- Volume 61, Issue 12, 2011
Volume 61, Issue 12, 2011
- Notification List
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Notification that new names and new combinations have appeared in volume 61, part 9, of the IJSEM
This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed according to the Rules of priority (i.e. page number and order of valid publication of names in the original articles). Taxonomic opinions included in this List (i.e. the creation of synonyms or the emendation of circumscriptions) cannot be considered as validly published nor, in any other way, approved by the International Committee on Systematics of Prokaryotes and its Judicial Commission.
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- New Taxa
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- Actinobacteria
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Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential
More LessA Gram-positive-staining, aerobic, non-motile, coccoid shaped, halotolerant bacterium (strain JG 06T) was isolated from the roots of Salicornia brachiata, an extreme halophyte. Phylogenetic analysis based on 16S rRNA gene sequence showed that the novel strain had sequence similarities of 99.2 % to Brachybacterium paraconglomeratum JCM 11608T, 99.0 % to Brachybacterium conglomeratum DSM 10241 and 98.2 % to Brachybacterium faecium DSM 4810T. DNA–DNA hybridization with B. paraconglomeratum DSM 46341T, B. conglomeratum DSM 10241T, B. faecium DSM 4810T, Brachybacterium tyrofermentans DSM 10673T, Brachybacterium alimentarium DSM 10672T, Brachybacterium fresconsis DSM 14564T, Brachybacterium sacelli DSM 14566T and Brachybacterium muris DSM 15460T resulted in reassociation values of 36.2 %, 36.5 %, 35.8 %, 27.6 %, 27.9 %, 28.2 %, 28.7 % and 11.2 %, respectively. The peptidoglycan type of strain JG 06T was variant A4γ. The menaquinone content was MK7 (100 %). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl diglyceride, three unidentified phospholipids and three glycolipids. The predominant fatty acid was anteiso-C15 : 0 (52.07 %); significant amounts of iso-C16 : 0 (12.38 %), iso-C15 : 0 (8.59 %) and anteiso-C17 : 0 (10.03 %) were also present. The G+C content of the DNA was 73.0 mol%. The strain formed a growth pellicle in nitrogen-free semisolid NFb medium containing NaCl at levels of up to 4 % (w/v) and reduced acetylene to ethylene, a result indicative of N2 fixation. In nutrient broth medium the novel strain grew at NaCl concentrations up to 15 % (w/v). It also had the ability to produce indole-3-acetic acid (IAA) and siderophores, utilized 1-aminocyclopropane-1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. On the basis of physiological, biochemical data and phylogenetic analyses, strain JG 06T should be placed in the genus Brachybacterium. Strain JG 06T represents a novel species of the genus Brachybacterium for which the name Brachybacterium saurashtrense sp. nov. is proposed (type strain JG 06T = DSM 23186T = IMCC 252T).
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Spinactinospora alkalitolerans gen. nov., sp. nov., an actinomycete isolated from marine sediment
More LessA novel marine actinomycete, designated CXB654T, was isolated from marine sediment collected at a depth of 17.5 m near the Yellow Sea Cold Water Mass, China. Optimal growth occurred at 37.0 °C, at pH 7.0–8.0 and in 3–8 % (w/v) NaCl. Strain CXB654T formed branched substrate mycelium without fragmentation. Abundant aerial mycelium differentiated into long or short chains of spores and spores were elliptical and cylindrical with spiny surfaces. Strain CXB654T contained meso-diaminopimelic acid as the diagnostic diamino acid, and ribose and glucose as the major whole-cell components. Phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. MK-10(H8), MK-10(H6) and MK-9(H8) were the predominant menaquinones. The major fatty acids were i-C16 : 0 (24.46 %), ai-C17 : 0 (20.66 %) and C18 : 0 (20.14 %). The DNA G+C content was 71.1 mol%. Comparative analysis of 16S rRNA gene sequences showed that the novel strain was most closely related to genera within the family Nocardiopsaceae, but formed a separate lineage. Highest sequence similarities were to Murinocardiopsis flavida DSM 45312T (96.6 %), Thermobifida halotolerans YIM 90462T (96.5 %) and Marinactinospora thermotolerans DSM 45154T (96.1 %). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain CXB654T was considered to represent a novel species in a new genus in the family Nocardiopsaceae, and the name Spinactinospora alkalitolerans gen. nov., sp. nov. is proposed; the type strain is CXB654T ( = DSM 45414T = LMG 25485T).
