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f Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

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Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces, Page 1 of 1

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  • Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1 and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1 ω9, C18 : 1 ω9 aldehyde, C16 : 0 and C16 : 1 ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4–56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans Fae, 91.3/91.2 % with Gemmiger formicilis ATCC 27749 and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae , for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1 (=DSM 100348=VKM B-2901).

  • †

    APC Microbiome Institute, University College Cork, Cork, Ireland

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 585-1 and 668 are KM098109 and KM098110, respectively.

  • Seven supplementary tables are available with the online Supplementary Material.

  • Abbreviations:
    ANI average nucleotide identity
    CFA cellular fatty acid
    IBD inflammatory bowel disease
    NJ neighbour-joining

© 2016 IUMS | Published by the Microbiology Society

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2016-08-01
2019-02-19
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