1887

Abstract

Ethanol production at high temperatures has garnered much interest in recent years and a key factor is the availability of thermotolerant yeasts. During an investigation on the diversity of thermotolerant yeasts from different habitats, a novel yeast species from the spent wash of a distillery unit associated with a sugar factory was isolated. Phylogenetic analysis of D1/D2 large subunit and ITS rRNA genes placed this species in the ascomycetous genus . The novel species can be distinguished from the closely related species using these rRNA gene regions. The cells of the new species are ovoid to ellipsoid with a diameter of 3.5–6.0×2.4–3.10 µm, while cells are cylindrical with a cell diameter of 1.5–3.0×6–23 µm. This novel species represents, together with , one of the two most thermotolerant yeast species in the genus , able to grow at 42 °C sp. nov. is proposed during this study.

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2019-10-01
2024-05-04
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References

  1. Lachance MA, Kurtzman CP. Wickerhamiella van der Walt (1973). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp. 891–897
    [Google Scholar]
  2. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
    [Google Scholar]
  3. Vu D, Groenewald M, Szöke S, Cardinali G, Eberhardt U et al. DNA barcoding analysis of more than 9000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 2016; 85:91–105 [View Article][PubMed]
    [Google Scholar]
  4. Badotti F, Silva PA, Mendonça MC, Gomes FC, Morais PB et al. Wickerhamiella dulcicola sp. nov. and Wickerhamiella cachassae sp. nov., yeasts isolated from cachaça fermentation in Brazil. Int J Syst Evol Microbiol 2013; 63:1169–1173 [View Article][PubMed]
    [Google Scholar]
  5. de Vega C, Albaladejo RG, Guzmán B, Steenhuisen S–L, Johnson SD et al. Flowers as a reservoir of yeast diversity: description of Wickerhamiella nectarea f.a. sp. nov., and Wickerhamiella natalensis f.a. sp. nov. from South African flowers and pollinators, and transfer of related Candida species to the genus Wickerhamiella as new combinations. FEMS Yeast Res 2017; 17:fox 054 [View Article]
    [Google Scholar]
  6. Barbosa AC, Morais CG, Morais PB, Rosa LH, Pimenta RS et al. Wickerhamiella pagnoccae sp. nov. and Candida tocantinsensis sp. nov., two ascomycetous yeasts from flower bracts of Heliconia psittacorum (Heliconiaceae). Int J Syst Evol Microbiol 2012; 62:459–464 [View Article][PubMed]
    [Google Scholar]
  7. Lachance MA, Vale HMM, Sperandio EM, Carvalho AOS, Santos ARO et al. Wickerhamiella dianesei f.a., sp. nov. and Wickerhamiella kurtzmanii f.a., sp. nov., two yeast species isolated from plants and insects. Int J Syst Evol Microbiol 2018; 68:3351–3355 [View Article][PubMed]
    [Google Scholar]
  8. Nakase T, Jindamorakot S, Limtong S, Am-In S, Kawasaki H et al. Candida kazuoi sp. nov. and Candida hasegawae sp. nov., two new species of ascomycetous anamorphic yeasts isolated from insect frass in Thailand. J Gen Appl Microbiol 2007; 53:239–245 [View Article][PubMed]
    [Google Scholar]
  9. Hagler AN, Ribeiro JR, Pinotti T, Brandão LR, Pimenta RS et al. Wickerhamiella slavikovae sp. nov. and Wickerhamiella goesii sp. nov., two yeast species isolated from natural substrates. Int J Syst Evol Microbiol 2013; 63:3099–3103 [View Article][PubMed]
    [Google Scholar]
  10. Nakase T, Komagata K, Fukazawa Y. Candida pararugosa, a new species of asporogenous yeast. J Gen Appl Microbiol 1978; 24:17–25 [View Article]
    [Google Scholar]
  11. Lachance MA, Rosa CA, Starmer WT, Schlag-Edler B, Barker JS et al. Wickerhamiella australiensis, Wickerhamiella cacticola, Wickerhamiella occidentalis, Candida drosophilae and Candida lipophila, five new related yeast species from flowers and associated insects. Int J Syst Bacteriol 1998; 48:1431–1443 [View Article][PubMed]
    [Google Scholar]
  12. Yarrow D. Methods for the isolation, maintenance and identification of yeasts. In The Yeasts 2008
    [Google Scholar]
  13. Barnett JA, Payne RW & YD. Yeast, characteristics and identification vol. 3 Cambridge Univ Press; 2000 pp. 1150
    [Google Scholar]
  14. Aamir S, Sutar S, Singh SK, Baghela A. A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. Plant Pathol Quar 2015; 5:74–81 [View Article]
    [Google Scholar]
  15. Kurtzman CP, Robnett CJ. Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. FEMS Yeast Res 2003; 3:417–432 [View Article][PubMed]
    [Google Scholar]
  16. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
    [Google Scholar]
  17. Tamura K. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+ C-content biases. Mol Biol Evol 1992; 9:678–687
    [Google Scholar]
  18. van der Walt JP, Liebenberg N. The yeast genus Wickerhamiella gen. nov. (Ascomycetes). Antonie van Leeuwenhoek Int J Gen Mol Microbiol 1973; 1;39:121–128
    [Google Scholar]
  19. Patil C, Kalburgi PB & GM. Performance and evaluation of sugar & distillery effluent treatment plant. Int Res J Eng Technol 2015; 02:1456–1460
    [Google Scholar]
  20. Murata Y, Danjarean H, Fujimoto K, Kosugi A, Arai T et al. Ethanol fermentation by the thermotolerant yeast, Kluyveromyces marxianus TISTR5925, of extracted sap from old oil palm trunk. Aims Energy 2015; 10;3:201–203
    [Google Scholar]
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