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Streptomyces fenghuangensis sp. nov., isolated from seawater
More LessAn actinomycete, designated strain GIMN4.003T, was isolated from seawater collected in Sanya, China. It produced white aerial mycelium and yellow substrate mycelium on Gause’s synthetic agar medium no. 1. The substrate mycelium colour was not sensitive to pH. Scanning electron microscopy observations revealed that GIMN4.003T produced straight to flexuous spore chains of rough to warty spores. ll-Diaminopimelic acid was present in the cell-wall hydrolysate. Based on chemotaxonomy and morphological features, strain GIMN4.003T was identified as a member of the genus Streptomyces. Melanin was not produced. No antimicrobial activity was detected against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Penicillium citrinum or Candida albicans. Analysis of the 16S rRNA gene sequence revealed that the highest sequence similarity was to Streptomyces radiopugnans R97T (99.0 %). However, DNA relatedness between GIMN4.003T and S. radiopugnans DSM 41901T was low (41.24±1.47 %). Furthermore, the morphological, physiological and biochemical characteristics of strain GIMN4.003T were different from those of S. radiopugnans DSM 41901T and the type strains of other closely related Streptomyces species. On the basis of its physiological and molecular properties, it is evident that strain GIMN4.003T ( = CCTCCM 208215T = NRRL B-24801T) represents the type strain of a novel species within the genus Streptomyces, for which the name Streptomyces fenghuangensis sp. nov. is proposed.
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Micrococcus lactis sp. nov., isolated from dairy industry waste
More LessA Gram-positive, yellow-pigmented, actinobacterial strain, DW152T, was isolated from a dairy industry effluent treatment plant. 16S rRNA gene sequence analysis indicated that strain DW152T exhibited low similarity with many species with validly published names belonging to the genera Micrococcus and Arthrobacter. However, phenotypic properties including chemotaxonomic markers affiliated strain DW152T to the genus Micrococcus. Strain DW152T had ai-C15 : 0 and i-C15 : 0 as major cellular fatty acids, and MK-8(H2) as the major menaquinone. The cell-wall peptidoglycan of strain DW152T had l-lysine as the diagnostic amino acid and the type was A4α. The DNA G+C content of strain DW152T was 68.0 mol%. In 16S rRNA gene sequence analysis, strain DW152T exhibited significant similarity with Micrococcus terreus NBRC 104258T, but the mean value of DNA–DNA relatedness between these strains was only 42.3 %. Moreover, strain DW152T differed in biochemical and chemotaxonomic characteristics from M. terreus and other species of the genus Micrococcus. Based on the above differences, we conclude that strain DW152T should be treated as a novel species of the genus Micrococcus, for which the name Micrococcus lactis sp. nov. is proposed. The type strain of Micrococcus lactis sp. nov. is DW152T ( = MTCC10523T = DSM 23694T).
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Sphaerisporangium krabiense sp. nov., isolated from soil
A Gram-staining-positive, filamentous bacterial strain, designated A-T 0308T, was isolated from soil of a tropical mangrove forest in Thailand. Strain A-T 0308T developed spherical sporangia containing non-motile spores on aerial mycelium. The novel strain contained meso-diaminopimelic acid, N-acetyl-type peptidoglycan and madurose, mannose, ribose, galactose and glucose as whole-cell sugars. The predominant menaquinones were MK-9(H4) and MK-9(H6); a small amount of MK-9(H2) and MK-9 was also detected. Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and phosphoglycolipid. The predominant cellular fatty acids were iso-C16 : 0 and 10-methylated C17 : 0. The G+C content of the DNA was 72 mol%. Phenotypic and chemotaxonomic analyses showed that the novel isolate had characteristics typical of members of the genus Sphaerisporangium. 16S rRNA gene sequence analysis also indicated that the strain belongs to the genus Sphaerisporangium and that it represents a clade distinct from other members of the genus with sequence similarities ranging from 96.3 to 97.8 % between the novel strain and its closest relatives. Based on the results of phenotypic, chemotaxonomic and phylogenetic studies, strain A-T 0308T ( = BCC 21702T = NBRC 107571T) represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium krabiense sp. nov. is proposed.
