- Volume 61, Issue 12, 2011
Volume 61, Issue 12, 2011
- Notification List
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Notification that new names and new combinations have appeared in volume 61, part 9, of the IJSEM
This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed according to the Rules of priority (i.e. page number and order of valid publication of names in the original articles). Taxonomic opinions included in this List (i.e. the creation of synonyms or the emendation of circumscriptions) cannot be considered as validly published nor, in any other way, approved by the International Committee on Systematics of Prokaryotes and its Judicial Commission.
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- New Taxa
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- Actinobacteria
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Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential
More LessA Gram-positive-staining, aerobic, non-motile, coccoid shaped, halotolerant bacterium (strain JG 06T) was isolated from the roots of Salicornia brachiata, an extreme halophyte. Phylogenetic analysis based on 16S rRNA gene sequence showed that the novel strain had sequence similarities of 99.2 % to Brachybacterium paraconglomeratum JCM 11608T, 99.0 % to Brachybacterium conglomeratum DSM 10241 and 98.2 % to Brachybacterium faecium DSM 4810T. DNA–DNA hybridization with B. paraconglomeratum DSM 46341T, B. conglomeratum DSM 10241T, B. faecium DSM 4810T, Brachybacterium tyrofermentans DSM 10673T, Brachybacterium alimentarium DSM 10672T, Brachybacterium fresconsis DSM 14564T, Brachybacterium sacelli DSM 14566T and Brachybacterium muris DSM 15460T resulted in reassociation values of 36.2 %, 36.5 %, 35.8 %, 27.6 %, 27.9 %, 28.2 %, 28.7 % and 11.2 %, respectively. The peptidoglycan type of strain JG 06T was variant A4γ. The menaquinone content was MK7 (100 %). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl diglyceride, three unidentified phospholipids and three glycolipids. The predominant fatty acid was anteiso-C15 : 0 (52.07 %); significant amounts of iso-C16 : 0 (12.38 %), iso-C15 : 0 (8.59 %) and anteiso-C17 : 0 (10.03 %) were also present. The G+C content of the DNA was 73.0 mol%. The strain formed a growth pellicle in nitrogen-free semisolid NFb medium containing NaCl at levels of up to 4 % (w/v) and reduced acetylene to ethylene, a result indicative of N2 fixation. In nutrient broth medium the novel strain grew at NaCl concentrations up to 15 % (w/v). It also had the ability to produce indole-3-acetic acid (IAA) and siderophores, utilized 1-aminocyclopropane-1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. On the basis of physiological, biochemical data and phylogenetic analyses, strain JG 06T should be placed in the genus Brachybacterium. Strain JG 06T represents a novel species of the genus Brachybacterium for which the name Brachybacterium saurashtrense sp. nov. is proposed (type strain JG 06T = DSM 23186T = IMCC 252T).
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Spinactinospora alkalitolerans gen. nov., sp. nov., an actinomycete isolated from marine sediment
More LessA novel marine actinomycete, designated CXB654T, was isolated from marine sediment collected at a depth of 17.5 m near the Yellow Sea Cold Water Mass, China. Optimal growth occurred at 37.0 °C, at pH 7.0–8.0 and in 3–8 % (w/v) NaCl. Strain CXB654T formed branched substrate mycelium without fragmentation. Abundant aerial mycelium differentiated into long or short chains of spores and spores were elliptical and cylindrical with spiny surfaces. Strain CXB654T contained meso-diaminopimelic acid as the diagnostic diamino acid, and ribose and glucose as the major whole-cell components. Phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. MK-10(H8), MK-10(H6) and MK-9(H8) were the predominant menaquinones. The major fatty acids were i-C16 : 0 (24.46 %), ai-C17 : 0 (20.66 %) and C18 : 0 (20.14 %). The DNA G+C content was 71.1 mol%. Comparative analysis of 16S rRNA gene sequences showed that the novel strain was most closely related to genera within the family Nocardiopsaceae, but formed a separate lineage. Highest sequence similarities were to Murinocardiopsis flavida DSM 45312T (96.6 %), Thermobifida halotolerans YIM 90462T (96.5 %) and Marinactinospora thermotolerans DSM 45154T (96.1 %). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain CXB654T was considered to represent a novel species in a new genus in the family Nocardiopsaceae, and the name Spinactinospora alkalitolerans gen. nov., sp. nov. is proposed; the type strain is CXB654T ( = DSM 45414T = LMG 25485T).
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Streptomyces fenghuangensis sp. nov., isolated from seawater
More LessAn actinomycete, designated strain GIMN4.003T, was isolated from seawater collected in Sanya, China. It produced white aerial mycelium and yellow substrate mycelium on Gause’s synthetic agar medium no. 1. The substrate mycelium colour was not sensitive to pH. Scanning electron microscopy observations revealed that GIMN4.003T produced straight to flexuous spore chains of rough to warty spores. ll-Diaminopimelic acid was present in the cell-wall hydrolysate. Based on chemotaxonomy and morphological features, strain GIMN4.003T was identified as a member of the genus Streptomyces. Melanin was not produced. No antimicrobial activity was detected against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Penicillium citrinum or Candida albicans. Analysis of the 16S rRNA gene sequence revealed that the highest sequence similarity was to Streptomyces radiopugnans R97T (99.0 %). However, DNA relatedness between GIMN4.003T and S. radiopugnans DSM 41901T was low (41.24±1.47 %). Furthermore, the morphological, physiological and biochemical characteristics of strain GIMN4.003T were different from those of S. radiopugnans DSM 41901T and the type strains of other closely related Streptomyces species. On the basis of its physiological and molecular properties, it is evident that strain GIMN4.003T ( = CCTCCM 208215T = NRRL B-24801T) represents the type strain of a novel species within the genus Streptomyces, for which the name Streptomyces fenghuangensis sp. nov. is proposed.
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Micrococcus lactis sp. nov., isolated from dairy industry waste
More LessA Gram-positive, yellow-pigmented, actinobacterial strain, DW152T, was isolated from a dairy industry effluent treatment plant. 16S rRNA gene sequence analysis indicated that strain DW152T exhibited low similarity with many species with validly published names belonging to the genera Micrococcus and Arthrobacter. However, phenotypic properties including chemotaxonomic markers affiliated strain DW152T to the genus Micrococcus. Strain DW152T had ai-C15 : 0 and i-C15 : 0 as major cellular fatty acids, and MK-8(H2) as the major menaquinone. The cell-wall peptidoglycan of strain DW152T had l-lysine as the diagnostic amino acid and the type was A4α. The DNA G+C content of strain DW152T was 68.0 mol%. In 16S rRNA gene sequence analysis, strain DW152T exhibited significant similarity with Micrococcus terreus NBRC 104258T, but the mean value of DNA–DNA relatedness between these strains was only 42.3 %. Moreover, strain DW152T differed in biochemical and chemotaxonomic characteristics from M. terreus and other species of the genus Micrococcus. Based on the above differences, we conclude that strain DW152T should be treated as a novel species of the genus Micrococcus, for which the name Micrococcus lactis sp. nov. is proposed. The type strain of Micrococcus lactis sp. nov. is DW152T ( = MTCC10523T = DSM 23694T).
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Sphaerisporangium krabiense sp. nov., isolated from soil
A Gram-staining-positive, filamentous bacterial strain, designated A-T 0308T, was isolated from soil of a tropical mangrove forest in Thailand. Strain A-T 0308T developed spherical sporangia containing non-motile spores on aerial mycelium. The novel strain contained meso-diaminopimelic acid, N-acetyl-type peptidoglycan and madurose, mannose, ribose, galactose and glucose as whole-cell sugars. The predominant menaquinones were MK-9(H4) and MK-9(H6); a small amount of MK-9(H2) and MK-9 was also detected. Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and phosphoglycolipid. The predominant cellular fatty acids were iso-C16 : 0 and 10-methylated C17 : 0. The G+C content of the DNA was 72 mol%. Phenotypic and chemotaxonomic analyses showed that the novel isolate had characteristics typical of members of the genus Sphaerisporangium. 16S rRNA gene sequence analysis also indicated that the strain belongs to the genus Sphaerisporangium and that it represents a clade distinct from other members of the genus with sequence similarities ranging from 96.3 to 97.8 % between the novel strain and its closest relatives. Based on the results of phenotypic, chemotaxonomic and phylogenetic studies, strain A-T 0308T ( = BCC 21702T = NBRC 107571T) represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium krabiense sp. nov. is proposed.
