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f Dyadobacter endophyticus sp. nov., an endophytic bacterium isolated from maize root

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  • Authors: Jun-lian Gao1​,† ,  Pengbo Sun2​,† ,  Xu-ming Wang1​ ,  Tian-lei Qiu1​ ,  Fan-yang Lv3​ ,  Mei Yuan3​ ,  Ming-ming Yang1​ , Jian-guang Sun3​
    • VIEW AFFILIATIONS
    • 1​ 1​Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, PR China 2​ 2​State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, PR China 3​ 3​Key Laboratory of Microbial Resource, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China
  • Correspondence Jian-guang Sun [email protected]
  • First Published Online: 01 October 2016, International Journal of Systematic and Evolutionary Microbiology 66: 4022-4026, doi: 10.1099/ijsem.0.001304
  • Subject: New taxa - Bacteroidetes
  • Received:
  • Accepted:
  • Cover date:
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Dyadobacter endophyticus sp. nov., an endophytic bacterium isolated from maize root, Page 1 of 1

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  • A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain, designated 65, was isolated from surface-sterilized root tissue of maize, collected from Fangshan District of Beijing, People’s Republic of China, and was subjected to a taxonomic study by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 65 belonged to the genus Dyadobacter and had highest 16S rRNA gene sequence similarity to Dyadobacter jiangsuensis CGMCC 1.12969 (99.1 %), Dyadobacter beijingensis CGMCC 1.6375 (98.8 %), Dyadobacter fermentans DSM 18053 (98.6 %) and Dyadobacter soli KCTC 22481 (98.6 %). However, the new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to D. jiangsuensis CGMCC 1.12969 (18.2±1.3 %), D. beijingensis CGMCC 1.6375 (14.2±2.0 %), D. fermentans DSM 18053 (14.1±2.0 %) and D. soli KCTC 22481 (13.8±0.6 %). The predominant respiratory quinone was menaquinone-7 (MK-7) and the major cellular fatty acids were summed feature 3 (C16  :  1 ω7c and/or iso-C15 : 0 2-OH), iso-C15  :  0, iso-C17  :  0 3-OH, C16  :  1 ω5c, iso-C15  :  0 3-OH, C16  :  0 3-OH and C16 : 0. The polar lipid profile of strain 65 revealed the presence of phosphatidylethanolamine, four aminolipids and two unidentified phospholipids. The DNA G+C content was 46.6 mol%. The results of physiological and biochemical tests and the differences in the fatty acid profiles allowed the clear phenotypic differentiation of strain 65 from closely related species of the genus Dyadobacter . Strain 65 thus represents a novel species within the genus Dyadobacter , for which the name Dyadobacter endophyticus sp. nov. is proposed. The type strain is 65 (=CGMCC 1.15288=DSM 100786).

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDJB accession number for the 16S rRNA gene sequence of strain 65 is KT003984.

  • Four supplementary figures are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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