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Descriptions of Actinoplanes ianthinogenes nom. rev. and Actinoplanes octamycinicus corrig. comb. nov., nom. rev.
More LessPhylogenetic analysis of ‘Actinoplanes ianthinogenes’ Coronelli et al. 1974 and ‘Actinoplanes ianthinogenes subsp. octamycini’ Gauze et al. 1979 based on 16S rRNA gene sequencing data revealed that these organisms form a clade in the family Micromonosporaceae. Morphological and chemotaxonomic characteristics of strains of these species were consistent with those of members of the genus Actinoplanes. Morphological, DNA–DNA hybridization, physiological, biochemical and chemotaxonomic data showed that ‘A. ianthinogenes’ and ‘A. ianthinogenes subsp. octamycini’ can be easily differentiated from each other and that they merit separate species status. On the basis of morphological, physiological, biochemical, chemotaxonomic and DNA–DNA hybridization data, it is concluded that ‘A. ianthinogenes’ and ‘A. ianthinogenes subsp. octamycini’ should be assigned the status of two novel species: Actinoplanes ianthinogenes nom. rev. (type strain NBRC 13996T = A/1668T = ATCC 21884T = BCRC 13611T = DSM 43864T = IMSNU 20032T = JCM 3249T = KCTC 9347T = KCTC 9592T = NCIMB 12639T = NRRL B-16720T) and Actinoplanes octamycinicus corrig. comb. nov., nom. rev. (type strain NBRC 14524T = INA 4041T = ATCC 43632T = JCM 9649T = KCTC 9593T), respectively.
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Actinophytocola xinjiangensis sp. nov., isolated from virgin forest soil
More LessAn aerobic, non-motile actinobacterium, strain QAIII60T, was isolated from virgin forest soil of Kanas Nature Reserve, Xinjiang, north-west China. The isolate produced a very scant aerial mycelium that fragmented into cylindrical spores and a non-fragmented substrate mycelium with occasional septa. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose, galactose, glucose, ribose and rhamnose (trace). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. The major cellular fatty acids were iso-C16 : 0, iso-C14 : 0, iso-C16 : 1 H and C17 : 1ω6c. The isoprenoid quinones consisted of MK-9(H4) and MK-10(H2). The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QAIII60T formed a distinct phyletic line that was most closely, albeit loosely, associated with the genus Actinophytocola. A number of physiological characteristics differentiated the isolate from members of the genus Actinophytocola. On the basis of these data, we propose that strain QAIII60T ( = CGMCC 4.4663T = NBRC 106673T) be assigned as the type strain of a novel species, Actinophytocola xinjiangensis sp. nov.
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Nocardia artemisiae sp. nov., an endophytic actinobacterium isolated from a surface-sterilized stem of Artemisia annua L.
More LessA novel actinobacterium, designated YIM 65623T, was isolated from a surface-sterilized stem of Artemisia annua L. Strain YIM 65623T had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus Nocardia. Growth occurred with 0–7 % (w/v) NaCl (optimum 0–3 %), at pH 5.0–9.0 (optimum pH 6.0) and at 10–37 °C (optimum 20–28 °C). Comparative 16S rRNA gene sequence analysis showed that strain YIM 65623T constituted a distinct sublineage within the genus Nocardia and displayed 94.1–98.2 % sequence similarity to members of established species in the genus Nocardia. However, DNA–DNA relatedness and physiological and biochemical characteristics showed that strain YIM 65623T could be differentiated from its closest phylogenetic relatives. The G+C content of the genomic DNA was 69.6 mol%. It is proposed that strain YIM 65623T be classified as a representative of a novel species, Nocardia artemisiae sp. nov. The type strain is YIM 65623T ( = DSM 45379T = CCTCC AA 209038T).
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Phytomonospora endophytica gen. nov., sp. nov., isolated from the roots of Artemisia annua L.
