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- Volume 67, Issue 5
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f Glutamicibacter halophytocola sp. nov., an endophytic actinomycete isolated from the roots of a coastal halophyte, Limonium sinense
- Authors: Wei-Wei Feng1,† , Tian-Tian Wang1,† , Juan-Luan Bai1 , Peng Ding1 , Ke Xing1 , Ji-Hong Jiang1 , Xue Peng1 , Sheng Qin1
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- VIEW AFFILIATIONS
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1 The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China
- *Correspondence: Sheng Qin, [email protected]
- First Published Online: 18 May 2017, International Journal of Systematic and Evolutionary Microbiology 67: 1120-1125, doi: 10.1099/ijsem.0.001775
- Subject: New Taxa - Actinobacteria
- Received:
- Accepted:
- Cover date:




Glutamicibacter halophytocola sp. nov., an endophytic actinomycete isolated from the roots of a coastal halophyte, Limonium sinense, Page 1 of 1
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A novel actinobacterium, designated KLBMP 5180T, was isolated from the surface-sterilized root of a coastal halophyte, Limonium sinense, collected from the city of Lianyungang, Jiangsu Province, eastern China. The isolate was Gram-stain-positive, aerobic and non-motile. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine. The predominant menaquinone was MK-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phospholipid, one unidentified glycolipid and two unidentified lipids. anteiso-C15 : 0 and iso-C16 : 0 were the major cellular fatty acids. The DNA G+C content of strain KLBMP 5180T was 60.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KLBMP 5180T belongs to the genus Glutamicibacter and was related most closely to Glutamicibacter nicotianae DSM 20123T (99.3 % similarity), Glutamicibacterarilaitensis Re117T (99.3 %) and Glutamicibacter mysorens LMG 16219T (99.1 %); similarity to other type strains of the genus Glutamicibacter was lower than 98.5 %. However, DNA–DNA relatedness values between strain KLBMP 5180T, G . nicotianae DSM 20123T, G. arilaitensis Re117T and G. mysorens LMG 16219T were 47.5±2.6, 51.3±3.1 and 41.2±4.3 %, respectively. The combination of DNA–DNA hybridization, phylogenetic, phenotypic and chemotaxonomic data supported the suggestion that strain KLBMP 5180T represents a novel species of the genus Glutamicibacter, for which the name Glutamicibacter halophytocola sp. nov. is proposed. The type strain is KLBMP 5180T (=DSM 101718T=KCTC 39692T).
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†
These authors contributed equally to this work.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KLBMP 5180T is JX993762.
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Three supplementary figures are available with the online Supplementary Material.
- Keyword(s): endophytic actinomycete, halophyte, Limonium sinense
© 2017 IUMS | Published by the Microbiology Society
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