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Descriptions of Actinoplanes ianthinogenes nom. rev. and Actinoplanes octamycinicus corrig. comb. nov., nom. rev.
More LessPhylogenetic analysis of ‘Actinoplanes ianthinogenes’ Coronelli et al. 1974 and ‘Actinoplanes ianthinogenes subsp. octamycini’ Gauze et al. 1979 based on 16S rRNA gene sequencing data revealed that these organisms form a clade in the family Micromonosporaceae. Morphological and chemotaxonomic characteristics of strains of these species were consistent with those of members of the genus Actinoplanes. Morphological, DNA–DNA hybridization, physiological, biochemical and chemotaxonomic data showed that ‘A. ianthinogenes’ and ‘A. ianthinogenes subsp. octamycini’ can be easily differentiated from each other and that they merit separate species status. On the basis of morphological, physiological, biochemical, chemotaxonomic and DNA–DNA hybridization data, it is concluded that ‘A. ianthinogenes’ and ‘A. ianthinogenes subsp. octamycini’ should be assigned the status of two novel species: Actinoplanes ianthinogenes nom. rev. (type strain NBRC 13996T = A/1668T = ATCC 21884T = BCRC 13611T = DSM 43864T = IMSNU 20032T = JCM 3249T = KCTC 9347T = KCTC 9592T = NCIMB 12639T = NRRL B-16720T) and Actinoplanes octamycinicus corrig. comb. nov., nom. rev. (type strain NBRC 14524T = INA 4041T = ATCC 43632T = JCM 9649T = KCTC 9593T), respectively.
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Actinophytocola xinjiangensis sp. nov., isolated from virgin forest soil
More LessAn aerobic, non-motile actinobacterium, strain QAIII60T, was isolated from virgin forest soil of Kanas Nature Reserve, Xinjiang, north-west China. The isolate produced a very scant aerial mycelium that fragmented into cylindrical spores and a non-fragmented substrate mycelium with occasional septa. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose, galactose, glucose, ribose and rhamnose (trace). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. The major cellular fatty acids were iso-C16 : 0, iso-C14 : 0, iso-C16 : 1 H and C17 : 1ω6c. The isoprenoid quinones consisted of MK-9(H4) and MK-10(H2). The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QAIII60T formed a distinct phyletic line that was most closely, albeit loosely, associated with the genus Actinophytocola. A number of physiological characteristics differentiated the isolate from members of the genus Actinophytocola. On the basis of these data, we propose that strain QAIII60T ( = CGMCC 4.4663T = NBRC 106673T) be assigned as the type strain of a novel species, Actinophytocola xinjiangensis sp. nov.
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Nocardia artemisiae sp. nov., an endophytic actinobacterium isolated from a surface-sterilized stem of Artemisia annua L.
More LessA novel actinobacterium, designated YIM 65623T, was isolated from a surface-sterilized stem of Artemisia annua L. Strain YIM 65623T had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus Nocardia. Growth occurred with 0–7 % (w/v) NaCl (optimum 0–3 %), at pH 5.0–9.0 (optimum pH 6.0) and at 10–37 °C (optimum 20–28 °C). Comparative 16S rRNA gene sequence analysis showed that strain YIM 65623T constituted a distinct sublineage within the genus Nocardia and displayed 94.1–98.2 % sequence similarity to members of established species in the genus Nocardia. However, DNA–DNA relatedness and physiological and biochemical characteristics showed that strain YIM 65623T could be differentiated from its closest phylogenetic relatives. The G+C content of the genomic DNA was 69.6 mol%. It is proposed that strain YIM 65623T be classified as a representative of a novel species, Nocardia artemisiae sp. nov. The type strain is YIM 65623T ( = DSM 45379T = CCTCC AA 209038T).
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Phytomonospora endophytica gen. nov., sp. nov., isolated from the roots of Artemisia annua L.
More LessA novel endophytic actinomycete, strain YIM 65646T, was isolated from the roots of Artemisia annua L. collected from Yunnan Province, south-west China. Growth was observed in the temperature range 10–40 °C (optimum 20–28 °C) and at pH 6.0–9.0 (optimum pH 7.0). The organism formed well-developed, branched substrate mycelia, but aerial mycelium was not produced. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain YIM 65646T belonged to the family Micromonosporaceae (sharing ≤93.6 % sequence similarity with members of this family) and formed a distinct clade in the Micromonosporaceae phylogenetic tree. The strain contained meso-diaminopimelic acid in the cell wall and mannose, ribose, galactose and glucose in whole-cell hydrolysates. The major menaquinones were MK-10(H4), MK-10(H2), MK-8(H2), MK-9(H2) and MK-10(H6). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, iso-C17 : 0 and iso-C16 : 0. The DNA G+C content of strain YIM 65646T was 70.0 mol%. On the basis of morphological and chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain should be classified in a novel genus and species, Phytomonospora endophytica gen. nov., sp. nov., in the family Micromonosporaceae. The type strain is YIM 65646T ( = CCTCC AA 209041T = DSM 45386T).
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Frondihabitans cladoniiphilus sp. nov., an actinobacterium of the family Microbacteriaceae isolated from lichen, and emended description of the genus Frondihabitans
More LessA novel actinobacterium, designated strain CafT13T, was isolated from the thallus of the reindeer lichen Cladonia arbuscula sampled in the Austrian Alps (Koralpe). The organism was aerobic, with rod- to irregular-shaped cells (often forming dense clusters of cells when grown in liquid medium), Gram-stain-positive, oxidase-negative, catalase-positive and non-motile. It was able to grow at 1 °C and at low to neutral pH, but not above 30 °C or at high pH. The peptidoglycan type was B2β with ornithine as the diagnostic diamino acid. The menaquinones were MK-7 and MK-8. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids and one unidentified aminolipid. The predominant fatty acids were C18 : 1, C14 : 0 2-OH, C17 : 1ω9c, C16 : 0 and anteiso-C15 : 0. The mean DNA G+C content of strain CafT13T was 69.0±0.17 mol%. 16S rRNA gene sequence analysis showed that strain CafT13T belongs to the family Microbacteriaceae, within the genus Frondihabitans. The mean level of DNA–DNA relatedness between strain CafT13T and the type strain of Frondihabitans australicus was 35.2±5.23 %. The enzyme spectrum of strain CafT13T differentiated it from recognized species of the genus Frondihabitans. Based on molecular, chemotaxonomic and physiological data, strain CafT13T is considered to represent a novel species of the genus Frondihabitans, for which the name Frondihabitans cladoniiphilus sp. nov. is proposed; the type strain is CafT13T ( = DSM 23273T = LMG 25550T).
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- Proteobacteria
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Proposal that the arsenite-oxidizing organisms Thiomonas cuprina and ‘Thiomonas arsenivorans’ be reclassified as strains of Thiomonas delicata, and emended description of Thiomonas delicata
The three As(III)-oxidizing members of the class Betaproteobacteria Thiomonas delicata, Thiomonas cuprina and ‘Thiomonas arsenivorans’ were isolated from mining sites in geographically distinct areas, namely Japan, Germany and France, respectively. They are all able to oxidize As(III) but only ‘T. arsenivorans’ and T. cuprina show efficient autotrophic growth with As(III) and are able to grow on a sole carbon source. These two organisms are also motile, whereas T. delicata is not. Only T. cuprina can grow autotrophically on chalcopyrite. The three strains share >99 % gene sequence similarity with each other based on their 16S rRNA genes and 16S–23S ITS regions. DNA–DNA hybridization results are above, or close to, the threshold value of 70 % recommended for the definition of bacterial species. The three taxa show very similar fatty acid profiles with differences only in five minor fatty acid components. They possess phylogenetic and chemotaxonomic similarities supporting the reclassification of these taxa as a single species. We propose that ‘T. arsenivorans’ and T. cuprina be reassigned as strains of T. delicata (type strain DSM 17897T).