More LessA novel endophytic actinomycete, strain YIM 65646T, was isolated from the roots of Artemisia annua L. collected from Yunnan Province, south-west China. Growth was observed in the temperature range 10–40 °C (optimum 20–28 °C) and at pH 6.0–9.0 (optimum pH 7.0). The organism formed well-developed, branched substrate mycelia, but aerial mycelium was not produced. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain YIM 65646T belonged to the family Micromonosporaceae (sharing ≤93.6 % sequence similarity with members of this family) and formed a distinct clade in the Micromonosporaceae phylogenetic tree. The strain contained meso-diaminopimelic acid in the cell wall and mannose, ribose, galactose and glucose in whole-cell hydrolysates. The major menaquinones were MK-10(H4), MK-10(H2), MK-8(H2), MK-9(H2) and MK-10(H6). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, iso-C17 : 0 and iso-C16 : 0. The DNA G+C content of strain YIM 65646T was 70.0 mol%. On the basis of morphological and chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain should be classified in a novel genus and species, Phytomonospora endophytica gen. nov., sp. nov., in the family Micromonosporaceae. The type strain is YIM 65646T ( = CCTCC AA 209041T = DSM 45386T).
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Frondihabitans cladoniiphilus sp. nov., an actinobacterium of the family Microbacteriaceae isolated from lichen, and emended description of the genus Frondihabitans
More LessA novel actinobacterium, designated strain CafT13T, was isolated from the thallus of the reindeer lichen Cladonia arbuscula sampled in the Austrian Alps (Koralpe). The organism was aerobic, with rod- to irregular-shaped cells (often forming dense clusters of cells when grown in liquid medium), Gram-stain-positive, oxidase-negative, catalase-positive and non-motile. It was able to grow at 1 °C and at low to neutral pH, but not above 30 °C or at high pH. The peptidoglycan type was B2β with ornithine as the diagnostic diamino acid. The menaquinones were MK-7 and MK-8. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids and one unidentified aminolipid. The predominant fatty acids were C18 : 1, C14 : 0 2-OH, C17 : 1ω9c, C16 : 0 and anteiso-C15 : 0. The mean DNA G+C content of strain CafT13T was 69.0±0.17 mol%. 16S rRNA gene sequence analysis showed that strain CafT13T belongs to the family Microbacteriaceae, within the genus Frondihabitans. The mean level of DNA–DNA relatedness between strain CafT13T and the type strain of Frondihabitans australicus was 35.2±5.23 %. The enzyme spectrum of strain CafT13T differentiated it from recognized species of the genus Frondihabitans. Based on molecular, chemotaxonomic and physiological data, strain CafT13T is considered to represent a novel species of the genus Frondihabitans, for which the name Frondihabitans cladoniiphilus sp. nov. is proposed; the type strain is CafT13T ( = DSM 23273T = LMG 25550T).
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- Proteobacteria
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Proposal that the arsenite-oxidizing organisms Thiomonas cuprina and ‘Thiomonas arsenivorans’ be reclassified as strains of Thiomonas delicata, and emended description of Thiomonas delicata
The three As(III)-oxidizing members of the class Betaproteobacteria Thiomonas delicata, Thiomonas cuprina and ‘Thiomonas arsenivorans’ were isolated from mining sites in geographically distinct areas, namely Japan, Germany and France, respectively. They are all able to oxidize As(III) but only ‘T. arsenivorans’ and T. cuprina show efficient autotrophic growth with As(III) and are able to grow on a sole carbon source. These two organisms are also motile, whereas T. delicata is not. Only T. cuprina can grow autotrophically on chalcopyrite. The three strains share >99 % gene sequence similarity with each other based on their 16S rRNA genes and 16S–23S ITS regions. DNA–DNA hybridization results are above, or close to, the threshold value of 70 % recommended for the definition of bacterial species. The three taxa show very similar fatty acid profiles with differences only in five minor fatty acid components. They possess phylogenetic and chemotaxonomic similarities supporting the reclassification of these taxa as a single species. We propose that ‘T. arsenivorans’ and T. cuprina be reassigned as strains of T. delicata (type strain DSM 17897T).
