1887
  1. Home
  2. Publications
  3. International Journal of Systematic and Evolutionary Microbiology
  4. Volume 66, Issue 12
  5. Article

f Emticicia fontis sp. nov., isolated from a freshwater pond

HTML
50.74 Kb
PDF
155.84 Kb
  • Received:
  • Accepted:
  • Cover date:
Preview this:
Free Preview thumbnail Magnify
Zoom in
Zoomout

Emticicia fontis sp. nov., isolated from a freshwater pond, Page 1 of 1

| /docserver/preview/fulltext/ijsem/66/12/5161_ijsem001490-1.gif

  • A bacterial strain, designated IMCC1731, was isolated from an eutrophic freshwater pond and characterized using a polyphasic taxonomic approach. Cellular growth occurred at pH 6–9 (optimum, pH 8.0), at 10–30 °C (optimum, 25 °C) and with 0–1 % (w/v) NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain IMCC1731 belonged to the genus Emticicia in the family Cytophagaceae and was most closely related to Emticicia ginsengisoli Gsoil 085 (98.1 %) followed by Emticicia paludis HMF3850 (95.0 %), Emticicia oligotrophica DSM 17448 (94.7 %), Emticicia aquatica HMF2925 (94.4 %) and Emticicia sediminis JBR12 (94.0 %). The DNA–DNA relatedness between strain IMCC1731 and E. ginsengisoli Gsoil 085 was 47±4 %. The DNA G+C content of strain IMCC1731 was 37.7 mol%. The predominant cellular fatty acids were summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and iso-C15 : 0. Based on the physiological and chemotaxonomic characteristics, DNA–DNA relatedness and 16S rRNA gene phylogeny, stain IMCC1731 is considered to represent a novel species of the genus Emticicia , for which the name Emticicia fontis sp. nov. is proposed. The type strain is IMCC1731 (=KCTC 52248=JCM 31373).

  • †

    Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain IMCC1731 is DQ664246.

  • Two supplementary figures are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

  • Bernardet J. F., Nakagawa Y., Holmes B.. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52: 1049––1070. [CrossRef] [PubMed]
    [Google Scholar]
  • Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17: 368––376. [CrossRef] [PubMed]
    [Google Scholar]
  • Fitch W. M..( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20: 406––416. [CrossRef]
    [Google Scholar]
  • Gonzalez J. M., Saiz-Jimenez C..( 2002;). A fluorimetric method for the estimation of G+C mol% content in micro-organisms by thermal denaturation temperature. . Environ Microbiol 4: 770––773.[PubMed] [CrossRef]
    [Google Scholar]
  • Joung Y., Seo M., Kang H., Kim H., Cho J. C., Joh K..( 2015;). Emticicia aquatica sp. nov., a species of the family Cytophagaceae isolated from fresh water. . Int J Syst Evol Microbiol 65: 4358––4362. [CrossRef] [PubMed]
    [Google Scholar]
  • Jukes T. H., Cantor C. R..( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21––32. Edited by Munro H. N.. New York:: Academic Press;.[CrossRef]
    [Google Scholar]
  • Kang H., Kim H., Joung Y., Joh K..( 2016;). Emticicia paludis sp. nov., isolated from wetland freshwater. . Int J Syst Evol Microbiol 66: 3383––3387 [CrossRef]
    [Google Scholar]
  • Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716––721. [CrossRef] [PubMed]
    [Google Scholar]
  • Liu Q. M., Ten L. N., Yu H. S., Jin F. X., Im W. T., Lee S. T..( 2008;). Emticicia ginsengisoli sp. nov., a species of the family ‘Flexibacteraceae’ isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 58: 1100––1105. [CrossRef] [PubMed]
    [Google Scholar]
  • Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al.( 2004;). ARB: a software environment for sequence data. . Nucleic Acids Res 32: 1363––1371. [CrossRef] [PubMed]
    [Google Scholar]
  • Ludwig W., Euzéby J., Whitman W. B..( 2010;). Taxonomic outlines of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. . In Bergey’s Manual of Systematic Bacteriology,vol. 4 pp. 21––24. Edited by Whitman W.. Baltimore:: The Williams & Wilkins Co. Springer;.[CrossRef]
    [Google Scholar]
  • Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H..( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2: 233––241. [CrossRef]
    [Google Scholar]
  • Park S. E., Lee S., Kim H., Ahn T. Y..( 2015;). Emticicia sediminis sp. nov. isolated from sediment of a shallow stream. . Int J Syst Evol Microbiol 65: 2496––2499. [CrossRef] [PubMed]
    [Google Scholar]
  • Saha P., Chakrabarti T..( 2006;). Emticicia oligotrophica gen. nov., sp. nov., a new member of the family ‘Flexibacteraceae’, phylum Bacteroidetes. . Int J Syst Evol Microbiol 56: 991––995. [CrossRef] [PubMed]
    [Google Scholar]
  • Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406––425.[PubMed]
    [Google Scholar]
  • Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725––2729. [CrossRef] [PubMed]
    [Google Scholar]
  • Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R..( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330––393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R.. Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  • Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E..( 1987;). International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37: 463––464.[CrossRef]
    [Google Scholar]
http://sgm.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001490
Loading

Supplementary Data

 Data loading....

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001490
2016-12-01
2019-02-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5161.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001490&mimeType=html&fmt=ahah

Author and Article Information

 Figure data loading....

Footnotes:

Validated antibodies in this article

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error