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Acinetobacter rudis sp. nov., isolated from raw milk and raw wastewater
Two bacterial strains, G30T and A1PC16, isolated respectively from raw milk and raw wastewater, were characterized using a polyphasic approach. Chemotaxonomic characterization supported the inclusion of these strains in the genus Acinetobacter, with Q-8 and Q-9 as the major respiratory quinones, genomic DNA G+C contents within the range observed for this genus (38–47 mol%) and C16 : 0, C18 : 1ω9c and C16 : 1ω7c/iso-C15 : 0 2-OH as the predominant fatty acids. The observation of 16S rRNA gene sequence similarity lower than 97 % with other Acinetobacter species with validly published names led to the hypothesis that these isolates could represent a novel species. This hypothesis was supported by comparative analysis of partial sequences of the genes rpoB and gyrB, which showed that strains G30T and A1PC16 did not cluster with any species with validly published names, forming a distinct lineage. DNA–DNA hybridizations confirmed that the two strains were members of the same species, which could be distinguished from their congeners by several phenotypic characteristics. On the basis of these arguments, it is proposed that strains G30T and A1PC16 represent a novel species, for which the name Acinetobacter rudis sp. nov. is proposed. The type strain is strain G30T ( = LMG 26107T = CCUG 57889T = DSM 24031T = CECT 7818T).
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Olivibacter oleidegradans sp. nov., a hydrocarbon-degrading bacterium isolated from a biofilter clean-up facility on a hydrocarbon-contaminated site
A novel hydrocarbon-degrading, Gram-negative, obligately aerobic, non-motile, non-sporulating, rod-shaped bacterium, designated strain TBF2/20.2T, was isolated from a biofilter clean-up facility set up on a hydrocarbon-contaminated site in Hungary. It was characterized by using a polyphasic approach to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate is affiliated with the genus Olivibacter in the family Sphingobacteriaceae. It was found to be related most closely to Olivibacter ginsengisoli Gsoil 060T (93.3 % 16S rRNA gene sequence similarity). Strain TBF2/20.2T grew at pH 6–9 (optimally at pH 6.5–7.0) and at 15–42 °C (optimally at 30–37 °C). The major fatty acids were iso-C15 : 0 (39.4 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c; 26.0 %), iso-C17 : 0 3-OH (14.5 %) and C16 : 0 (4.5 %). The major menaquinone was MK-7 and the predominant polar lipid was phosphatidylethanolamine. The DNA G+C content of strain TBF2/20.2T was 41.2 mol%. Physiological and chemotaxonomic data further confirmed the distinctiveness of strain TBF2/20.2T from recognized members of the genus Olivibacter. Thus, strain TBF2/20.2T is considered to represent a novel species of the genus Olivibacter, for which the name Olivibacter oleidegradans sp. nov. is proposed. The type strain is TBF2/20.2T ( = NCAIM B 02393T = CCM 7765T).
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Catenovulum agarivorans gen. nov., sp. nov., a peritrichously flagellated, chain-forming, agar-hydrolysing gammaproteobacterium from seawater
More LessA novel Gram-negative, strictly aerobic, agar-hydrolysing bacterium, designated YM01T, was isolated from seawater samples collected from the Yellow Sea (coastal region of Qingdao, PR China). Cells were rod-shaped, peritrichously flagellated and formed long chains end-to-end. The isolate had an absolute requirement for Na+ ions, but not seawater, for growth and grew optimally at about 28 °C, in 2 % NaCl and at pH 8.0–9.0. The isolate could not be cultured in marine broth 2216, but grew well on marine agar 2216. YM01T was able to hydrolyse cellulose, starch, aesculin and Tween 80, but not egg yolk, gelatin, urea or casein. 16S rRNA gene sequence analysis demonstrated that this isolate was unique, showing only 88.4–91.0 % sequence similarity to its closest neighbours, including members of the genera Glaciecola (88.4–91.0 %), Alteromonas (88.7–89.6 %), Aestuariibacter (89.3–90.4 %), Salinimonas (89.0 %), Bowmanella (90.1–90.3 %) and Agarivorans (88.5–89.9 %). Phylogenetic analyses demonstrated that strain YM01T formed a distinct clade closely related to species of the family Alteromonadaceae within the group of Alteromonas-like gammaproteobacteria. It contained menaquinone MK-7 as the predominant isoprenoid quinone and C16 : 0 (38.3 %), C16 : 1ω7c and/or iso-C15 : 0 2-OH (29.0 %), C18 : 1ω7c (9.3 %) and C10 : 0 3-OH (8.2 %) as major cellular fatty acids. Phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid were the major phospholipid constituents. The DNA G+C content was 44.8 mol%. Based on its phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain YM01T is considered to represent a novel species in a new genus in the Gammaproteobacteria, for which the name Catenovulum agarivorans gen. nov., sp. nov. is proposed; the type strain of Catenovulum agarivorans is YM01T ( = CGMCC 1.10245T = DSM 23111T = JCM 16580T).
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Oceanisphaera ostreae sp. nov., isolated from seawater of an oyster farm, and emended description of the genus Oceanisphaera Romanenko et al. 2003
More LessA Gram-stain-negative, motile, non-spore-forming and short rod- or rod-shaped bacterial strain, T-w6T, was isolated from seawater of an oyster farm in the South Sea, Korea. Strain T-w6T grew optimally at 25 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain T-w6T joined the cluster comprising Oceanisphaera species with a bootstrap resampling value of 90.8 %, and this cluster joined the clade comprising members of the genera Oceanimonas and Zobellella with a bootstrap resampling value of 100 %. Strain T-w6T exhibited 16S rRNA gene sequence similarity of 95.9 and 96.6 % to the type strains of Oceanisphaera litoralis and Oceanisphaera donghaensis, respectively. Strain T-w6T and the type strains of Oceanisphaera litoralis and Oceanisphaera donghaensis had Q-8 as the predominant ubiquinone and iso-C15 : 0 2-OH and/or C16 : 1ω7c, C18 : 1ω7c and C16 : 0 as the major fatty acids. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain T-w6T was 56.6 mol%. Mean DNA–DNA relatedness of strain T-w6T with Oceanisphaera litoralis DSM 15406T and Oceanisphaera donghaensis KCTC 12522T was 13 and 10 %, respectively. Phenotypic properties of strain T-w6T demonstrated that this strain could be distinguished from the other Oceanisphaera species. On the basis of the data presented, strain T-w6T is considered to represent a novel species of the genus Oceanisphaera, for which the name Oceanisphaera ostreae sp. nov. is proposed; the type strain is T-w6T ( = KCTC 23422T = CCUG 60525T). An emended description of the genus Oceanisphaera is also presented.
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Description of Spongiibacter borealis sp. nov., isolated from Arctic seawater, and reclassification of Melitea salexigens Urios et al. 2008 as a later heterotypic synonym of Spongiibacter marinus Graeber et al. 2008 with emended descriptions of the genus Spongiibacter and Spongiibacter marinus
More LessA Gram-negative, rod-shaped and motile strain, designated CL-AS9T, was isolated from polar seawater of the Arctic. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Spongiibacter, sharing 93.9 % and 93.7 % sequence similarities with the type strains of Spongiibacter tropicus CL-CB221T and Spongiibacter marinus HAL40bT, respectively. Phylogenetic analyses revealed that strain CL-AS9T formed a separate branch that was distinct from a clade comprising Spongiibacter marinus HAL40bT, Spongiibacter tropicus CL-CB221T and Melitea salexigens 5IX/A01/131T. Cells of the strain grew optimally at 20–25 °C and pH 6.6–8.0 in the presence of 3–4 % (w/v) sea salts. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified aminophospholipid. The major quinone was ubiquinone 8. The major cellular fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH (23.1 %), C17 : 1ω8c (22.1 %) and C18 : 1ω7c (15.6 %). The genomic DNA G+C content was 53.6 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, we propose the name Spongiibacter borealis sp. nov. with the type strain CL-AS9T ( = KCCM 90094T = JCM 17304T) and the reclassification of Melitea salexigens as a later heterotypic synonym of Spongiibacter marinus. We also provide emended descriptions of the genus Spongiibacter and Spongiibacter marinus.
