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f Desulfocucumis palustris gen. nov., sp. nov., a mesophilic sulfate reducer belonging to Desulfotomaculum subcluster Ig

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  • Authors: Miho Watanabe1​,2​ ,  Hisaya Kojima1​ , Manabu Fukui1​
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    • 1​ 1​The Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8, Kita-ku, Sapporo 060-0819, Japan 2​ 2​Postdoctoral Research Fellow of the Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo 102-8471, Japan
  • *Correspondence: Miho Watanabe, [email protected]
  • First Published Online: 08 August 2017, International Journal of Systematic and Evolutionary Microbiology 67: 2679-2682, doi: 10.1099/ijsem.0.002005
  • Subject: New Taxa - Firmicutes and Related Organisms
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Desulfocucumis palustris gen. nov., sp. nov., a mesophilic sulfate reducer belonging to Desulfotomaculum subcluster Ig, Page 1 of 1

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  • A mesophilic, endospore-forming, sulfate-reducing bacterium, designated strain NAW-5, was isolated from marsh soil. Cells of strain NAW-5 were Gram-stain-negative, curved rods that were motile. Strain NAW-5 grew at 18–48 °C (optimum 32–37 °C) and pH 5.8–8.4 (optimum pH 6.2–7.3). Electron donors utilized were various organic acids and H2 which support autotrophic growth. Fermentative growth occurred on carboxylic acids, but not on sugar. Sulfate, thiosulfate and elemental sulfur were used as electron acceptors. The respiratory isoprenoid quinone was MK-7. The genomic DNA G+C content of this strain was 46.6 mol%. Sequence analysis of the 16S rRNA gene showed that strain NAW-5 was affiliated to the family ‘Desulfotomaculaceae’ but the strain shared very low sequence similarity with any representatives of this family (≥89 %). Strain NAW-5 belongs to Desulfotomaculum subcluster Ig which does not include any species with validly published names. On the basis of significant differences in the phylogenetic and phenotypic properties between strain NAW-5 and related species, strain NAW-5 represents a novel species of a new genus for which the name Desulfocucumis palustris gen. nov., sp. nov. is proposed. The type strain of the type species is NAW-5 (=DSM 102911=NBRC 112242).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NAW-5 is LC183909.

  • Two supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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