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f Reclassification of Pseudomonas sp. PB-6250 as Lysobacter firmicutimachus sp. nov.

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  • Authors: Henrike Miess1​,2​ ,  Stefanie van Trappen3​ ,  Ilse Cleenwerck3​ ,  Paul De Vos3​ , Harald Gross1​,2​
    • VIEW AFFILIATIONS
    • 1​ 1​Department of Pharmaceutical Biology, Pharmaceutical Institute, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen, Germany 2​ 2​German Centre for Infection Research (DZIF), partner site Tübingen, 72076 Tübingen, Germany 3​ 3​BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Faculty of Sciences, Ledeganckstraat 35, 9000 Ghent, Belgium
  • Correspondence Harald Gross [email protected]
  • First Published Online: 01 October 2016, International Journal of Systematic and Evolutionary Microbiology 66: 4162-4166, doi: 10.1099/ijsem.0.001329
  • Subject: New taxa - Proteobacteria
  • Received:
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Reclassification of Pseudomonas sp. PB-6250 as Lysobacter firmicutimachus sp. nov., Page 1 of 1

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  • Strain PB-6250, isolated from soil in Japan, was first identified in 1992. In contrast to its original taxonomic classification, its 16S rRNA gene sequence showed the highest similarity (99.2 %) to the sequence of Lysobacter enzymogenes DSM 2043, with Lysobacter antibioticus DSM 2044 being the next most closely related species (98.7 %) with a validly published name. Chemotaxonomic data (fatty acid profile, quinone and polar lipid composition) and the G+C content of strain PB-6250 were compared with those of the closely related type strains L. enzymogenes LMG 8762, L. antibioticus LMG 8760, L . capsici DSM 19286 and L. gummosus LMG 8763; this supported the affiliation of strain PB-6250 to the genus Lysobacter . Phylogenetic analyses, DNA−DNA-hybridization data, biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain PB-6250 from species of Lysobacter with validly published names. Strain PB-6250, therefore represents a novel species, for which the name Lysobacter firmicutimachus sp. nov. is proposed. The type strain is PB-6250 (=LMG 28994=DSM 102073).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Lysobacter firmicutimachus is KU593484.

  • One supplementary figure is available with the online Supplementary Material.

  • Abbreviations:
    DPG diphosphatidylglycerol
    FAME Fatty acid methyl esters
    PE phosphatidylethanolamine
    PG Phosphatidylglycerol
    PMME Phosphatidylmonomethylethanolamine

© 2016 IUMS | Published by the Microbiology Society

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2019-02-20
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