1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 12

sp. nov., isolated from rhizosphere soil of Free

Abstract

A yellow-pigmented bacterial strain, designated T13, was isolated from the rhizosphere soil of collected from Xinjiang, PR China. Cells were rod-shaped, Gram-stain-negative, aerobic and gliding. Strain T13 grew optimally at 25–30 °C and pH 7.0–8.0 with a NaCl tolerance of 0–2 % on Reasoner's 2A agar. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain T13 belonged to the genus within the family and was closely related to KCTC 42884 with a similarity value of 97.4 %. The major polar lipid was phosphatidylethanolamine; the only respiratory quinone was MK-6, and the polyamine profile contained -homospermidine as the major polyamine and a trace amount of spermidine. The major fatty acids were iso-C, ω7 and summed feature 3 (comprising Cω7 or Cω6). The G+C content of the genomic DNA was 34.1 mol%. It is concluded from the phenotypic and genotypic data that strain T13 represents a novel species of the genus , for which the name sp. nov. with the type strain T13 (=KCTC 62874=ACCC 60126) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Alhagi sparsifolia and Bacteroidetes
© 2019 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003717
2019-12-01
2024-05-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/12/3955.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003717&mimeType=html&fmt=ahah

References

  1. Bergey D, Harrison F, Breed R, Hammer B, Huntoon F et al. Genus II. Flavobacterium gen. nov. Bergey’s Manual of Determinative Bacteriology 1923 pp. 97–117
     [Google Scholar]
  2. Bernardet J-F, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 1996; 46:128–148 [View Article]
     [Google Scholar]
  3. Dong K, Chen F, du Y, Wang G. Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense . Int J Syst Evol Microbiol 2013; 63:886–892 [View Article][PubMed]
     [Google Scholar]
  4. Bernardet JF, Bowman JP. Flavobacterium. Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Ltd; 2015
     [Google Scholar]
  5. Ao L, Zeng XC, Nie Y, Mu Y, Zhou L et al. Flavobacterium arsenatis sp. nov., a novel arsenic-resistant bacterium from high-arsenic sediment. Int J Syst Evol Microbiol 2014; 64:3369–3374 [View Article][PubMed]
     [Google Scholar]
  6. Ryu SH, Park M, Jeon Y, Lee JR, Park W et al. Flavobacterium filum sp. nov., isolated from a wastewater treatment plant in Korea. Int J Syst Evol Microbiol 2007; 57:2026–2030 [View Article][PubMed]
     [Google Scholar]
  7. Liu H, Liu R, Yang SY, Gao WK, Zhang CX et al. Flavobacterium anhuiense sp. nov., isolated from field soil. Int J Syst Evol Microbiol 2008; 58:756–760 [View Article][PubMed]
     [Google Scholar]
  8. Kämpfer P, Lodders N, Martin K, Avendaño-Herrera R. Flavobacterium chilense sp. nov. and Flavobacterium araucananum sp. nov., isolated from farmed salmonid fish. Int J Syst Evol Microbiol 2012; 62:1402–1408 [View Article][PubMed]
     [Google Scholar]
  9. Kämpfer P, Busse HJ, Mcinroy JA, Xu J, Glaeser SP. Flavobacterium nitrogenifigens sp. nov., isolated from switchgrass (Panicum virgatum). Int J Syst Evol Microbiol 2015; 65:2803–2809 [View Article][PubMed]
     [Google Scholar]
  10. von Bulow JFW, Dobereiner J. Potential for nitrogen fixation in maize genotypes in Brazil. Proc Natl Acad Sci 1975; 72:2389–2393 [View Article]
     [Google Scholar]
  11. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
     [Google Scholar]
  12. Hall TA. BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symp Ser London: Information Retrieval Ltd; 1999 pp. c1979–c2000
     [Google Scholar]
  13. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
     [Google Scholar]
  15. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  16. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 1971; 20:406–416 [View Article]
     [Google Scholar]
  17. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
     [Google Scholar]
  18. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  19. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
     [Google Scholar]
  20. Zhou Y, Wang X, Liu H, Zhang KY, Zhang YQ et al. Pontibacter akesuensis sp. nov., isolated from a desert soil in China. Int J Syst Evol Microbiol 2007; 57:321–325 [View Article][PubMed]
     [Google Scholar]
  21. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark: MIDI, Inc; 1990
     [Google Scholar]
  22. Komagata K, Suzuki K-I. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1988161–207
     [Google Scholar]
  23. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  24. Taibi G, Schiavo MR, Gueli MC, Rindina PC, Muratore R et al. Rapid and simultaneous high-performance liquid chromatography assay of polyamines and monoacetylpolyamines in biological specimens. J Chromatogr B Biomed Sci Appl 2000; 745:431–437 [View Article][PubMed]
     [Google Scholar]
  25. Busse H-J, Bunka S, Hensel A, Lubitz W. Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 1997; 47:698–708 [View Article]
     [Google Scholar]
  26. Horn MA, Ihssen J, Matthies C, Schramm A, Acker G et al. Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa . Int J Syst Evol Microbiol 2005; 55:1255–1265 [View Article][PubMed]
     [Google Scholar]
  27. Suwannachart C, Rueangyotchanthana K, Srichuay S, Pheng S, Fungsin B et al. Flavobacterium tistrianum sp. nov., a gliding bacterium isolated from soil. Int J Syst Evol Microbiol 2016; 66:2241–2246 [View Article][PubMed]
     [Google Scholar]
  28. Son HM, Kook M, Park SY, Mavlonov GT, Yi TH. Flavobacterium kyungheensis sp. nov., isolated from soil of a ginseng field. Antonie van Leeuwenhoek 2013; 104:1029–1037 [View Article][PubMed]
     [Google Scholar]
  29. Yang JE, Kim SY, Im WT, Yi TH. Flavobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside converting activity isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2011; 61:1408–1412 [View Article][PubMed]
     [Google Scholar]
  30. Bu JH, Cha CJ. Flavobacterium foetidum sp. nov., isolated from ginseng soil. Int J Syst Evol Microbiol 2018; 68:616–622 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003717
Loading
Flavobacterium ustbae sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia
Int J Syst Evol Microbiol 69, 3955 (2019); https://doi.org/10.1099/ijsem.0.003717
/content/journal/ijsem/10.1099/ijsem.0.003717
/content/journal/ijsem/10.1099/ijsem.0.003717
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error