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Volume 71, Issue 3

sp. nov., an endophytic actinomycete isolated from the roots of Free

Abstract

A novel endophytic actinomycete, designated strain Gen 01, was isolated from the roots of and characterized by using a polyphasic approach. The predominant cellular fatty acids were iso-C, summed feature 3, iso H-C, C and iso-C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phospholipids of unknown structure containing glucosamine inositol, phosphatidylinositol and unidentified phospholipids. The major menaquinone was MK-8 (H). The DNA G+C content of the genome sequence, consisting of 7 177 725 bp, was 74.5 mol%. Phylogenetic analysis of the full-length 16S rRNA gene sequences showed that strain Gen 01 belongs to the genus with the highest sequence similarity to CGMCC 4.1532 (98.9 %) and lower than 98.7 % similarity to other species of the genus with validly published names. The average nucleotide identity and digital DNA–DNAhybridization values between strain Gen 01 and CGMCC 4.1532 were 84.6 and 30.9 %, respectively. Furthermore, the morphological, physiological and biochemical characteristics were sufficient to categorize strain Gen 01 as being distinct from CGMCC 4.1532. Consequently, based on phenotypic and genotypic characteristics, strain Gen 01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Gen 01 (=CICC 24820=JCM 33840).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Broussonetia papyrifera , polyphasic taxonomy and Pseudonocardia broussonetiae sp. nov
Funding
This study was supported by the:
  • China Postdoctoral Science Foundation (Award 2020M682602)
    • Principle Award Recipient: ZhenggangXu
  • Key Technology R&D Program of Changsha (Award kq1901145)
    • Principle Award Recipient: YunlinZhao
  • Major Science and Technology Program of Hunan Province (Award 2017NK1014)
    • Principle Award Recipient: YunlinZhao
  • Forestry Science and Technology Project of Hunan Province (Award XLK201920)
    • Principle Award Recipient: YunlinZhao
  • State Forestry and Grassland Bureau (Award 2018-01)
    • Principle Award Recipient: YunlinZhao
© 2021 The Authors
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2024-05-18
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Pseudonocardia broussonetiae sp. nov., an endophytic actinomycete isolated from the roots of Broussonetia papyrifera
Int J Syst Evol Microbiol 71, 004680 (2021); https://doi.org/10.1099/ijsem.0.004680
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