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f Mucilaginibacter pocheonensis sp. nov., with ginsenoside-converting activity, isolated from soil of a ginseng-cultivating field

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  • Authors: Yan Zhao1​ ,2​ ,† ,  Hyung-Gwan Lee3​ ,† ,  Soo-Ki Kim4​ ,  Hongshan Yu2​ ,  Fengxie Jin2​ , Wan-Taek Im1​ ,5​
    • VIEW AFFILIATIONS
    • 1​ 1​ Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do 456-749, South Korea 2​ 2​ College of Biotechnology, Dalian Polytechnic University, Qinggong-yuan No. 1, Ganjingzi-qu, Dalian 116034, PR China 3​ 3​ Bioenergy and Biochemical Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea 4​ 4​ Department of Animal Science and Technology, Konkuk University, Seoul 05029, Republic of Korea 5​ 5​ Genomic Informatics Center, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea
  • Correspondence Wan-Taek Im [email protected]
  • First Published Online: 01 August 2016, International Journal of Systematic and Evolutionary Microbiology 66: 2862-2868, doi: 10.1099/ijsem.0.001069
  • Subject: New taxa - Bacteroidetes
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Mucilaginibacter pocheonensis sp. nov., with ginsenoside-converting activity, isolated from soil of a ginseng-cultivating field, Page 1 of 1

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  • A Gram-reaction-negative, aerobic, heterotrophic, non-motile, non-spore-forming, rod-shaped bacterial strain, designated Gsoil 032, was isolated from soil of a ginseng field in Pocheon Province, South Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain Gsoil 032 grew at 10–42 °C and at pH 5.0–10.0 on R2A agar medium. Strain Gsoil 032 possessed β-glucosidase activity, which was responsible for its ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) to compound K. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 032 was shown to belong to the family Sphingobacteriaceae and to be related to Mucilaginibacter sabulilitoris SMS-12 (97.6 % sequence similarity) and Mucilaginibacter lappiensis ANJLI2 (97.1 %) The G+C content of the genomic DNA was 44.4 mol%. The predominant respiratory quinone was menaquinone MK-7 and the major fatty acids were summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), iso-C15 : 0 and iso-C17 : 0 3-OH. The major polar lipid detected was phosphatidylethanolamine, while the minor polar lipids were various unidentified aminophospholipids, unidentified phospholipids and unidentified polar lipids. DNA and chemotaxonomic data supported the affiliation of strain Gsoil 032 to the genus Mucilaginibacter . Strain Gsoil 032could be differentiated genotypically and phenotypically from recognized species of the genus Mucilaginibacter . The isolate therefore represents a novel species, for which the name Mucilaginibacter pocheonensis sp. nov. is proposed, with the type strain Gsoil 032 (=KCTC 12641=LMG 23495).

  • †

    These authors contributed equally to this work.

  • The NCBI GenBank accession number for the 16S rRNA gene sequence of strain Gsoil 032 is AB267718.

  • Five supplementary figures and one supplementary table are available with the online Supplementary Material.

  • Abbreviation:
    C-K compound K

© 2016 IUMS | Published by the Microbiology Society

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