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- Volume 67, Issue 8
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f Leucobacter ruminantium sp. nov., isolated from the bovine rumen
- Authors: Byung Hee Chun1 , Hyo Jung Lee1,2 , Sang Eun Jeong1 , Peter Schumann3 , Che Ok Jeon1
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1 1Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea 2 2Department of Biology, Kunsan National University, Gunsan 54150, Republic of Korea 3 3Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, D-38124 Braunschweig, Germany
- *Correspondence: Che Ok Jeon, [email protected]
- First Published Online: 10 August 2017, International Journal of Systematic and Evolutionary Microbiology 67: 2634-2639, doi: 10.1099/ijsem.0.002003
- Subject: New Taxa - Actinobacteria
- Received:
- Accepted:
- Cover date:




Leucobacter ruminantium sp. nov., isolated from the bovine rumen, Page 1 of 1
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A Gram-stain-positive, lemon yellow-pigmented, non-motile, rod-shaped bacterium, designated strain A2T, was isolated from the rumen of cow. Cells were catalase-positive and weakly oxidase-positive. Growth of strain A2T was observed at 25–45 °C (optimum, 37–40 °C), at pH 5.5–9.5 (optimum, pH 7.5) and in the presence of 0–3.5 % (w/v) NaCl (optimum, 1 %). Strain A2T contained iso-C16 : 0 and anteiso-C15 : 0 as the major cellular fatty acids. Menaquinone-11 was detected as the sole respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2T formed a distinct phyletic lineage within the genus Leucobacter . Strain A2T was most closely related to ‘ Leucobacter margaritiformis ’ A23 (97.7 % 16S rRNA gene sequence similarity) and Leucobacter tardus K 70/01T (97.2 %). The major polar lipids of strain A2T were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. Strain A2T contained a B-type cross-linked peptidoglycan based on 2,4-diaminobutyric acid as the diagnostic diamino acid with threonine, glycine, alanine and glutamic acid but lacking 4-aminobutyric acid. The G+C content of the genomic DNA was 67.0 %. From the phenotypic, chemotaxonomic and molecular features, strain A2T was considered to represent a novel species of the genus Leucobacter , for which the name Leucobacter ruminantium sp. nov. is proposed. The type strain is A2T (=KACC 17571T=JCM 19316T).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A2T is KC107829.
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Three supplementary figures and one supplementary table are avialable with the online Supplementary Material.
- Keyword(s): bovine rumen, Leucobacter ruminantium, actinobacteria, new taxa
© 2017 IUMS | Published by the Microbiology Society
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