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- Volume 68, Issue 4
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f Microbacterium telephonicum sp. nov., isolated from the screen of a cellular phone
- Authors: Praveen Rahi1 , Rashmi Kurli1 , Aabeejjeet N. Pansare1 , Mitesh Khairnar1 , Shubhangi Jagtap1 , Nisha B. Patel2 , Syed G. Dastager3 , Paul A. Lawson2 , Yogesh S. Shouche1
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- VIEW AFFILIATIONS
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1 1National Centre for Microbial Resource, National Centre for Cell Science, Pune, Maharashtra 411007, India 2 2Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA 3 3NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune, Maharashtra 411008, India
- *Correspondence: Praveen Rahi [email protected]
- First Published Online: 09 February 2018, International Journal of Systematic and Evolutionary Microbiology 68: 1052-1058, doi: 10.1099/ijsem.0.002622
- Subject: New Taxa - Actinobacteria
- Received:
- Accepted:
- Cover date:




Microbacterium telephonicum sp. nov., isolated from the screen of a cellular phone, Page 1 of 1
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A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T, exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium , and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T. The DNA–DNA relatedness values of the strain S2T63T to M. aurantiacum KACC 20510T, M. kitamiense KACC 20514Tand Microbacterium laevaniformans KACC 14463T were 65 % (±4), 29.5 % (±3) and 55.9 % (±4), respectively. The genomic DNA G+C content was 71.8 mol%. The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids. The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1α. The predominant menaquinones were MK-12 and MK-13. Rhamnose, fucose and galactose were the whole-cell sugars detected. The strain also showed biofilm production, estimated by using crystal violet assay. Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium , for which the name Microbacteriumtelephonicum is proposed, with S2T63T (=MCC 2967T=KACC 18715T=LMG 29293T) as the type strain.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain S2T63T is KT735048.
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Two supplementary tables and five supplementary figures are available with the online version of this article.
- Keyword(s): mobile phone, MALDI-TOF MS, bio-film, Actinobacteria
© 2018 IUMS | Published by the Microbiology Society
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