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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 6

Specibacter cremeus gen. nov., sp. nov., a new member of the family Micrococcaceae isolated from a natural cave Free

Abstract

A Gram-reaction-positive, non-spore-forming, rod-shaped bacterium, strain C1-50, was isolated from a natural cave in Jeju, Republic of Korea by using the serial dilution plating method. Results of phylogenetic analysis using 16S rRNA gene sequences showed that strain C1-50 belonged to the family Micrococcaceae but had the highest sequence similarity to Arthrobacter halodurans JSM 078085 (96.18 %) and Arthrobacter globiformis DSM 20124 (96.04 %). The 16S rRNA gene sequence similarities between strain C1-50 and other members of the family were lower than 96.0 %. The cell-wall peptidoglycan type was A3α with an l-Lys-l-Ala2. Whole-cell sugars consisted largely of glucose and galactose. The predominant menaquinone was MK-9(H2) with smaller components of MK-7(H2) and MK-8(H2). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The cellular fatty acids consisted of saturated, unsaturated, anteiso-branched and iso-branched components. The G+C content of genomic DNA was 68.8 mol% (draft genome sequence). On the basis of morphological and chemotaxonomic differences and distinct phylogenetic clustering, it was concluded that the organism represents a novel species of a new genus in the family Micrococcaceae , for which the name Specibacter cremeus gen. nov., sp. nov. is proposed. The type strain is C1-50 (=KCTC 39557=DSM 100066).

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Keyword(s): 16S rRNA gene , cave , draft genome sequence , Micrococcaceae , polyphasic approach and Specibacter cremeus gen. nov., sp. nov.
© 2019 IUMS
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2019-04-16
2024-04-26
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Specibacter cremeus gen. nov., sp. nov., a new member of the family Micrococcaceae isolated from a natural cave
Int J Syst Evol Microbiol 69, 1767 (2019); https://doi.org/10.1099/ijsem.0.003392
/content/journal/ijsem/10.1099/ijsem.0.003392
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