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- Volume 66, Issue 5
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f Agromyces insulae sp. nov., an actinobacterium isolated from a soil sample
- Authors: Jian-Rong Huang1,† , Hong Ming2,3,† , Shuai Li1 , Xiao-Lin Meng1 , Jian-Xin Zhang1 , Thi-Nhan Khieu3,4 , Zhong Tang5 , Wen-Jun Li3,6 , Guo-Xing Nie1
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- VIEW AFFILIATIONS
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1 1College of Fisheries, Henan Normal University,Xinxiang, 453007, PRChina 2 2College of Life Sciences and Technology, Xinxiang Medical University,Xinxiang, 453003, PRChina 3 3Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091, PRChina 4 4Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology,Hanoi,Vietnam 5 5College of information and Management, Guangxi Medical University,Nanning, 530021, PRChina 6 6State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275, PRChina
- Correspondence Wen-Jun Li [email protected]
- First Published Online: 01 May 2016, International Journal of Systematic and Evolutionary Microbiology 66: 2002-2007, doi: 10.1099/ijsem.0.000978
- Subject: NEW TAXA - Actinobacteria
- Cover date:




Agromyces insulae sp. nov., an actinobacterium isolated from a soil sample, Page 1 of 1
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A novel Gram-reaction-positive, non-motile, aerobic bacterium, designated CFH S0483T, was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. 16S rRNA gene sequence analysis showed that the strain is a member of the genus Agromyces and has highest 16S rRNA gene sequence similarities with Agromyces humatus DSM 16389T (97.3 %) and Agromyces ramosus DSM 43045T (97.1 %), and similarities < 97.0 % with type strains of other species of the genus Agromyces. Strain CFH S0483T was able to grow at 10–37 °C, at pH 7.0–9.0 and tolerated NaCl up to 2.0 % (w/v). The whole-cell sugars were mannose, galactose, glucose and ribose. The isolate contained l-2,4-diaminobutyric acid, d-alanine, d-glutamic acid and glycine in the cell-wall peptidoglycan. Strain CFH S0483T exhibited a menaquinone system with MK-12, and the major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The genomic DNA G+C content of strain CFH S0483T was 71.6 mol%. Based on the phylogenetic and phenotypic analysis, and low DNA–DNA hybridization values, strain CFH S0483T could not be classified into any recognized species of the genus Agromyces. Strain CFH S0483T is therefore considered to represent a novel species of the genus Agromyces, for which the name Agromyces insulae sp. nov. is proposed. The type strain is CFH S0483T ( = KCTC 39117T = CCTCC AB 2014301T).
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†
These authors contributed equally to this work.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CFH S0483T is KP232915.
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Four supplementary figures and a supplementary table are available with the online Supplementary Material.
© 2016 IUMS | Published by the Microbiology Society
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