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- Volume 67, Issue 11
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f Ciceribacter thiooxidans sp. nov., a novel nitrate-reducing thiosulfate-oxidizing bacterium isolated from sulfide-rich anoxic sediment
- Authors: Tongchu Deng1,2,3,4 , Xingjuan Chen1,2 , Qin Zhang1,2 , Yuming Zhong1,2 , Jun Guo1,2,3 , Guoping Sun1,2,3 , Meiying Xu1,2
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- VIEW AFFILIATIONS
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1 1Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, PR China 2 2State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China 3 3South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China 4 4University of Chinese Academy of Sciences, Beijing 100049, PR China
- *Correspondence: Meiying Xu [email protected]
- First Published Online: 06 October 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4710-4715, doi: 10.1099/ijsem.0.002367
- Subject: New taxa - Proteobacteria
- Received:
- Accepted:
- Cover date:




Ciceribacter thiooxidans sp. nov., a novel nitrate-reducing thiosulfate-oxidizing bacterium isolated from sulfide-rich anoxic sediment, Page 1 of 1
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Two facultative chemolithotrophic, nitrate-reducing thiosulfate-oxidizing strains, F43bT and F21, were isolated from the sulfide-rich anoxic sediment of an urban creek in Pearl River Delta, China. Both strains were Gram-negative, facultatively anaerobic, non-spore-forming and rod-shaped with a flagellum. Phylogenetic analyses of 16S rRNA genes and the thrC, recA, glnII and atpD housekeeping genes revealed that the type strain shared high sequence similarities to Ciceribacter lividus MSSRFBL1T, with 98.8, 90.9, 94.8, 95.4 and 96.1 % identity, respectively. In addition, the major isoprenoid quinone (ubiquinone Q-10) and the DNA G+C content (66.0 mol%) of the type strain were similar to those of Ciceribacter lividus MSSRFBL1T. These results strongly support the classification of strains F43bT and F21 into the genus Ciceribacter . However, these strains diverged markedly from strain MSSRFBL1T with respect to several physiological and biochemical properties such as their semi-translucent colonies and nitrate-reducing and simultaneous thiosulfate-oxidizing respiration. Furthermore, the predominant fatty acids of strain F43bT were summed feature 2 (C18 : 1ω9t and/or C18 : 1ω9c and/or C18 : 1ω11t), C14 : 0 3-OH, C18 : 0 and C16 : 0, and its polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidymonomethylethanolamine and an unidentified glycolipid, which represented another two significant differences from strain MSSRFBL1T. Importantly, the DNA–DNA relatedness between strain F43bT and MSSRFBL1T was only 47.7 %. Based on the aforementioned polyphasic taxonomic results, the two isolates are suggested to represent a novel species of the genus Ciceribacter , for which the name Ciceribacter thiooxidans sp. nov. is proposed; the type strain is F43bT (=CCTCC AB 2016062T=KCTC 52231T).
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The GenBank accession numbers for 16S rRNA gene sequences of strains F43bT and F21 are KU975391 and KU985322, respectively. Accession numbers for the thrC gene sequences of strains F43bT , F21, Rhizobium rosettiformans W3T and Rhizobium daejeonense L61T are KY283819, KY283820, KY290754 and KY290755, respectively. Accession numbers for the recA gene sequences of strains Rhizobium rosettiformans W3T , Rhizobium daejeonense L61T , Rhizobium skierniewicense Ch11T , F43bT and F21 are MF537311–MF537315, respectively. Accession numbers for the glnII gene sequences of strains F43bT , F21, Ciceribacter lividus MSSRFBL1T , Rhizobium rosettiformans W3T and Rhizobium daejeonense L61T are KY283818 and KX131137–KX131140, respectively. Accession numbers for the atpD gene sequences of e sequences of strains F43bT and F21 are KX131135 and KX131136, respectively.
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Nine supplementary figures are available with the online Supplementary Material.
- Keyword(s): phylogenetic analysis, nitrate-reducing thiosulfate-oxidizing bacterium, Ciceribacter, sulfide-rich anoxic sediment, novel species
© 2017 IUMS | Published by the Microbiology Society
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