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f Paenibacillus albidus sp. nov., isolated from grassland soil

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  • Authors: Junli Zhuang1​ ,  Di Xin1​ ,  Yu-Qin Zhang2​ ,  Jingnan Guo1​ , Jianli Zhang1​
    • VIEW AFFILIATIONS
    • 1​ 1​School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China 2​ 2​Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China
  • *Correspondence: Jianli Zhang [email protected]
  • First Published Online: 27 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4685-4691, doi: 10.1099/ijsem.0.002356
  • Subject: New taxa - Firmicutes and Related Organisms
  • Received:
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  • A novel bacterial strain, designed Q4-3, was isolated from a soil sample obtained from Qilian grassland, Qinghai, China. Phylogenetic, phenotypic, chemotaxonomic and molecular analyses were performed on the new isolate. Cells were Gram-stain-positive, facultatively anaerobic, spore-forming, motile rods with peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences placed strain Q4-3 in the genus Paenibacillus, and its closest relatives were Paenibacillus odorifer JCM 21743, Paenibacillus typhae DSM 25190, Paenibacillus borealis DSM 13188 and Paenibacillus etheri DSM 29760 with 16S rRNA gene sequence similarities of 98.12, 97.89, 97.63 and 97.6 %, respectively. The isolate grew at 4-37 °C (optimum 28-30 °C), at pH 6.0-10.0 (optimum pH 7.5) and with 0-3 %(w/v) NaCl (optimum 1 %). The DNA of strain Q4-3 was determined to be 48.6 mol%. The predominant menaquinone was MK-7 and the diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid. Anteiso-C15 : 0 (55.5 %), iso-C16 : 0 (14.5 %) and C16 : 0 (13.3 %) were the major fatty acids. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified lipid. Based on these results, strain Q4-3 is considered to represent a novel of the genus Paenibacillus, for which the name Paenibacillus albidus nov. is proposed. The type strain is Q4-3 (=CGMCC 1.16134=KCTC 33911).

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and nifH sequences of strain Q4-3 are KY674517 and KY800539, respectively.

  • Six supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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