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f Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils

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  • Authors: Pablo Martina1​,‡​,† ,  Mariana Leguizamon1​,† ,  Claudia I. Prieto1​ ,  Silvia A. Sousa2​ ,  Patricia Montanaro3​ ,  Walter O. Draghi4​,5​ ,  Maren Stämmler6​ ,  Marisa Bettiol7​ ,  Carla C. C. R. de Carvalho2​ ,  Juliana Palau7​ ,  Cecilia Figoli1​ ,  Florencia Alvarez5​ ,  Silvina Benetti8​ ,  Sergio Lejona8​ ,  Cecilia Vescina7​ ,  Julián Ferreras9​ ,  Peter Lasch6​ ,  Antonio Lagares5​ ,  Angeles Zorreguieta4​ ,  Jorge H. Leitão2​ ,  Osvaldo M. Yantorno1​ , Alejandra Bosch1​
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    • 1​ 1​CINDEFI, CONICET-CCT La Plata, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina 2​ 2​iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal 3​ 3​Hospital Santísima Trinidad de Córdoba, Córdoba, Argentina 4​ 4​Fundación Instituto Leloir and IIBA-Consejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos aires, Argentina 5​ 5​IBBM, CONICET-CCT La Plata, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina 6​ 6​Proteomics and Spectroscopy Unit (ZBS6) at the Centre for Biological Threats and Special Pathogens, Robert Koch-Institut, Berlin, Germany 7​ 7​Sala de Microbiología, Hospital de Niños «Sor María Ludovica», La Plata, Buenos Aires, Argentina 8​ 8​Laboratorio Cemar D. S. L. A. C, Municipalidad de Rosario, Santa Fe, Argentina 9​ 9​IBS, CONICET-CCT Nordeste, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, Argentina ‡​ ‡​Present address: IBS, CONICET-CCT Nordeste, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.
  • *Correspondence: Alejandra Bosch [email protected]
  • First Published Online: 02 November 2017, International Journal of Systematic and Evolutionary Microbiology 68: 14-20, doi: 10.1099/ijsem.0.002293
  • Subject: New Taxa - Proteobacteria
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Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils, Page 1 of 1

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  • Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3 % from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), these isolates clustered with the agricultural soil strain, Burkholderia sp. PBP 78, which was already deposited in the PubMLST database. The isolates were examined using a polyphasic approach, which included 16S rRNA, recA, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities (ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the seven isolates represent a single novel species within the Bcc, for which the name Burkholderia puraquae sp. nov. is proposed. Burkholderia puraquae sp. nov. CAMPA 1040 (=LMG 29660=DSM 103137) was designated the type strain of the novel species, which can be differentiated from other species of the Bcc mainly from recA gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 °C, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities.

  • †

    These authors contributed equally to this work.

  • The GenBank accession numbers for the 16S rRNA, hisA and recA gene sequences of Burkholderia puraquae sp. nov. CAMPA 1043 are: KX516808, KX516814, KX516844, respectively. Those for the 16S rRNA, hisA and recA gene sequences of Burkholderia puraquae sp. nov. CAMPA 1040 (=LMG 29660, DSM 103137) are: KX278717, KX516813, KX516843, respectively. The GenBank accession numbers for the 16S rRNA, hisA and recA gene sequences of Burkholderia puraquae sp. nov. CAMPA 707 are: KX516807, KX516812, KX516842, respectively. The GenBank accession numbers for the 16S rRNA, hisA and recA gene sequences of Burkholderia puraquae sp. nov. CAMPA 567 are: KX516806, KX516811, KX516841, respectively. Those for the 16S rRNA, hisA and recA gene sequences of Burkholderia puraquae sp. nov. CAMPA 566 are: KX516805, KX516810, KX516840 respectively. The GenBank accession numbers for the 16S rRNA, hisA and recA gene sequences of Burkholderia puraquae sp. nov. CAMPA 565 are: KX516804, KX516809, KX516839, respectively. The GenBank accession numbers for the 16S rRNA and recA gene sequences of Burkholderia puraquae sp. nov. PBP78 are: KX534057, KX534063, respectively. The draft genome sequences of Burkholderia sp. CAMPA 1040 was deposited in DDBJ/EMBL/GenBank under the accession numbers NBYX00000000.

  • One supplementary table and four supplementary figures are available with the online version of this article.

© 2018 IUMS | Published by the Microbiology Society

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