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f Propionibacterium namnetense sp. nov., isolated from a human bone infection

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  • Authors: Guillaume Ghislain Aubin1​,2​ ,  Pascale Bémer1​ ,  Stanimir Kambarev3​ ,  Nisha B. Patel4​ ,  Olivier Lemenand5​ ,  Jocelyne Caillon1​,2​ ,  Paul A. Lawson4​,† , Stéphane Corvec1​,6​,†
    • VIEW AFFILIATIONS
    • 1​ 1​Bacteriology and Hygiene department, Nantes University Hospital, 9 quai Moncousu, 44093 Nantes, France 2​ 2​EA3826, Laboratory of clinical and experimental therapeutics of infections, Nantes Medicine School, 1 rue Gaston Veil, 44000 Nantes, France 3​ 3​Institut de Recherche en Santé de l'Université de Nantes INSERM U892 – CNRS 6299 CRCNA Team 13: Nuclear Oncology Research Group: Engineering of Recognition, Nantes, France 4​ 4​Department of Microbiology and Plant Biology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA 5​ 5​Microbiology Laboratory, Saint-Nazaire Hospital, Saint-Nazaire, France 6​ 6​Institut de Recherche en Santé de l'Université de Nantes INSERM U892 – CNRS 6299 CRCNA – Centre de Cancérologie et d'Immunologie Nantes Angers Université de Nantes Nantes France Team 2: Clinical and translational research in skin cancer, Nantes, France
  • Correspondence Stéphane Corvec [email protected]
  • First Published Online: 01 September 2016, International Journal of Systematic and Evolutionary Microbiology 66: 3393-3399, doi: 10.1099/ijsem.0.001204
  • Subject: New taxa - Actinobacteria
  • Received:
  • Accepted:
  • Cover date:
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  • A polyphasic taxonomic study was performed on two Gram-positive-staining, anaerobic, pleomorphic, rod-shaped strains isolated from human bone and tissue samples. Sequencing of the 16S rRNA genes revealed that the strains belong to a novel species within the genus Propionibacterium , most closely related to Propionibacterium acnes subsp. acnes and Propionibacterium acnes subsp. elongatum with similarity values of 98.4 % and 98.1 %, respectively. In addition, protein-coding genes for rpoB, recA and gyrB clearly separated the novel organism from all species and subspecies of the genus Propionibacterium . However, a DNA–DNA hybridization analysis between the novel organism and the type strain P. acnes ATCC 6919 revealed a value of only 61.1 %. Furthermore, whole genome analysis using the program OrthoANI gave a value of 88.5 %, which is significantly below the cut-off value of 95 % for species delineation. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0. The DNA G+C content of the type strain was 59.7 mol%. When taken collectively, phenotypic, molecular genetic, chemotaxonomic and phylogenetic information demonstrate that the organism represents a distinct, albeit close relative of P. acnes On the basis of the results presented, the organism represents a novel member of the genus Propionibacterium for which the name Propionibacterium namnetense sp. nov. is proposed. The type strain is NTS 31307302 (=DSM 29427=CCUG 66358).

  • †

    These authors contributed equally to this work

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA, rpoB, recA and gyrB gene sequences of strain NTS31307302 are KM507346, KM507347, KU847778 and KU847779, respectively.

  • Six supplementary figures and four supplementary tables are available with the online Supplementary Material.

  • Abbreviation:
    MALDI-TOF matrix-assisted laser desorption/ionization time-of-flight

© 2016 IUMS | Published by the Microbiology Society

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