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Pusillimonas harenae sp. nov., isolated from a sandy beach, and emended description of the genus Pusillimonas
A Gram-stain-negative, motile bacterium with two lateral flagella, designated strain B201T, was isolated from beach sand from the Taean coast in South Korea. Cells were ovoid rods and positive for catalase and oxidase. Growth of strain B201T was observed between 15 and 45 °C (optimum, 30 °C) and between pH 5.0 and 9.0 (optimum, pH 6.0–7.5). Strain B201T contained ubiquinone Q-8 as the major isoprenoid quinone, but MK-6 was also present as a minor quinone. The major fatty acids of strain B201T were C17 : 0 cyclo, C16 : 0, summed feature 2 (iso-C16 : 1 I/C14 : 0 3-OH and/or C12 : 0 ALDE), C12 : 0 and C19 : 0 cyclo ω8c. The major cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and three aminolipids. The genomic DNA G+C content was 53.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a phyletic lineage with Pusillimonas ginsengisoli DCY25T within the genus Pusillimonas. Strain B201T was most closely related to P. ginsengisoli DCY25T and Pusillimonas soli MJ07T with similarities of 98.6 and 97.5 %, respectively. However, DNA–DNA relatedness values of strain B201T with P. ginsengisoli DCY25T and P. soli MJ07T were 30.2±5.4 and 4.9±1.8 %, respectively. On the basis of chemotaxonomic data and molecular properties, strain B201T represents a novel species of the genus Pusillimonas, for which the name Pusillimonas harenae sp. nov. is proposed; the type strain is B201T ( = KACC 14927T = JCM 16917T). An emended description of the genus Pusillimonas is given.
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Bradyrhizobium cytisi sp. nov., isolated from effective nodules of Cytisus villosus
More LessSeveral strains isolated from Cytisus villosus nodules have been characterized based on their diverse genetic, phenotypic and symbiotic characteristics. According to 16S rRNA gene sequence analysis, the isolates formed a group that was closely related to Bradyrhizobium canariense BTA-1T with 99.4 % similarity. Analysis of three housekeeping genes, recA, atpD and glnII, suggested that the C. villosus strains represent a novel Bradyrhizobium species most closely related to B. canariense BTA-1T with similarities of 94.2, 96.7 and 94.5 %, respectively. All these differences were congruent with DNA–DNA hybridization analysis, which revealed 31 % relatedness between a representative strain (CTAW11T) isolated from C. villosus nodules and B. canariense BTA-1T. Phenotypic differences among the strains isolated from C. villosus and B. canariense were based on assimilation of carbon and nitrogen sources. The nodC and nifH genes of strain CTAW11T were phylogenetically related to those of strains belonging to bv. genistearum and divergent from those of bv. glycinearum and, accordingly, they do not nodulate soybean. Based on the genotypic and phenotypic data obtained in this study, our strains should be classified as representatives of a novel species for which the name Bradyrhizobium cytisi sp. nov. is proposed; the type strain is CTAW11T ( = LMG 25866T = CECT 7749T).
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Altererythrobacter ishigakiensis sp. nov., an astaxanthin-producing bacterium isolated from a marine sediment
A Gram-negative, non-motile, non-spore-forming, halophilic rod, designated JPCCMB0017T, was isolated from a marine sediment of the coastal area of Okinawa, Japan. The isolate formed orange–red colonies on marine agar. Bacteriochlorophyll α was absent and sphingoglycolipid 1 and other carotenoids, including astaxanthin, adonixanthin and zeaxanthin, were present. Ubiquinone-10 (Q-10) was the main respiratory quinone and C18 : 1ω7c was the major cellular fatty acid. The G+C content of DNA was 59.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus Altererythrobacter in the family Erythrobacteraceae. Strain JPCCMB0017T exhibited 96.8 % 16S rRNA gene sequence similarity with Altererythrobacter marinus H32T. Unlike other members of the genus Altererythrobacter, strain JPCCMB0017T reduced nitrate. On the basis of genotypic and phenotypic data, a novel species is proposed to accommodate this isolate, with the name Altererythrobacter ishigakiensis sp. nov. The type strain is JPCCMB0017T ( = NITE-AP48T = ATCC BAA-2084T = NBRC 107699T).
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Pseudomonas composti sp. nov., isolated from compost samples
Two unusual, Gram-negative, catalase- and oxidase-positive rods, designated C2T and C5, were isolated from compost samples. Comparative 16S rRNA gene sequencing studies demonstrated that both isolates were members of the genus Pseudomonas and belonged to the Pseudomonas aeruginosa group. Strain C2T was most closely related to Pseudomonas cuatrocienegasensis 1NT and Pseudomonas borbori R-20821T (97.9 and 97.8 % 16S rRNA gene sequence similarity, respectively). However, phylogenetic analysis based on rpoD gene sequences revealed that both isolates could be discriminated from members of the P. aeruginosa group that exhibited >97 % 16S rRNA gene sequence similarity. The DNA G+C content of strain C2T was 61.5 mol%. The major fatty acids of strain C2T were a summed feature (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C18 : 1ω7c/12t/9t, C16 : 0 and C12 : 0, which supported the isolates’ affiliation with the genus Pseudomonas. Moreover, strain C2T could be distinguished from its closest phylogenetic neighbours of the genus Pseudomonas by DNA–DNA hybridization studies and biochemical tests. On the basis of both phenotypic and phylogenetic findings, it is proposed that the isolates be classified as a novel species, with the name Pseudomonas composti sp. nov. The type strain is C2T ( = CECT 7516T = CCUG 59231T).
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Marinobacter daqiaonensis sp. nov., a moderate halophile isolated from a Yellow Sea salt pond
More LessA Gram-negative, oxidase- and catalase-positive, moderately halophilic strain, designated YCSA40T, was isolated from sediment of Daqiao saltern in Qingdao, on the east coast of China. Growth occurred at 10–45 °C, at pH 5–9 and with 1–15 % NaCl. Strain YCSA40T showed the highest 16S rRNA gene sequence similarity to Marinobacter segnicrescens SS011B1-4T (97 %) and M. gudaonensis SL014B61AT (96.9 %) and 16S rRNA gene sequence phylogenetic analysis assigned the isolate to the genus Marinobacter. Strain YCSA40T contained C18 : 1ω9c (34.8 %), C16 : 0 (11.6 %), C19 : 0 cyclo ω10c/C19 : 1ω6c (10.5 %), C16 : 1ω9c (8.4 %), C17 : 0 (6.3 %) and C12 : 0 3-OH (5.8 %) as the predominant fatty acids. The DNA G+C content was 60.8 mol% and the major ubiquinone was Q-9. These chemotaxonomic characters were all consistent with membership of the genus Marinobacter. DNA–DNA relatedness between the isolate and M. segnicrescens CGMCC 1.6489T, M. gudaonensis CGMCC 1.6294T and other type strains of species of the genus Marinobacter was ≤30 %. On the basis of the aforementioned data, it was concluded that strain YCSA40T represents a novel species of the genus Marinobacter, for which the name Marinobacter daqiaonensis sp. nov. is proposed. The type strain is YCSA40T ( = CGMCC 1.9167T = NCCB 100308T = LMG 25365T).
