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- Volume 68, Issue 4
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f Halomonas saliphila sp. nov., a moderately halophilic bacterium isolated from a saline soil
- Authors: Longzhan Gan1 , Xiufeng Long1 , Heming Zhang1 , Yanyan Hou1 , Jiewei Tian1 , Yuqin Zhang2 , Yongqiang Tian1
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- VIEW AFFILIATIONS
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1 1Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China 2 2Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and PeKing Union Medical College, Beijing 100050, PR China
- *Correspondence: Yongqiang Tian [email protected]
- First Published Online: 19 February 2018, International Journal of Systematic and Evolutionary Microbiology 68: 1153-1159, doi: 10.1099/ijsem.0.002644
- Subject: New Taxa - Proteobacteria
- Received:
- Accepted:
- Cover date:




Halomonas saliphila sp. nov., a moderately halophilic bacterium isolated from a saline soil, Page 1 of 1
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A novel, Gram-stain-negative, aerobic, rod-shaped, motile and moderately halophilic bacterium, designated strain LCB169T, was isolated from a saline soil sample from Gansu Province, PR China. The cells of LCB169T grew at 10–52 °C (optimum 30 °C), at pH 6.0–10.0 (optimum pH 8.0) and in the presence of 0–17 % (w/v) NaCl (optimum 10–15 %). Phylogenetic analysis based on 16S rRNA gene sequences and concatenated 16S rRNA, gyrB and rpoD genes sequences revealed that LCB169T represented a member of the genus Halomonas in the class Gammaproteobacteria . The most closely related species were Halomonas daqingensis DQD2-30T (98.0 % 16S rRNA gene sequence similarity), Halomonas kenyensis AIR-2T (97.8 %) and Halomonas desiderata FB2T (97.5 %). DNA–DNA relatedness values between LCB169T and H. daqingensis CGMCC 1.6443T, H. desiderata DSM 9502T and H. kenyensis DSM 17331T were 33, 35 and 38 %, respectively. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and three unidentified phospholipids. The major fatty acids were C18 : 1ω7c, C16 : 0, C16 : 1ω7c and C12 : 0 3-OH. The genomic DNA G+C content was 66.1 mol% and the predominant respiratory quinone was Q-9. On the basis of the results of phenotypic, phylogenetic and chemotaxonomic analyses and DNA–DNA hybridization relatedness values, LCB169T is considered to represent a novel species of the genus Halomonas, for which the name Halomonas saliphila sp. nov. is proposed. The type strain is LCB169T (=CGMCC 1.15818T=KCTC 52618T).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LCB169T is KX008964. The accession numbers for the gyrB gene sequences of strain LCB169T, Halomonas daqingensis DQD2-30T and H. kenyensis AIR-2T are KY971522, MF919314 and MF919315, and those for the rpoD gene sequences are KY971523, MF919316 and MF919317, respectively.
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Three supplementary figures are available with the online version of this article.
- Keyword(s): novel species, halophilic, Halomonas saliphila, saline soil
© 2018 IUMS | Published by the Microbiology Society
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