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f sp. nov., an actinobacterium isolated from a soil sample

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  • Authors: Jian-Rong Huang1,† ,  Hong Ming2,3,† ,  Shuai Li1 ,  Xiao-Lin Meng1 ,  Jian-Xin Zhang1 ,  Thi-Nhan Khieu3,4 ,  Zhong Tang5 ,  Wen-Jun Li3,6 , Guo-Xing Nie1
    • VIEW AFFILIATIONS
    • 1 1College of Fisheries, Henan Normal University,Xinxiang, 453007, PRChina 2 2College of Life Sciences and Technology, Xinxiang Medical University,Xinxiang, 453003, PRChina 3 3Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091, PRChina 4 4Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology,Hanoi,Vietnam 5 5College of information and Management, Guangxi Medical University,Nanning, 530021, PRChina 6 6State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275, PRChina
  • Wen-Jun Li [email protected]
  • First Published Online: 01 May 2016, International Journal of Systematic and Evolutionary Microbiology 66: 2002-2007, doi: 10.1099/ijsem.0.000978
  • Subject: NEW TAXA - Actinobacteria
  • Cover date:
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  • A novel Gram-reaction-positive, non-motile, aerobic bacterium, designated CFH S0483, was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. 16S rRNA gene sequence analysis showed that the strain is a member of the genus and has highest 16S rRNA gene sequence similarities with DSM 16389 (97.3 %) and DSM 43045 (97.1 %), and similarities  < 97.0 % with type strains of other species of the genus . Strain CFH S0483 was able to grow at 10–37 °C, at pH 7.0–9.0 and tolerated NaCl up to 2.0 % (w/v). The whole-cell sugars were mannose, galactose, glucose and ribose. The isolate contained -2,4-diaminobutyric acid, -alanine, -glutamic acid and glycine in the cell-wall peptidoglycan. Strain CFH S0483 exhibited a menaquinone system with MK-12, and the major fatty acids were anteiso-C, anteiso-C and iso-C. The genomic DNA G+C content of strain CFH S0483 was 71.6 mol%. Based on the phylogenetic and phenotypic analysis, and low DNA–DNA hybridization values, strain CFH S0483 could not be classified into any recognized species of the genus . Strain CFH S0483 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CFH S0483 ( = KCTC 39117 = CCTCC AB 2014301).

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CFH S0483 is KP232915.

  • Four supplementary figures and a supplementary table are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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