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Vibrio variabilis sp. nov. and Vibrio maritimus sp. nov., isolated from Palythoa caribaeorum
Two novel vibrio isolates (R-40492T and R-40493T) originating from the zoanthid Palythoa caribaeorum in Brazil in 2005 were taxonomically characterized by means of a polyphasic approach comprising multilocus sequence analysis (MLSA), DNA–DNA hybridization (DDH), ΔT m analysis and phenotypic characterization. Phylogenetic analysis based on 16S rRNA gene sequences showed that R-40492T and R-40493T fell within the genus Vibrio and were most closely related to each other with 99 % similarity; similarities of these two novel isolates towards Vibrio neptunius LMG 20536T, Vibrio coralliilyticus LMG 20984T, Vibrio nigripulchritudo LMG 3896T, Vibrio sinaloensis LMG 25238T and Vibrio brasiliensis LMG 20546T varied between 97.1 and 98.5 %. DDH experiments showed that the two isolates had less than 15 % relatedness to the phylogenetically most closely related Vibrio species. R-40492T and R-40493T had 55–57 % relatedness to each other. The ΔTm between R-40492T and R-40493T was 6.12 °C. In addition, MLSA of concatenated sequences (16S rRNA, ftsZ, gyrB, recA, rpoA, topA, pyrH and mreB; 6035 bp in length) showed that the two novel isolates formed a separate branch with less than 92 % concatenated gene sequence similarity towards known species of vibrios. Two novel species are proposed to accommodate these novel isolates, namely Vibrio variabilis sp. nov. (type strain, R-40492T = LMG 25438T = CAIM 1454T) and Vibrio maritimus sp. nov. (type strain, R-40493T = LMG 25439T = CAIM 1455T).
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Proposal of Bisgaardia hudsonensis gen. nov., sp. nov. and an additional genomospecies, isolated from seals, as new members of the family Pasteurellaceae
More LessPhenotypic and phylogenetic studies were performed on eight Gram-negative-staining, rod-shaped bacteria isolated from seals. Biochemical and physiological studies showed identical profiles for all of the isolates and indicated that they were related to the family Pasteurellaceae. 16S rRNA gene sequencing demonstrated that the organism represented a distinct cluster with two sublines within the family Pasteurellaceae with <96 % sequence similarity to any recognized species. Multilocus sequence analysis (MLSA) including rpoB, infB and recN genes further confirmed these findings with the eight isolates forming a genus-like cluster with two branches. Genome relatedness as deduced from recN gene sequences suggested that the isolates represented a new genus with two species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that these bacteria from seals are classified as Bisgaardia hudsonensis gen. nov., sp. nov. (the type species) and Bisgaardia genomospecies 1. The G+C content of the DNA was 39.5 mol%. The type strain of Bisgaardia hudsonensis gen. nov., sp. nov. is M327/99/2T ( = CCUG 43067T = NCTC 13475T = 98-D-690BT) and the reference strain of Bisgaardia genomospecies 1 is M1765/96/5 ( = CCUG 59551 = NCTC 13474).
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The genus Listonella MacDonell and Colwell 1986 is a later heterotypic synonym of the genus Vibrio Pacini 1854 (Approved Lists 1980) – a taxonomic opinion
We analysed the taxonomic position of the genus Listonella based on phylogenetic, genomic and phenotypic data. The species of the genus Listonella were nested within the genus Vibrio according to the 16S rRNA gene sequence-based phylogenetic tree. The closest neighbour of Vibrio (Listonella) anguillarum strains LMG 4437T and ATCC 68554 ( = strain 775) was Vibrio ordalii LMG 13544T, with more than 99.5 % 16S rRNA gene sequence similarity. Furthermore, Vibrio (Listonella) pelagius is highly related to Vibrio splendidus. According to average amino acid identity (AAI), multilocus sequence analysis (MLSA) and Karlin genome signature, the closest neighbour of L. anguillarum ATCC 68554 is V. ordalii LMG 13544T, with 95 % AAI, 98 % MLSA and 5 in Karlin. V. anguillarum ATCC 68554 and Vibrio cholerae N16961 had 77 % similarity in AAI, 85 % in MLSA and 14 in the Karlin signature. Phenotypic analyses of previously published data for V. (L.) anguillarum and V. (L.) pelagius revealed that the genus Listonella is extremely similar to the genus Vibrio. V. ordalii and L. anguillarum strains yielded up to 67 % DNA–DNA hybridization. There are only a few phenotypic features that might be used to discriminate these two species: L. anguillarum is positive for the Voges–Proskauer reaction, citrate utilization, starch hydrolysis, lipase activity and acid production from glycerol, sorbitol and trehalose, whereas V. ordalii is negative for these traits. We suggest that the genus Listonella is a later heterotypic synonym of the genus Vibrio and propose to use the names Vibrio anguillarum and Vibrio pelagius in place of Listonella anguillarum and Listonella pelagia, respectively.
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Rhodanobacter panaciterrae sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a ginseng field
More LessA novel gammaproteobacterium, designated LnR5-47T, was isolated from soil of a ginseng field in Liaoning province, China. The isolate was a Gram-negative, aerobic, non-motile, non-spore-forming rod. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LnR5-47T belonged to the genus Rhodanobacter. The isolate was most closely related to Rhodanobacter ginsengisoli GR17-7T, Rhodanobacter terrae GP18-1T, Dyella ginsengisoli Gsoil 3046T, Rhodanobacter soli DCY45T, Dyella soli JS12-10T and Dyella japonica IAM 15069T (98.0, 97.9, 97.7, 97.3, 97.2 and 97.1 % 16S rRNA gene sequence similarity, respectively). Chemotaxonomic data (Q-8 as the predominant ubiquinone, and iso-C16 : 0, iso-C17 : 1ω9c and iso-C15 : 0 as the major fatty acids) also supported the affiliation of strain LnR5-47T with the genus Rhodanobacter. However, DNA–DNA relatedness between strain LnR5-47T and its closest phylogenetic neighbours was <25.8 %. Moreover, physiological and biochemical tests phenotypically differentiated the isolate from other members of the genus Rhodanobacter. Therefore, strain LnR5-47T represents a novel species, for which the name Rhodanobacter panaciterrae sp. nov. is proposed; the type strain is LnR5-47T ( = KACC 12826T = KCTC 22232T = LMG 24460T).
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- Other Bacteria
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‘Candidatus Phytoplasma costaricanum’ a novel phytoplasma associated with an emerging disease in soybean (Glycine max)
More LessA novel phytoplasma, designated strain SoyST1c1, associated with a newly emerging disease in soybean (Glycine max), known as soybean stunt (SoyST), was found in 2002 in a soybean plantation in Alajuela Province, Costa Rica. The same phytoplasma, or a very closely related strain, also infected sweet pepper (Capsicum annuum) with purple vein syndrome (SwPPV) and passion fruit vine (Passiflora edulis) with bud proliferation disease (PasFBP) in the same region. Sequence analysis of cloned 16S rRNA gene sequences (GenBank accession nos FJ226068–FJ226073 and HQ225624–HQ225635) indicated that all three affected plants were infected by phytoplasmas that shared <97.5 % sequence similarity with previously described phytoplasmas. The SoyST-causing phytoplasma represents a new taxon, most closely related to phytoplasma group 16SrI and 16SrXII strains. Virtual RFLP analysis indicated that the SoyST-causing phytoplasma and its closely related strains represent a novel 16Sr group, designated 16SrXXXI. Phylogenetic analysis of 16S rRNA gene sequences from the new phytoplasma strains, those previously described as ‘Candidatus Phytoplasma spp.’ and other distinct, as yet unnamed, phytoplasmas indicated that the SoyST-causing phytoplasma represents a distinct lineage within the aster yellows/stolbur branch on the phylogenetic tree. On the basis of its unique 16S rRNA gene sequence and biological properties, strain SoyST1c1 represents a novel taxon, for which the name ‘Candidatus Phytoplasma costaricanum’ is proposed with SoyST1c1 as the reference strain.
