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Volume 68, Issue 3

sp. nov., isolated from tidal flat sediment Free

Abstract

A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea. Strain CJ11 grew optimally on R2A at 30 °C and pH 7.0. Sequencing results of the 16S rRNA gene revealed that strain CJ11 possesses two copies of the 16S rRNA gene varying at five nucleotide positions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11 belonged to the genus within the family and was most closely related to KCTC 33691 (99.36–99.15 % similarity). DNA–DNA relatedness levels of strain CJ11 was 41.7 % (reciprocal, 57.8 %) to KCTC 33691. The G+C content of the genomic DNA was 51.0 mol%. Strain CJ11contained -diaminopimelic acid in the cell-wall peptidoglycan. The major isoprenoid quinone was menaquinone-7. The major cellular fatty acids were anteiso-C, C and iso-C. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids. On the basis of the polyphasic taxonomic study, strain CJ11 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CJ11 (=KACC 19304=JCM 32080).

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Keyword(s): 16S rRNA gene heterogeneity , Paenibacillus and tidal flat sediment
© 2018 IUMS
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2018-03-01
2024-04-26
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Paenibacillus translucens sp. nov., isolated from tidal flat sediment
Int J Syst Evol Microbiol 68, 936 (2018); https://doi.org/10.1099/ijsem.0.002613
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