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f Sphingomonas crusticola sp. nov., isolated from biological soil crusts

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  • Authors: Kaishuai Zhang1​ ,  Kai Tang1​ ,  Fuying Feng1​ ,  Bo Yuan2​ ,  Xiaojun Zhang3​ , Jianyu Meng1​
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    • 1​ 1​Institute for Applied and Environmental Microbiology, College of Life Science, Inner Mongolia Agricultural University, Huhhot 010018, PR China 2​ 2​College of Life Science, Inner Mongolia Normal University, Huhhot 010018, PR China 3​ 3​Jining District Animal Disease Prevention and Control Center, Jining 012000, PR China
  • *Correspondence: Jianyu Meng, [email protected]
  • First Published Online: 18 August 2017, International Journal of Systematic and Evolutionary Microbiology 67: 3033-3037, doi: 10.1099/ijsem.0.002074
  • Subject: New Taxa - Proteobacteria
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  • A yellow-pigmented, Gram-stain-negative, short-rod-shaped bacterial strain, MIMD3, was isolated from biological soil crusts collected in Liangcheng, north-western China. Cell growth could be observed at 10–37 °C (optimum 25 °C), at pH 5–8 (optimum 6.6) and in the presence of 1 % (w/v) NaCl (optimum 0 %). The genomic DNA G+C content was 65.0 mol%. Analysis of 16S rRNA gene sequences showed that strain MIMD3 shared the highest similarity with Sphingomonas vulcanisoli KCTC 42454 (95.1 %), Sphingomonas oligophenolica JCM 12082 (94.8 %), Sphingomonas mali IFO 15500 (94.5 %), Sphingomonas . leidyi ATCC 15260 (94.4 %) and Sphingomonas formosensis CC-Nfb-2 (94.3 %). The strain had Q-10 as the predominant respiratory quinone, and sym-homospermidine as the major polyamine. The major fatty acids of the strain were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C19 : 0 cyclo ω8c, C14 : 0 2-OH and C16 : 0. The main polar lipids of strain MIMD3 were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, it is concluded that strain MIMD3 represents a novel species of the genus Sphingomonas , for which the name Sphingomonas crusticola sp. nov. is proposed. The type strain is MIMD3 (=KCTC 42801=MCCC 1K01310).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MIMD3 is KT346426.

  • Four supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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2019-02-17
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