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Volume 69, Issue 12

sp. nov., isolated from lakewater Free

Abstract

A novel Gram-stain-negative, strictly aerobic, rod-shaped, gray-pigmented bacterial strain, designated L-25–5 w-1, was isolated from the water at Baiyang Lake, PR China. Cells of strain L-25–5 w-1 were motile, with a single polar flagellum. L-25–5 w-1 was able to grow at 15–37 °C (optimum 37 °C) and pH 5–8 (optimum pH 6) in R2A medium. 16S rRNA gene sequence analysis and phylogenetic analysis of L-25–5 w-1 showed the highest relationship to GSF71 (97.9 %), DSM 21654 (97.9 %) and CC-HIH038 (97.0 %), with other species of the genus showing less than 97 % sequence similarity. The predominant polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminolipids; the major cellular fatty acids were C, C 3-OH, C 2-OH, iso-C, summed feature 2 (C aldehyde and/or unknown 10.9525 and/or iso-CI and/or C 3-OH), summed feature 3 (Cω7 and/or Cω6) and summed feature 8 (Cω7 and/or Cω6); and the major respiratory quinone was ubiquinone 10. The draft genome size of L-25–5 w-1 was 5.8 Mbp, and the DNA G+C content was 66.6 %. The average nucleotide identity value and digital DNA–DNA hybridization relatedness value between L-25–5 w-1 and related type strains were 80.2 and 24.7 % with GSF71 and 78.8 and 23.6 % with DSM 21654, respectively. According to the phylogenetic, chemotaxonomic and genotypic properties, strain L-25–5 w-1 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is L-25–5 w-1 (=CGMCC 1.13672=KCTC 62777).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Azospirillum griseum sp. nov. , Baiyang Lake and freshwater
© 2019 The Authors
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2019-12-01
2024-04-26
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Azospirillum griseum sp. nov., isolated from lakewater
Int J Syst Evol Microbiol 69, 3676 (2019); https://doi.org/10.1099/ijsem.0.003460
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