Reclassification of Pseudomonas sp. PB-6250T as Lysobacter firmicutimachus sp. nov.

Henrike Miess; Stefanie van Trappen; Ilse Cleenwerck; Paul De Vos; Harald Gross

doi:10.1099/ijsem.0.001329

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f Reclassification of Pseudomonas sp. PB-6250T as Lysobacter firmicutimachus sp. nov.

Authors: Henrike Miess^1,2 , Stefanie van Trappen³ , Ilse Cleenwerck³ , Paul De Vos³ , Harald Gross^1,2
- VIEW AFFILIATIONS
- ¹ 1Department of Pharmaceutical Biology, Pharmaceutical Institute, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen, Germany ² 2German Centre for Infection Research (DZIF), partner site Tübingen, 72076 Tübingen, Germany ³ 3BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Faculty of Sciences, Ledeganckstraat 35, 9000 Ghent, Belgium
Correspondence Harald Gross [email protected]
First Published Online: 01 October 2016, International Journal of Systematic and Evolutionary Microbiology 66: 4162-4166, doi: 10.1099/ijsem.0.001329
Subject: New taxa - Proteobacteria

Received: 11/02/2016
Accepted: 19/07/2016
Cover date: 01/10/2016

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Reclassification of Pseudomonas sp. PB-6250T as Lysobacter firmicutimachus sp. nov., Page 1 of 1

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Strain PB-6250T, isolated from soil in Japan, was first identified in 1992. In contrast to its original taxonomic classification, its 16S rRNA gene sequence showed the highest similarity (99.2 %) to the sequence of Lysobacter enzymogenes DSM 2043T, with Lysobacter antibioticus DSM 2044T being the next most closely related species (98.7 %) with a validly published name. Chemotaxonomic data (fatty acid profile, quinone and polar lipid composition) and the G+C content of strain PB-6250T were compared with those of the closely related type strains L. enzymogenes LMG 8762T, L. antibioticus LMG 8760T, L . capsici DSM 19286T and L. gummosus LMG 8763T; this supported the affiliation of strain PB-6250T to the genus Lysobacter . Phylogenetic analyses, DNA−DNA-hybridization data, biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain PB-6250T from species of Lysobacter with validly published names. Strain PB-6250T, therefore represents a novel species, for which the name Lysobacter firmicutimachus sp. nov. is proposed. The type strain is PB-6250T (=LMG 28994T=DSM 102073T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Lysobacter firmicutimachus T is KU593484.
One supplementary figure is available with the online Supplementary Material.

Keyword(s): Plusbacin, Lysobacter

Abbreviations:

DPG	diphosphatidylglycerol
FAME	Fatty acid methyl esters
PE	phosphatidylethanolamine
PG	Phosphatidylglycerol
PMME	Phosphatidylmonomethylethanolamine

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