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f Swionibacillus sediminis gen. nov., sp. nov., a member of the family Bacillaceae isolated from ocean sediment

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Swionibacillus sediminis gen. nov., sp. nov., a member of the family Bacillaceae isolated from ocean sediment, Page 1 of 1

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  • A Gram-stain-negative, non-motile, strictly aerobic bacterium designated BW11-2 was isolated from marine sediment of the south-west Indian Ocean. Cells of BW11-2 were rod-shaped, endospore-forming, 0.3–0.5 µm wide, 1.8–2.0 µm long, catalase-positive and oxidase-negative. The isolate was capable of growing at 15–45 °C (optimum 30 °C), pH 5–9 (optimum 7) and with 0.5–10 % (w/v) NaCl (optimum 3 %). Based on 16S rRNA gene sequence similarities, BW11-2 was shown to belong to the family Bacillaceae within the phylum Firmicutes and formed a distinct lineage, showing the highest sequence similarities to closely related genera: Bacillus (93.9–94.7 %), Graci libacillus (93.3–93.7 %), Amphibacillus (93.5 %), Virgibacillu s (92.9–93.1 %) and Anaerobacillus (92.6–93.0 %). BW11-2 shared the highest 16S rRNA gene sequence similarity with the species Bacillus oleronius (94.7 %). The predominant fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0. The major quinone was menaquinone-7 (MK-7). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified aminolipid. The genomic DNA G+C content of strain BW11-2 was 43.3 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain BW11-2 represents a novel genus and species in the family Bacillaceae , for which the name Swionibacillus sediminis gen. nov., sp. nov. is proposed. Strain BW11-2 (=CICC 24196=JCM 31924) is the type strain.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BW11-2 is KY635836.

  • Three supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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2017-08-31
2019-02-24
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