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Acinetobacter rudis sp. nov., isolated from raw milk and raw wastewater
Two bacterial strains, G30T and A1PC16, isolated respectively from raw milk and raw wastewater, were characterized using a polyphasic approach. Chemotaxonomic characterization supported the inclusion of these strains in the genus Acinetobacter, with Q-8 and Q-9 as the major respiratory quinones, genomic DNA G+C contents within the range observed for this genus (38–47 mol%) and C16 : 0, C18 : 1ω9c and C16 : 1ω7c/iso-C15 : 0 2-OH as the predominant fatty acids. The observation of 16S rRNA gene sequence similarity lower than 97 % with other Acinetobacter species with validly published names led to the hypothesis that these isolates could represent a novel species. This hypothesis was supported by comparative analysis of partial sequences of the genes rpoB and gyrB, which showed that strains G30T and A1PC16 did not cluster with any species with validly published names, forming a distinct lineage. DNA–DNA hybridizations confirmed that the two strains were members of the same species, which could be distinguished from their congeners by several phenotypic characteristics. On the basis of these arguments, it is proposed that strains G30T and A1PC16 represent a novel species, for which the name Acinetobacter rudis sp. nov. is proposed. The type strain is strain G30T ( = LMG 26107T = CCUG 57889T = DSM 24031T = CECT 7818T).
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Olivibacter oleidegradans sp. nov., a hydrocarbon-degrading bacterium isolated from a biofilter clean-up facility on a hydrocarbon-contaminated site
A novel hydrocarbon-degrading, Gram-negative, obligately aerobic, non-motile, non-sporulating, rod-shaped bacterium, designated strain TBF2/20.2T, was isolated from a biofilter clean-up facility set up on a hydrocarbon-contaminated site in Hungary. It was characterized by using a polyphasic approach to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate is affiliated with the genus Olivibacter in the family Sphingobacteriaceae. It was found to be related most closely to Olivibacter ginsengisoli Gsoil 060T (93.3 % 16S rRNA gene sequence similarity). Strain TBF2/20.2T grew at pH 6–9 (optimally at pH 6.5–7.0) and at 15–42 °C (optimally at 30–37 °C). The major fatty acids were iso-C15 : 0 (39.4 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c; 26.0 %), iso-C17 : 0 3-OH (14.5 %) and C16 : 0 (4.5 %). The major menaquinone was MK-7 and the predominant polar lipid was phosphatidylethanolamine. The DNA G+C content of strain TBF2/20.2T was 41.2 mol%. Physiological and chemotaxonomic data further confirmed the distinctiveness of strain TBF2/20.2T from recognized members of the genus Olivibacter. Thus, strain TBF2/20.2T is considered to represent a novel species of the genus Olivibacter, for which the name Olivibacter oleidegradans sp. nov. is proposed. The type strain is TBF2/20.2T ( = NCAIM B 02393T = CCM 7765T).
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Catenovulum agarivorans gen. nov., sp. nov., a peritrichously flagellated, chain-forming, agar-hydrolysing gammaproteobacterium from seawater
More LessA novel Gram-negative, strictly aerobic, agar-hydrolysing bacterium, designated YM01T, was isolated from seawater samples collected from the Yellow Sea (coastal region of Qingdao, PR China). Cells were rod-shaped, peritrichously flagellated and formed long chains end-to-end. The isolate had an absolute requirement for Na+ ions, but not seawater, for growth and grew optimally at about 28 °C, in 2 % NaCl and at pH 8.0–9.0. The isolate could not be cultured in marine broth 2216, but grew well on marine agar 2216. YM01T was able to hydrolyse cellulose, starch, aesculin and Tween 80, but not egg yolk, gelatin, urea or casein. 16S rRNA gene sequence analysis demonstrated that this isolate was unique, showing only 88.4–91.0 % sequence similarity to its closest neighbours, including members of the genera Glaciecola (88.4–91.0 %), Alteromonas (88.7–89.6 %), Aestuariibacter (89.3–90.4 %), Salinimonas (89.0 %), Bowmanella (90.1–90.3 %) and Agarivorans (88.5–89.9 %). Phylogenetic analyses demonstrated that strain YM01T formed a distinct clade closely related to species of the family Alteromonadaceae within the group of Alteromonas-like gammaproteobacteria. It contained menaquinone MK-7 as the predominant isoprenoid quinone and C16 : 0 (38.3 %), C16 : 1ω7c and/or iso-C15 : 0 2-OH (29.0 %), C18 : 1ω7c (9.3 %) and C10 : 0 3-OH (8.2 %) as major cellular fatty acids. Phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid were the major phospholipid constituents. The DNA G+C content was 44.8 mol%. Based on its phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain YM01T is considered to represent a novel species in a new genus in the Gammaproteobacteria, for which the name Catenovulum agarivorans gen. nov., sp. nov. is proposed; the type strain of Catenovulum agarivorans is YM01T ( = CGMCC 1.10245T = DSM 23111T = JCM 16580T).