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Aquisphaera giovannonii gen. nov., sp. nov., a planctomycete isolated from a freshwater aquarium
As part of a study of the diversity of planctomycetes, two novel strains, designated OJF2T and OJF8, were isolated from the sediments of a freshwater aquarium. The organisms were chemoheterotrophic, spherical and pink-pigmented, had an optimum growth temperature of about 30–35 °C and an optimum pH for growth of around 7.5–8.5. The predominant fatty acids were C18 : 1ω9c and C16 : 0. The two strains were able to assimilate several sugars and organic acids. 16S rRNA gene sequence analysis confirmed the affiliation of these organisms to the phylum ‘Planctomycetes’; they showed highest similarity to the type strains of Singulisphaera acidiphila (92.4 %) and Isosphaera pallida (91.9 %). On the basis of physiological, biochemical and chemotaxonomic characteristics, strains OJF2T and OJF8 are considered to represent a novel species of a new genus of the order Planctomycetales, for which the name Aquisphaera giovannonii gen. nov., sp. nov. is proposed. The type strain of Aquisphaera giovannonii is OJF2T ( = CECT 7510T = DSM 22561T).
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Morphology and molecular evaluation of Iphinoe spelaeobios gen. nov., sp. nov. and Loriellopsis cavernicola gen. nov., sp. nov., two stigonematalean cyanobacteria from Greek and Spanish caves
More LessCaves have generally been found to host phototrophic micro-organisms from various taxonomic groups, with cyanobacteria comprising an important group that have adapted to these stable and highly specific environments. A polyphasic study based on aspects of classical morphology and molecular data revealed two new monospecific genera from fresh material of Greek and Spanish caves. Both taxa are characterized by obligatory true branching (T-type, V-type and false branching), the presence of heterocysts, and reproduction by hormocysts and akinetes. They shared some similarities in their morphological characteristics as revealed by light, scanning electron and transmission electron microscopy, but phylogenetic analysis based on 16S rRNA gene sequences showed that the two phylotypes were different (89.8 % similarity); this represents an example of shared morphology in genetically different strains of cave-adapted species. Phenotypic and genetic traits strongly support classification of the phylotypes as independent taxa in the order Stigonematales (the most differentiated and complicated group of cyanobacteria), family Loriellaceae Geitl 1925. Hence, the names Iphinoe spelaeobios Lamprinou and Pantazidou gen. nov., sp. nov. and Loriellopsis cavernicola Hernández-Mariné and Canals gen. nov., sp. nov. are proposed.
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- Archaea
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Pyrococcus yayanosii sp. nov., an obligate piezophilic hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent
An obligate piezophilic anaerobic hyperthermophilic archaeon, designated strain CH1T, was isolated from a hydrothermal vent site named ‘Ashadze’, which is located on the Mid-Atlantic Ridge at a depth of 4100 m. Enrichment and isolation of the strain were carried out at 95 °C under a hydrostatic pressure of 42 MPa. Cells of strain CH1T were highly motile irregular cocci with a diameter of ~1–1.5 µm. Growth was recorded at 80–108 °C (optimum 98 °C) and at pressures of 20–120 MPa (optimum 52 MPa). No growth was observed under atmospheric pressures at 60–110 °C. Growth was observed at pH 6.0–9.5 (optimum 7.5–8.0) and in 2.5–5.5 % (w/v) NaCl (optimum 3.5 %). Strain CH1T was strictly anaerobic and grew on complex proteinaceous substrates, such as yeast extract, Peptone, and casein, as well as on sucrose, starch, chitin, pyruvate, acetate and glycerol without electron acceptors. The G+C content of the genomic DNA was 49.0±0.5 mol%. Analysis of 16S rRNA gene sequences revealed that strain CH1T belongs to the genus Pyrococcus. Based on its physiological properties and similarity levels between ribosomal proteins, strain CH1T represents a novel species, for which the name Pyrococcus yayanosii sp. nov. is proposed. The type strain is CH1T ( = JCM 16557). This strain is also available by request from the Souchothèque de Bretagne (catalogue LMBE) culture collection (collection no. 3310).
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Methanobacterium movens sp. nov. and Methanobacterium flexile sp. nov., isolated from lake sediment
More LessTwo mesophilic methanogenic strains, designated TS-2T and GHT, were isolated from sediments of Tuosu lake and Gahai lake, respectively, in the Qaidam basin, Qinghai province, China. Cells of both isolates were rods (about 0.3–0.5×2–5 µm) with blunt rounded ends and Gram-staining-positive. Strain TS-2T was motile with one or two polar flagella and used only H2/CO2 for growth and methanogenesis. Strain GHT was non-motile, used both H2/CO2 and formate and displayed a variable cell arrangement depending on the substrate: long chains when growing in formate (50 mM) or under high pressure H2 and single cells under low pressure H2. Phylogenetic analysis based on 16S rRNA gene sequences placed the two isolates in the genus Methanobacterium. Strain TS-2T was most closely related to Methanobacterium alcaliphilum NBRC 105226T (96 % 16S rRNA gene sequence similarity). Phylogenetic analysis based on the alpha subunit of methyl-coenzyme M reductase also supported the affiliation of the two isolates with the genus Methanobacterium. DNA–DNA relatedness between the isolates and M. alcaliphilum DSM 3387T was 39–53 %. Hence we propose two novel species, Methanobacterium movens sp. nov. (type strain TS-2T = AS 1.5093T = JCM 15415T) and Methanobacterium flexile sp. nov. (type strain GHT = AS 1.5092T = JCM 15416T).
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- Firmicutes and Related Organisms
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Virgibacillus alimentarius sp. nov., isolated from a traditional Korean food
More LessA novel, Gram-positive, rod-shaped, motile, endospore-forming, halophilic bacterial strain, J18T, was isolated from a traditional salt-fermented seafood made of gizzard shad in Korea. Colonies were convex, cream-coloured and 1.0–2.0 mm in diameter after incubation for 3 days on marine agar. Growth occurred at pH 7.0–11.0 (optimum, pH 10.0), at 4–40 °C (optimum, 37 °C) and in the presence of 0–30 % NaCl (optimum, 9–10 %). On the basis of 16S rRNA gene sequence analysis, strain J18T was related most closely to Virgibacillus byunsanensis ISL-24T (96.3 % similarity), Virgibacillus carmonensis LMG 20964T (96.2 %), Virgibacillus halodenitrificans DSM 10037T (96.0 %), Virgibacillus arcticus Hal 1T (95.5 %) and Virgibacillus necropolis LMG 19488T (95.5 %). The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content of strain J18T was 37.0 mol%. The cell-wall peptidoglycan was of the meso-diaminopimelic acid type. The major quinone was menaquinone 7 (MK-7). Based on phenotypic, chemotaxonomic and phylogenetic data, strain J18T is considered to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus alimentarius sp. nov. is proposed. The type strain is J18T ( = KACC 14624T = JCM 16994T).
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Bacillus hemicentroti sp. nov., a moderate halophile isolated from a sea urchin
A novel Gram-staining-positive, moderately halophilic, facultatively alkaliphilic, non-motile, catalase-positive, oxidase-negative, endospore-forming, facultatively anaerobic rod, designated JSM 076093T, was isolated from a sea urchin (Hemicentrotus pulcherrimus) collected from Naozhou Island in the South China Sea. Growth occurred with 0.5–25 % (w/v) NaCl (optimum 5–8 %) and at pH 6.0–10.5 (optimum pH 8.0) and 5–40 °C (optimum 30–35 °C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C14 : 0. The genomic DNA G+C content was 38.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076093T belonged to the genus Bacillus and was related most closely to Bacillus hwajinpoensis SW-72T (99.1 % 16S rRNA gene sequence similarity) and Bacillus algicola KMM 3737T (97.3 %). The combination of results from the phylogenetic analysis, DNA–DNA hybridization and phenotypic and chemotaxonomic characterization supported the conclusion that strain JSM 076093T represents a novel species of the genus Bacillus, for which the name Bacillus hemicentroti sp. nov. is proposed, with JSM 076093T ( = DSM 23007T = KCTC 13710T) as the type strain.
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Reclassification of Paralactobacillus selangorensis Leisner et al. 2000 as Lactobacillus selangorensis comb. nov.
More LessThe taxonomic status of Paralactobacillus selangorensis is described and, based on evidence presented, transfer of the species to the genus Lactobacillus with the name Lactobacillus selangorensis comb. nov. is proposed. This reclassification is supported by multilocus sequence analysis of the 16S rRNA gene and portions of the cpn60, pheS and rpoA genes. Mode of cell division and existing phenotypic information also show that P. selangorensis cannot be differentiated from the genus Lactobacillus. The type strain of Lactobacillus selangorensis comb. nov. is ATCC BAA-66T ( = LMG 17710T = CIP 106482T).
