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Volume 67, Issue 9

sp. nov., isolated from soil in Thailand Free

Abstract

A thermophilic poly(L-lactide)-degrading Gram-stain-positive filamentous bacterial strain that develops single spores on the aerial mycelium was isolated from forest soil at Srinagarind Dam, Kanchanaburi Province, Thailand. The results of a polyphasic taxonomic study showed that our isolate had characteristics typical of members of the genus . The isolate grew aerobically at an optimum temperature of 50–55 °C and optimal pH 6–7. -diaminopimelic acid was present as the diagnostic diamino acid in the peptidoglycan but no characteristic sugars are detected. The predominant menaquinone was MK–7. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine diphosphatidylglycerol, phosphatidylglycerol and phosphatidylserine. The predominant cellular fatty acid was iso–C. The DNA G+C content of strain KSR 13 was 53.4 mol%. The 16S rRNA gene sequence analysis also indicated that strain KSR 13 belonged to the genus , being most closely related to JIR-001 (99.2 %). The DNA–DNA relatedness values that distinguished KSR 13 from JIR-001 were 17.8–32.1 %, which were significantly below the 70 % cutoff value recommended for species delineation. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, the new isolate is proposed as a novel species and named sp. nov. The type strain is KSR 13 (=BCC 50740=NBRC 109332).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Firmicutes , PLA-degrading bacteria , Polycladomyces subterraneus and thermophilic filamentous bacteria
© 2017 IUMS
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2017-09-01
2024-04-26
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References

  1. Tsubouchi T, Shimane Y, Mori K, Usui K, Hiraki T et al. Polycladomyces abyssicola gen. nov., sp. nov., a thermophilic filamentous bacterium isolated from hemipelagic sediment. Int J Syst Evol Microbiol 2013; 63:1972–1981 [View Article][PubMed]
     [Google Scholar]
  2. Sukkhum S, Tokuyama S, Tamura T, Kitpreechavanich V. A novel poly (L-lactide) degrading actinomycetes isolated from Thai forest soil, phylogenic relationship and the enzyme characterization. J Gen Appl Microbiol 2009; 55:459–467 [View Article][PubMed]
     [Google Scholar]
  3. Itoh T, Kudo T, Parenti F, Seino A. Amended description of the Genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 1989; 39:168–173 [View Article]
     [Google Scholar]
  4. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
     [Google Scholar]
  5. Cappuccino JG, Sherman N. Microbiology – A Laboratory Manual, 7th ed. NY: Pearson Education; 2007
     [Google Scholar]
  6. Arai T. Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan; 1975
     [Google Scholar]
  7. Williams ST, Cross T. Actinomycetes. In Booth C. (editor) Methods in Microbiology vol. 4 London: Academic Press; 1971 pp. 295–334
     [Google Scholar]
  8. Greenwood JR, Pickett MJ. Salient features of Haemophilus vaginalis. J Clin Microbiol 1979; 9:200–204[PubMed]
     [Google Scholar]
  9. Reasoner DJ, Geldreich EE. A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 1985; 49:1–7[PubMed]
     [Google Scholar]
  10. Tamura T, Nakagaito Y, Nishii T, Hasegawa T, Stackebrandt E et al. A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 1994; 44:193–203 [View Article][PubMed]
     [Google Scholar]
  11. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974; 28:226–23[PubMed]
     [Google Scholar]
  12. Komagata K, Suzuki K I. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
     [Google Scholar]
  13. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  14. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI, Inc; 1990
     [Google Scholar]
  15. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996; 42:989–1005 [View Article]
     [Google Scholar]
  16. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  17. Wu C, Lu X, Qin M, Wang Y, Ruan J. Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English Translation of Mikrobiologiia] 1989; 16:176–178
     [Google Scholar]
  18. Saito H, Miura KI. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 1963; 72:619–629 [View Article][PubMed]
     [Google Scholar]
  19. Kawasaki H, Hoshino Y, Hirata A, Yamasato K. Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among phototrophic purple nonsulfur bacteria. Arch Microbiol 1993; 160:358–362 [View Article][PubMed]
     [Google Scholar]
  20. Yamada Y, Katsura K, Kawasaki H, Widyastuti Y, Saono S et al. Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the α-Proteobacteria. Int J Syst Evol Microbiol 2000; 50:823–829 [View Article][PubMed]
     [Google Scholar]
  21. Katsura K, Kawasaki H, Potacharoen W, Saono S, Seki T et al. Asaia siamensis sp. nov., an acetic acid bacterium in the α-Proteobacteria. Int J Syst Evol Microbiol 2001; 51:559–563 [View Article][PubMed]
     [Google Scholar]
  22. Suriyachadkun C, Chunhametha S, Thawai C, Tamura T, Potacharoen W et al. Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 2009; 59:992–997 [View Article][PubMed]
     [Google Scholar]
  23. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  24. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  25. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  26. Felsenstein J. Parsimony in systematics: biological and statistical issues. Annu Rev Ecol Syst 1983; 14:313–333 [View Article]
     [Google Scholar]
  27. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  28. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  29. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
     [Google Scholar]
  30. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
     [Google Scholar]
  31. Verlander CP. Detection of horseradish peroxidase by colorimetry. In Kricka LJ. (editor) Nonisotopic DNA Probe Techniques NY: Academic Press; 1992 pp. 185–201 [CrossRef]
     [Google Scholar]
  32. Wayne LG, Brenner DJ, Colwell RR, Grimon PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [CrossRef]
     [Google Scholar]
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Polycladomyces subterraneus sp. nov., isolated from soil in Thailand
Int J Syst Evol Microbiol 67, 3323 (2017); https://doi.org/10.1099/ijsem.0.002114
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