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Oceanisphaera ostreae sp. nov., isolated from seawater of an oyster farm, and emended description of the genus Oceanisphaera Romanenko et al. 2003
More LessA Gram-stain-negative, motile, non-spore-forming and short rod- or rod-shaped bacterial strain, T-w6T, was isolated from seawater of an oyster farm in the South Sea, Korea. Strain T-w6T grew optimally at 25 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain T-w6T joined the cluster comprising Oceanisphaera species with a bootstrap resampling value of 90.8 %, and this cluster joined the clade comprising members of the genera Oceanimonas and Zobellella with a bootstrap resampling value of 100 %. Strain T-w6T exhibited 16S rRNA gene sequence similarity of 95.9 and 96.6 % to the type strains of Oceanisphaera litoralis and Oceanisphaera donghaensis, respectively. Strain T-w6T and the type strains of Oceanisphaera litoralis and Oceanisphaera donghaensis had Q-8 as the predominant ubiquinone and iso-C15 : 0 2-OH and/or C16 : 1ω7c, C18 : 1ω7c and C16 : 0 as the major fatty acids. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain T-w6T was 56.6 mol%. Mean DNA–DNA relatedness of strain T-w6T with Oceanisphaera litoralis DSM 15406T and Oceanisphaera donghaensis KCTC 12522T was 13 and 10 %, respectively. Phenotypic properties of strain T-w6T demonstrated that this strain could be distinguished from the other Oceanisphaera species. On the basis of the data presented, strain T-w6T is considered to represent a novel species of the genus Oceanisphaera, for which the name Oceanisphaera ostreae sp. nov. is proposed; the type strain is T-w6T ( = KCTC 23422T = CCUG 60525T). An emended description of the genus Oceanisphaera is also presented.
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Description of Spongiibacter borealis sp. nov., isolated from Arctic seawater, and reclassification of Melitea salexigens Urios et al. 2008 as a later heterotypic synonym of Spongiibacter marinus Graeber et al. 2008 with emended descriptions of the genus Spongiibacter and Spongiibacter marinus
More LessA Gram-negative, rod-shaped and motile strain, designated CL-AS9T, was isolated from polar seawater of the Arctic. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Spongiibacter, sharing 93.9 % and 93.7 % sequence similarities with the type strains of Spongiibacter tropicus CL-CB221T and Spongiibacter marinus HAL40bT, respectively. Phylogenetic analyses revealed that strain CL-AS9T formed a separate branch that was distinct from a clade comprising Spongiibacter marinus HAL40bT, Spongiibacter tropicus CL-CB221T and Melitea salexigens 5IX/A01/131T. Cells of the strain grew optimally at 20–25 °C and pH 6.6–8.0 in the presence of 3–4 % (w/v) sea salts. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified aminophospholipid. The major quinone was ubiquinone 8. The major cellular fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH (23.1 %), C17 : 1ω8c (22.1 %) and C18 : 1ω7c (15.6 %). The genomic DNA G+C content was 53.6 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, we propose the name Spongiibacter borealis sp. nov. with the type strain CL-AS9T ( = KCCM 90094T = JCM 17304T) and the reclassification of Melitea salexigens as a later heterotypic synonym of Spongiibacter marinus. We also provide emended descriptions of the genus Spongiibacter and Spongiibacter marinus.
