Williamsia spongiae sp. nov., an actinomycete isolated from the marine sponge Amphimedon viridis

Cláudia Beatriz Afonso de Menezes; Rafael Sanches Afonso; Wallace Rafael de Souza; Márcia Parma; Itamar Soares de Melo; Tiago Domingues Zucchi; Fabiana Fantinatti-Garboggini

doi:10.1099/ijsem.0.001796

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f Williamsia spongiae sp. nov., an actinomycete isolated from the marine sponge Amphimedon viridis

Authors: Cláudia Beatriz Afonso de Menezes^1,2 , Rafael Sanches Afonso¹ , Wallace Rafael de Souza³ , Márcia Parma³ , Itamar Soares de Melo³ , Tiago Domingues Zucchi^3,4 , Fabiana Fantinatti-Garboggini^1,2
- VIEW AFFILIATIONS
- ¹ 1Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Av. Alexandre Cazelatto 999, CEP 13148-218, Paulínia, SP, Brazil ² 2Programa de Pós-Graduação em Genética e Biologia Molecular, UNICAMP, CP 6109, 13083-970, Campinas, SP, Brazil ³ 3Embrapa Meio Ambiente, Rodovia SP 340 Km 127.5, CP 69, CEP 13820-000, Jaguariúna, SP, Brazil ⁴ 4Agrivalle – Biotecnologia Agrícola, Rua Moisés Lopes Silva 50, CEP 37550-000, Pouso Alegre, MG, Brazil
*Correspondence: Fabiana Fantinatti-Garboggini, [email protected]
First Published Online: 30 May 2017, International Journal of Systematic and Evolutionary Microbiology 67: 1260-1265, doi: 10.1099/ijsem.0.001796
Subject: New Taxa - Actinobacteria

Received: 05/06/2016
Accepted: 13/01/2017
Cover date: 01/05/2017

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A novel actinobacterium, designated isolate B138T, was isolated from the marine sponge, Amphimedon viridis, which was collected from Praia Guaecá (São Paulo, Brazil), and its taxonomic position was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Williamsia and it formed a distinct phyletic line in the Williamsia 16S rRNA gene tree. It was most closely related to Williamsia serinedens DSM 45037T and Williamsia deligens DSM 44902T (99.0 % 16S rRNA gene sequence similarity) and Williamsia maris DSM 44693T (97.5 % 16S rRNA gene sequence similarity), but was distinguished readily from these strains by the low DNA–DNA relatedness values (62.3–64.4 %) and by the discriminatory phenotypic properties. Based on the data obtained, the isolate B138T (=CBMAI 1094T=DSM 46676T) should be classified as the type strain of a novel species of the genus Williamsia , for which the name Williamsia spongiae sp. nov. is proposed.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA sequence of Williamsia spongiae B138T (=CBMAI 1094T =DSM 46676T) is JN615440.
Four supplementary figures are available with the online Supplementary Material.

Keyword(s): marine sponge, Polyphasic taxonomy, São Paulo state, Williamsia spongiae

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