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- Volume 68, Issue 3
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oa Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread
- Authors: Cong Wang1,2,3,† , Yan Huang1,3,† , Li Li2 , Jun Guo4 , Zhengyun Wu2 , Yu Deng1,3 , Lirong Dai1,3 , Shichun Ma1,3
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1 1Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China 2 2College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China 3 3Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Chengdu 610041, PR China 4 4Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China
- *Correspondence: Shichun Ma [email protected]
- First Published Online: 10 January 2018, International Journal of Systematic and Evolutionary Microbiology 68: 703-708, doi: 10.1099/ijsem.0.002538
- Subject: New Taxa - Firmicutes and related organisms
- Received:
- Accepted:
- Cover date:
- This is an open access article published by the Microbiology Society under the Creative Commons Attribution License




Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread, Page 1 of 1
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A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus , designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China. The strain was regular rod-shaped. Optimal growth occurred at 37 °C, pH 6.5 with 5.0 g l−1 NaCl. The predominant fatty acids were C18 : 1ω9c, C16 : 0 and C19 : 0 iso. Respiratory quinones were not detected. Seven glycolipids, three lipids, phosphatidylglycerol and diphosphatidylglycerol were detected. The peptidoglycan type A4α l-Lys-d-Asp was determined. Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively. A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus . The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95–96 % threshold value for delineation of genomic prokaryotic species. The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus , for which we propose the name Lactobacillus panisapium sp. nov. The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T).
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†
These authors contributed equally to this work.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and pheS gene sequences of strain Bb 2-3T are KX447147 and KY622000, respectively. The NCBI accession numbers for the draft genome sequences of strain Bb 2-3T, Lactobacillus bombicola DSM 28793T and Lactobacillus apis LMG 26964T are NPNH00000000, NPNG00000000 and PDKP00000000, respectively.
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One supplementary table and six supplementary figures are available with the online version of this article.
- Keyword(s): Lactobacillus, Apis cerana, Lactobacillus panisapium, bee bread
© 2018 IUMS | Published by the Microbiology Society
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1. Kwong WK, Moran NA. Gut microbial communities of social bees. Nat Rev Microbiol 2016; 14: 374– 384 [CrossRef] [PubMed]
-
2. Alberoni D, Gaggìa F, Baffoni L, di Gioia D. Beneficial microorganisms for honey bees: problems and progresses. Appl Microbiol Biotechnol 2016; 100: 9469– 9482 [CrossRef] [PubMed]
-
3. Vásquez A, Forsgren E, Fries I, Paxton RJ, Flaberg E et al. Symbionts as major modulators of insect health: lactic acid bacteria and honeybees. PLoS One 2012; 7: e33188 [CrossRef] [PubMed]
-
4. Rangberg A, Mathiesen G, Amdam GV, Diep DB. The paratransgenic potential of Lactobacillus kunkeei in the honey bee Apis mellifera. Benef Microbes 2015; 6: 513– 523 [CrossRef] [PubMed]
-
5. Olofsson TC, Alsterfjord M, Nilson B, Butler E, Vásquez A. Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera. Int J Syst Evol Microbiol 2014; 64: 3109– 3119 [CrossRef] [PubMed]
-
6. Killer J, Votavová A, Valterová I, Vlková E, Rada V et al. Lactobacillus bombi sp. nov., from the digestive tract of laboratory-reared bumblebee queens (Bombus terrestris). Int J Syst Evol Microbiol 2014; 64: 2611– 2617 [CrossRef] [PubMed]
-
7. Praet J, Meeus I, Cnockaert M, Houf K, Smagghe G et al. Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov. Antonie van Leeuwenhoek 2015; 107: 1337– 1349 [CrossRef] [PubMed]
-
8. Killer J, Dubná S, Sedláček I, Švec P. Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood. Int J Syst Evol Microbiol 2014; 64: 152– 157 [CrossRef] [PubMed]
-
9. Hoang VA, Kim YJ, Nguyen NL, Kim SK, Yang DC. Lactobacillus vespulae sp. nov., isolated from gut of a queen wasp (Vespula vulgaris). Int J Syst Evol Microbiol 2015; 65: 3326– 3332 [CrossRef] [PubMed]
-
10. Wang Cong HY, Ma Shichun DY, Jun GUO, Zhengyun W. Optimization of the fermentation conditions of bee pollen with Lactobacillus sp. strain 2-3. Science and Technology of Food Industry 2016; 37: 119– 123
-
11. Vásquez A, Olofsson TC. The lactic acid bacteria involved in the production of bee pollen and bee bread. J Apic Res 2009; 48: 189– 195 [CrossRef]
-
12. Hungate RE. Chapter IV A roll tube method for cultivation of strict anaerobes. In Norris JR, Ribbons DW. (editors) Methods in Microbiology Millbrae, CA, USA: Academic Press; 1969; pp. 117– 132
-
13. Hungate RE, Macy J. The roll-tube method for cultivation of strict anaerobes. Bulletins from the Ecological Research Committee 1973; 3: 123– 126
-
14. Huang Y, Sun Y, Ma S, Chen L, Zhang H et al. Isolation and characterization of Keratinibaculum paraultunense gen. nov., sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity. FEMS Microbiol Lett 2013; 345: 56– 63 [CrossRef] [PubMed]
-
15. de Bruyne K, Schillinger U, Caroline L, Boehringer B, Cleenwerck I et al. Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species. Int J Syst Evol Microbiol 2007; 57: 2952– 2959 [CrossRef] [PubMed]
-
16. Snauwaert I, Papalexandratou Z, de Vuyst L, Vandamme P. Characterization of strains of Weissella fabalis sp. nov. and Fructobacillus tropaeoli from spontaneous cocoa bean fermentations. Int J Syst Evol Microbiol 2013; 63: 1709– 1716 [CrossRef] [PubMed]
-
17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28: 2731– 2739 [CrossRef] [PubMed]
-
18. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004; 101: 11030– 11035 [CrossRef] [PubMed]
-
19. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110: 1281– 1286 [CrossRef] [PubMed]
-
20. Harrigan WF. Laboratory methods in food microbiology, 3rd ed. San Diego, CA: Academic Press; 1998
-
21. Claus D. A standardized Gram staining procedure. World J Microbiol Biotechnol 1992; 8: 451– 452 [CrossRef] [PubMed]
-
22. Lopes MF, Pereira CI, Rodrigues FM, Martins MP, Mimoso MC et al. Registered designation of origin areas of fermented food products defined by microbial phenotypes and artificial neural networks. Appl Environ Microbiol 1999; 65: 4484– 4489 [PubMed]
-
23. Tjandraatmadja M, Norton BW, Rae ICM. A numerical taxonomic study of lactic acid bacteria from tropical silages. J Appl Bacteriol 1990; 68: 543– 553 [CrossRef]
-
24. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Beveridge JAB TJ, Marzluf GA, Schmidt TM, Snyder LR. (editors) Methods for General and Molecular Microbiology Washington, DC: ASM Press; 2007; pp. 330– 393
-
25. Ma S, Huang Y, Wang C, Fan H, Dai L et al. Defluviitalea raffinosedens sp. nov., a thermophilic, anaerobic, saccharolytic bacterium isolated from an anaerobic batch digester treating animal manure and rice straw. Int J Syst Evol Microbiol 2017; 67: 1607– 1612 [CrossRef] [PubMed]
-
26. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996; 42: 989– 1005 [CrossRef]
-
27. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988; 38: 358– 361 [CrossRef]
-
28. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16: 584– 586 [PubMed]
-
29. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66: 199– 202 [CrossRef]
-
30. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13: 128– 130 [CrossRef]

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