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Pusillimonas harenae sp. nov., isolated from a sandy beach, and emended description of the genus Pusillimonas
A Gram-stain-negative, motile bacterium with two lateral flagella, designated strain B201T, was isolated from beach sand from the Taean coast in South Korea. Cells were ovoid rods and positive for catalase and oxidase. Growth of strain B201T was observed between 15 and 45 °C (optimum, 30 °C) and between pH 5.0 and 9.0 (optimum, pH 6.0–7.5). Strain B201T contained ubiquinone Q-8 as the major isoprenoid quinone, but MK-6 was also present as a minor quinone. The major fatty acids of strain B201T were C17 : 0 cyclo, C16 : 0, summed feature 2 (iso-C16 : 1 I/C14 : 0 3-OH and/or C12 : 0 ALDE), C12 : 0 and C19 : 0 cyclo ω8c. The major cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and three aminolipids. The genomic DNA G+C content was 53.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a phyletic lineage with Pusillimonas ginsengisoli DCY25T within the genus Pusillimonas. Strain B201T was most closely related to P. ginsengisoli DCY25T and Pusillimonas soli MJ07T with similarities of 98.6 and 97.5 %, respectively. However, DNA–DNA relatedness values of strain B201T with P. ginsengisoli DCY25T and P. soli MJ07T were 30.2±5.4 and 4.9±1.8 %, respectively. On the basis of chemotaxonomic data and molecular properties, strain B201T represents a novel species of the genus Pusillimonas, for which the name Pusillimonas harenae sp. nov. is proposed; the type strain is B201T ( = KACC 14927T = JCM 16917T). An emended description of the genus Pusillimonas is given.
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Bradyrhizobium cytisi sp. nov., isolated from effective nodules of Cytisus villosus
More LessSeveral strains isolated from Cytisus villosus nodules have been characterized based on their diverse genetic, phenotypic and symbiotic characteristics. According to 16S rRNA gene sequence analysis, the isolates formed a group that was closely related to Bradyrhizobium canariense BTA-1T with 99.4 % similarity. Analysis of three housekeeping genes, recA, atpD and glnII, suggested that the C. villosus strains represent a novel Bradyrhizobium species most closely related to B. canariense BTA-1T with similarities of 94.2, 96.7 and 94.5 %, respectively. All these differences were congruent with DNA–DNA hybridization analysis, which revealed 31 % relatedness between a representative strain (CTAW11T) isolated from C. villosus nodules and B. canariense BTA-1T. Phenotypic differences among the strains isolated from C. villosus and B. canariense were based on assimilation of carbon and nitrogen sources. The nodC and nifH genes of strain CTAW11T were phylogenetically related to those of strains belonging to bv. genistearum and divergent from those of bv. glycinearum and, accordingly, they do not nodulate soybean. Based on the genotypic and phenotypic data obtained in this study, our strains should be classified as representatives of a novel species for which the name Bradyrhizobium cytisi sp. nov. is proposed; the type strain is CTAW11T ( = LMG 25866T = CECT 7749T).
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Altererythrobacter ishigakiensis sp. nov., an astaxanthin-producing bacterium isolated from a marine sediment
A Gram-negative, non-motile, non-spore-forming, halophilic rod, designated JPCCMB0017T, was isolated from a marine sediment of the coastal area of Okinawa, Japan. The isolate formed orange–red colonies on marine agar. Bacteriochlorophyll α was absent and sphingoglycolipid 1 and other carotenoids, including astaxanthin, adonixanthin and zeaxanthin, were present. Ubiquinone-10 (Q-10) was the main respiratory quinone and C18 : 1ω7c was the major cellular fatty acid. The G+C content of DNA was 59.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus Altererythrobacter in the family Erythrobacteraceae. Strain JPCCMB0017T exhibited 96.8 % 16S rRNA gene sequence similarity with Altererythrobacter marinus H32T. Unlike other members of the genus Altererythrobacter, strain JPCCMB0017T reduced nitrate. On the basis of genotypic and phenotypic data, a novel species is proposed to accommodate this isolate, with the name Altererythrobacter ishigakiensis sp. nov. The type strain is JPCCMB0017T ( = NITE-AP48T = ATCC BAA-2084T = NBRC 107699T).
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