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Alkalibacterium subtropicum sp. nov., a slightly halophilic and alkaliphilic marine lactic acid bacterium isolated from decaying marine algae
Two novel strains of marine lactic acid bacteria, isolated from decaying marine algae collected from a subtropical area of Japan, are described. The isolates, designated O24-2T and O25-2, were Gram-positive, non-sporulating and non-motile. They lacked catalase and quinones. Under anaerobic cultivation conditions, lactate was produced from glucose with the production of formate, acetate and ethanol in a molar ratio of approximately 2 : 1 : 1. Under aerobic cultivation conditions, acetate and lactate were produced from carbohydrates and related compounds. The isolates were slightly halophilic, highly halotolerant and alkaliphilic. They were able to grow in 0–17.0 % (w/v) NaCl, with optimum growth of strains O24-2T and O25-2 at 1.0–3.0 and 1.0–2.0 % (w/v) NaCl, respectively. Growth of strain O24-2T was observed at pH 7.5–9.5, with optimum growth at pH 8.0–8.5. Comparative 16S rRNA gene sequence analysis revealed that the isolates occupied a phylogenetic position within the genus Alkalibacterium, showing highest similarity (99.6 %) to Alkalibacterium putridalgicola T129-2-1T. Although sequence similarity was high, the DNA–DNA relatedness value between strain O24-2T and A. putridalgicola T129-2-1T was 27 %, indicating that they are members of distinct species. The DNA G+C contents of O24-2T and O25-2 were 43.7 and 44.4 mol%, respectively, and DNA–DNA relatedness between the isolates was 89 %. The cell-wall peptidoglycan was type A4β, Orn-d-Asp. The major cellular fatty acid components were C14 : 0, C16 : 0 and C16 : 1ω9c. Based on phenotypic characteristics and genetic distinctiveness, the isolates were classified as representatives of a novel species within the genus Alkalibacterium, for which the name Alkalibacterium subtropicum sp. nov. is proposed; the type strain is O24-2T ( = DSM 23664T = NBRC 107172T).
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- Bacteroidetes
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Algoriphagus faecimaris sp. nov., isolated from coastal sediment
More LessA Gram-negative, non-motile, non-sporulating bacterial strain, designated LYX05T, was isolated from coastal sediment of Qingdao, China, on the coast of the Yellow Sea. Strain LYX05T was aerobic and heterotrophic. The strain grew optimally at 37 °C and pH 7.5 and in the presence of 2 % (w/v) NaCl. Colonies were 1–2 mm in diameter, circular, reddish orange and shiny with entire edges on marine agar medium. Cells were rods (0.3–0.5 µm wide and 0.8–1.6 µm long). The dominant fatty acids were iso-C15 : 0 (40.82 %) and C16 : 0 (10.45 %). The DNA G+C content was 42.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LYX05T was phylogenetically related to the members of the genus Algoriphagus and the closest relative was Algoriphagus hitonicola 7-UAHT (95.8 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain LYX05T was considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus faecimaris sp. nov. is proposed. The type strain is LYX05T ( = JCM 16561T = DSM 23095T = LMG 25474T).
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Pedobacter rhizosphaerae sp. nov. and Pedobacter soli sp. nov., isolated from rhizosphere soil of Chinese cabbage (Brassica campestris)
More LessTwo bacterial strains, 01-96T and 15-51T, isolated from rhizosphere soil of Chinese cabbage (Brassica campestris) were characterized by using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 01-96T was phylogenetically related most closely to Pedobacter suwonensis 15-52T and Pedobacter roseus CL-GP80T (96.7 and 96.7 % similarity, respectively), and strain 15-51T to Pedobacter borealis G-1T and P. suwonensis 15-52T (97.2 and 97.1 %, respectively). However, levels of DNA–DNA relatedness between strain 15-51T and P. borealis KACC 14287T and P. suwonensis KACC 11317T were low (<50 %). The G+C content of the genomic DNA was 37.8 mol% for strain 01-96T and 38.6 mol% for strain 15-51T. The major fatty acids of the two strains were iso-C17 : 0 3-OH, iso-C15 : 0 and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). The results of DNA–DNA hybridization and phenotypic data showed that strains 01-96T and 15-51T could be distinguished from their closest phylogenetic relatives, and that these strains represented two novel species of the genus Pedobacter, for which the names Pedobacter rhizosphaerae sp. nov. (type strain 01-96T = KACC 14938T = NBRC 107690T) and Pedobacter soli sp. nov. (type strain 15-51T = KACC 14939T = NBRC 107691T) are proposed.
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Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm
More LessThree novel aerobic, elliptic bacteria, designated DQHS4T, DQHS8 and DQHS15, were isolated from sediment of a seashore pond for sea cucumber culture in Jimo, Qingdao, on the east coast of China. Cells were Gram-, oxidase- and catalase-negative. All three strains grew at 15–42 °C, pH 5–9 and NaCl concentrations between 0.5 and 10 %. DNA–DNA hybridization experiments revealed high (>85 %) relatedness among the three novel isolates and suggested that the strains constitute a single species. Comparative 16S rRNA gene sequence analysis indicated that these bacteria had less than 90 % similarity to all described species of the phylum Bacteroidetes; the closest relative of the three isolates was Prolixibacter bellariivorans F2T, sharing only 89.6 % sequence similarity. The major cellular fatty acids were iso-C17 : 0 3-OH (19.8–20.0 %), iso-C15 : 0 (16.9–17.3 %), anteiso-C17 : 1 B and/or iso-C17 : 1 I (7.4–8.7 %), C17 : 0 2-OH (8.4 %), anteiso-C15 : 0 (8.2–8.6 %) and C17 : 1ω6c (5.6–6.0 %). The major respiratory quinone was menaquinone-7 (MK-7) and the DNA G+C content was 41.8–43.5 mol%. Based on the distinct phylogenetic position and the combination of genotypic, phenotypic and chemotaxonomic characteristics, these three strains were considered to represent a novel species of a new genus in the phylum Bacteroidetes, for which the name Sunxiuqinia elliptica gen. nov., sp. nov. is proposed. The type strain of Sunxiuqinia elliptica is DQHS4T ( = CGMCC 1.9156T = NCCB 100301T = LMG 25367T).
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- Eukaryotic Micro-organisms
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Cryptococcus yokohamensis sp. nov., a basidiomycetous yeast isolated from trees and a Queensland koala kept in a Japanese zoological park
Three strains were isolated from the nostrils of a koala and the surrounding environment in a Japanese zoological park. Sequence analysis of the nuclear rDNA internal transcribed spacer (ITS) region and the large subunit rDNA D1/D2 domains in addition to physiological and morphological studies indicated that the isolates represent a single novel species belonging to the basidiomycetous genus Cryptococcus (Tremellales, Tremellomycetes, Agaricomycotina). Phylogenetic analysis based on D1/D2 and ITS regions revealed that the novel species belongs to the Fuciformis clade. The name Cryptococcus yokohamensis sp. nov. is proposed to accommodate these isolates with strain JCM 16989T ( = TIMM 10001T = CBS 11776T = DSM 23671T) as the type strain.
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Saturnispora quitensis sp. nov., a yeast species isolated from the Maquipucuna cloud forest reserve in Ecuador
A single strain, CLQCA-10-114T, representing a novel yeast species belonging to the genus Saturnispora was isolated from the fruit of an unidentified species of bramble (Rubus sp.), collected from the Maquipucuna cloud forest reserve, near Quito, in Ecuador. Sequence analyses of the D1/D2 domains of the large-subunit rRNA gene and ribosomal internal transcribed spacer region indicated that the novel species is most closely related to the recently described species Saturnispora gosingensis, isolated from the fruiting body of a mushroom collected in Taiwan, and Saturnispora hagleri, a Drosophila-associated yeast found in Brazil. The name Saturnispora quitensis sp. nov. is proposed to accommodate this strain; the type strain is CLQCA-10-114T ( = CBS 12184T = NCYC 3744T).
