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f Frigoribacterium salinisoli sp. nov., isolated from saline soil, transfer of Frigoribacterium mesophilum to Parafrigoribacterium gen. nov. as Parafrigoribacterium mesophilum comb. nov.

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Frigoribacterium salinisoli sp. nov., isolated from saline soil, transfer of Frigoribacterium mesophilum to Parafrigoribacterium gen. nov. as Parafrigoribacterium mesophilum comb. nov., Page 1 of 1

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  • A Gram-stain-positive, short-rod, aerobic bacterium, designated as strain LAM9155, was isolated from saline soil sample collected from Lingxian County, Shandong Province, PR China. The strain grew optimally at 25–30 °C, pH 7.0 and 0.5 % (w/v) NaCl. The 16S rRNA gene sequence analysis revealed that strain LAM9155 belonged to the genus Frigoribacterium and was closely related to Frigoribacterium endophyticum EGI 6500707 (99.4 %), Frigoribacterium faeni 801 (98.6 %) and Frigoribacterium mesophilum MSL-08 (96.2 %). The DNA–DNA hybridization values between strain LAM9155 and F. endophyticum JCM 30093 and between strain LAM9155 and F. faeni DSM 10309 were 40.2±2.1 and 32.8±1.6 %, respectively. The major fatty acids of LAM9155 were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The cell-wall analysis showed the B-type peptidoglycan containing alanine, glutamate, glycine, serine and lysine and that the cell wall contained the sugars galactose and ribose. The genomic DNA G+C content of strain LAM9155 was 68.2 mol%. The predominant menaquinone was MK-9. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown glycolipid and four unknown lipids. Based on the DNA–DNA hybridization and phenotypic, phylogenetic and chemotaxonomic properties, strain LAM9155 could be distinguished from the recognized species of the genus Frigoribacterium and was suggested to represent a novel species, for which the name Frigoribacterium salinisoli sp. nov. is proposed. The type strain is LAM9155 (=ACCC 19902=JCM 30848). Moreover, the transfer of F . mesophilum Dastager et al. 2008 to Parafrigoribacterium gen. nov. as Parafrigoribacterium mesophilum comb. nov. (type strain MSL-08=DSM 19442=KCTC 19311) is also proposed.

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LAM9155 is KX094417.

  • One supplementary table and three supplementary figures are available with the online Supplementary Material.

  • Abbreviations:
    DPG diphosphatidylglycerol
    PG phosphatidylglycerol

© 2016 IUMS | Published by the Microbiology Society

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2016-12-01
2019-02-22
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