. Int J Syst Evol Microbiol 62:2025–2031 [View Article][PubMed]
     [Google Scholar]
  7. Kuykendall L. D., Roy M. A., O'neill J. J., Devine T. E. 1998; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [View Article]
     [Google Scholar]
  8. Lai Q., Yuan J., Wu C., Shao Z. 2009a; Oceanibaculum indicum gen. nov., sp. nov., isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 59:1733–1737 [View Article]
     [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  10. Mesquita D. P., Amaral A. L., Leal C., Oehmen A., Reis M. A., Ferreira E. C. 2015; Polyhydroxyalkanoate granules quantification in mixed microbial cultures using image analysis: Sudan black B versus nile blue A staining. Anal Chim Acta 865:8–15 [View Article][PubMed]
     [Google Scholar]
  11. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  12. Pan J., Sun C., Zhang X. Q., Huo Y. Y., Zhu X. F., Wu M. 2014; Paracoccus sediminis sp. nov., isolated from pacific ocean marine sediment. Int J Syst Evol Microbiol 64:2512–2516 [View Article][PubMed]
     [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  14. Sorokina A. Y., Chernousova E. Y., Dubinina G. A. 2012; Ferrovibrio denitrificans gen. nov., sp. nov., a novel neutrophilic facultative anaerobic Fe(ii)-oxidizing bacterium. FEMS Microbiol Lett 335:19–25 [View Article][PubMed]
     [Google Scholar]
  15. Sun C., Pan J., Zhang X. Q., Su Y., Wu M. 2015; Pseudoroseovarius zhejiangensis gen. nov., sp. nov., a novel alpha-proteobacterium isolated from the chemical wastewater, and reclassification of Roseovarius crassostreae as Pseudoroseovarius crassostreae comb. nov., Roseovarius sediminilitoris as Pseudoroseovarius sediminilitoris comb. nov. and Roseovarius halocynthiae as Pseudoroseovarius halocynthiae comb. nov. Antonie van Leeuwenhoek 108:291–299 [View Article][PubMed]
     [Google Scholar]
  16. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [View Article][PubMed]
     [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  18. Urios L., Michotey V., Intertaglia L., Lesongeur F., Lebaron P. 2008; Nisaea denitrificans gen. nov., sp. nov. and Nisaea nitritireducens sp. nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 58:2336–2341 [View Article][PubMed]
     [Google Scholar]
  19. Urios L., Michotey V., Intertaglia L., Lesongeur F., Lebaron P. 2010; Thalassobaculum salexigens sp. nov., a new member of the family Rhodospirillaceae from the NW Mediterranean Sea, and emended description of the genus Thalassobaculum . Int J Syst Evol Microbiol 60:209–213 [View Article][PubMed]
     [Google Scholar]
  20. Williams S. T., Davies F. L. 1965; Use of antibiotics for selective isolation and enumeration of actinomycetes in soil. J Gen Microbiol 38:251–261 [CrossRef]
     [Google Scholar]
  21. Wu X. Y., Zheng G., Zhang W. W., Xu X. W., Wu M., Zhu X. F. 2010; Amphibacillus jilinensis sp. nov., a facultatively anaerobic, alkaliphilic bacillus from a soda lake. Int J Syst Evol Microbiol 60:2540–2543 [View Article][PubMed]
     [Google Scholar]
  22. Xu X. W., Huo Y. Y., Wang C. S., Oren A., Cui H. L., Vedler E., Wu M. 2011; Pelagibacterium halotolerans gen. nov., sp. nov. and Pelagibacterium luteolum sp. nov., novel members of the family Hyphomicrobiaceae . Int J Syst Evol Microbiol 61:1817–1822 [View Article][PubMed]
     [Google Scholar]
  23. Zhang G. I., Hwang C. Y., Cho B. C. 2008; Thalassobaculum litoreum gen nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. Int J Syst Evol Microbiol 58:479–485 [View Article][PubMed]
     [Google Scholar]
  24. Zhang D. C., Liu H. C., Zhou Y. G., Schinner F., Margesin R. 2011; Tistrella bauzanensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 61:2227–2230 [View Article][PubMed]
     [Google Scholar]
  25. Zhang W. Y., Huo Y. Y., Zhang X. Q., Zhu X. F., Wu M. 2013; Halolamina salifodinae sp. nov. and Halolamina salina sp. nov., two extremely halophilic archaea isolated from a salt mine. Int J Syst Evol Microbiol 63:4380–4385 [CrossRef]
     [Google Scholar]
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Thalassobaculum fulvum sp. nov., isolated from deep seawater
Int J Syst Evol Microbiol 66, 2186 (2016); https://doi.org/10.1099/ijsem.0.001008
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