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Aspergillus saccharolyticus sp. nov., a black Aspergillus species isolated in Denmark
A novel species, Aspergillus saccharolyticus sp. nov., belonging to the Aspergillus section Nigri group is described. This species was isolated in Denmark from treated hardwood. Its taxonomic status was determined using a polyphasic taxonomic approach including phenotypic (morphology and extrolite profiles) and molecular (β-tubulin, internal transcribed spacer and calmodulin gene sequences, and universally primed PCR fingerprinting) analysis. Phenotypic and molecular data enabled this novel species to be clearly distinguished from other black aspergilli. A. saccharolyticus is a uniseriate Aspergillus species that is morphologically similar to Aspergillus japonicus and Aspergillus aculeatus, but has a totally different extrolite profile compared to any known Aspergillus species. The type strain of A. saccharolyticus sp. nov. is CBS 127449T ( = IBT 28509T).
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Zygosaccharomyces gambellarensis sp. nov., an ascosporogenous yeast isolated from an Italian ‘passito’ style wine
More LessYeast strains were isolated from Vin Santo of Gambellara, a sweet white wine with the specificity of Controlled Designation of Origin produced from off-vine overripened grapes in the Veneto region (Italy). Comparative sequence analysis of the 26S rRNA gene revealed that three representative strains (ZO03-5T, CA06-8 and ME06-9) constitute a taxon related to, but distinct from, Zygosaccharomyces machadoi. Similarity between the 26S rRNA gene domain D1/D2 sequence of the three isolates and Z. machadoi was 97.9 %; moreover, the morphological characteristics and the physiological behaviour also supported recognition of a novel taxon of osmophilic non-psychrophilic yeast showing a flower-like arrangement of budding cells that remain attached to each other. The name Zygosaccharomyces gambellarensis is proposed for the novel species, with ZO03-5T ( = CBS 12191T = MUCL 53393T) as the type strain.
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- Evolution, Phylogeny and Biodiversity
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Evolution and variation of the nifD and hupL elements in the heterocystous cyanobacteria
More LessIn heterocystous cyanobacteria, heterocyst differentiation is accompanied by developmentally regulated DNA rearrangements that occur within the nifD and hupL genes, referred to as the nifD and hupL elements. These elements are segments of DNA that are embedded within the coding region of each gene and range from 4 to 24 kb in length. The nifD and hupL elements are independently excised from the genome during the later stages of differentiation by the site-specific recombinases, XisA and XisC, respectively, which are encoded within the elements themselves. Here we examine the variation and evolution of the nifD and hupL elements by comparing full-length nifD and hupL element sequences and by phylogenetic analysis of xisA and xisC gene sequences. There is considerable variation in the size and composition of the nifD and hupL elements, however, conserved regions are also present within representatives of each element. The data suggest that the nifD and hupL elements have undergone a complex pattern of insertions, deletions, translocations and sequence divergence over the course of evolution, but that conserved regions remain.
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A multilocus sequence analysis approach to the phylogeny and taxonomy of the Halobacteriales
Members of the order Halobacteriales are obligate extreme halophiles that belong to the domain Archaea. The classification of the Halobacteriales currently relies on a polyphasic approach, which integrates phenotypic, genotypic and chemotaxonomic characterization. However, the most utilized genetic marker for phylogeny, the 16S rRNA gene, has multiple drawbacks for use with the Halobacteriales: the species of many genera exhibit large intragenic differences between multiple ribosomal RNA operons, the gene is too conserved to discriminate reliably at the species level and it appears to be the most frequently recombined gene between closely related species. Moreover, the Halobacteriales is a rapidly expanding group due to recent successes at cultivating novel strains from a diverse set of hypersaline environments; a fast, reliable, inexpensive, portable molecular method for discriminating species is required for their investigation. Recently, multilocus sequence analysis (MLSA) has been shown to be an effective tool for strain identification and taxonomic designation, even for those taxa that experience frequent lateral gene transfer and homologous recombination. In this study, MLSA was utilized for evolutionary and taxonomic investigation of the Halobacteriales. Efficacy of the MLSA approach was tested across a hierarchical gradient using 52 halobacterial strains, representing 33 species (including names without standing in nomenclature) and 14 genera. A subset of 21 strains from the genus Haloarcula was analysed separately to test the sensitivity and relevance of the MLSA approach among closely related strains and species. The results demonstrated that MLSA differentiated individual strains, reliably grouped strains into species and species into genera and identified potential novel species and also family-like relationships. This study demonstrates that MLSA is a rapid and informative molecular method that will probably accommodate strain analysis at any taxonomic level within the Halobacteriales.
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Phenotypic variability and phylogenetic relationships of the genera Tolypothrix and Calothrix (Nostocales, Cyanobacteria) from running water
More LessThe taxonomy of heterocystous cyanobacteria belonging to the genera Calothrix and Tolypothrix has long been a matter of debate, but their phylogenetic relationships are still not well understood. Our aim was to compare the phylogeny and morphology of members of these genera, which exhibit basal–apical polarity. A phylogeny was reconstructed on the basis of 16S rRNA gene sequences and compared with the morphological characterization of new isolates and environmental samples. Strains isolated from several rivers and streams showed a high degree of tapering when they were cultured in a nutrient-rich medium. However, clear differences were apparent when they were transferred to a nutrient-poor medium. Some strains showed a low degree of tapering and other morphological features corresponding to the genus Tolypothrix, such as false branching, whereas others maintained the morphological characteristics of the genus Calothrix. Phylogenetic analysis was congruent with the phenotypic characterization, in which the strains and environmental samples of the Tolypothrix and Calothrix morphotypes could be clearly separated. Isolates with a low degree of tapering and natural samples of Tolypothrix distorta were grouped in the same cluster, but strains of the genus Calothrix fell into well separated clades. Results from this study showed that representatives of the genus Tolypothrix share most morphological and developmental properties and a high degree of 16S rRNA gene sequence similarity. However, although similar and sometimes overlapping morphologies may occur in isolates of the genus Calothrix, these morphotypes may be distinguished on the basis of their clear genetic divergence.
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Phylogeny of nodulation and nitrogen-fixation genes in Bradyrhizobium: supporting evidence for the theory of monophyletic origin, and spread and maintenance by both horizontal and vertical transfer
More LessBacteria belonging to the genus Bradyrhizobium are capable of establishing symbiotic relationships with a broad range of plants belonging to the three subfamilies of the family Leguminosae ( = Fabaceae), with the formation of specialized structures on the roots called nodules, where fixation of atmospheric nitrogen takes place. Symbiosis is under the control of finely tuned expression of common and host-specific nodulation genes and also of genes related to the assembly and activity of the nitrogenase, which, in Bradyrhizobium strains investigated so far, are clustered in a symbiotic island. Information about the diversity of these genes is essential to improve our current poor understanding of their origin, spread and maintenance and, in this study, we provide information on 40 Bradyrhizobium strains, mostly of tropical origin. For the nodulation trait, common (nodA), Bradyrhizobium-specific (nodY/K) and host-specific (nodZ) nodulation genes were studied, whereas for fixation ability, the diversity of nifH was investigated. In general, clustering of strains in all nod and nifH trees was similar and the Bradyrhizobium group could be clearly separated from other rhizobial genera. However, the congruence of nod and nif genes with ribosomal and housekeeping genes was low. nodA and nodY/K were not detected in three strains by amplification or hybridization with probes using Bradyrhizobium japonicum and Bradyrhizobium elkanii type strains, indicating the high diversity of these genes or that strains other than photosynthetic Bradyrhizobium must have alternative mechanisms to initiate the process of nodulation. For a large group of strains, the high diversity of nod genes (with an emphasis on nodZ), the low relationship between nod genes and the host legume, and some evidence of horizontal gene transfer might indicate strategies to increase host range. On the other hand, in a group of five symbionts of Acacia mearnsii, the high congruence between nod and ribosomal/housekeeping genes, in addition to shorter nodY/K sequences and the absence of nodZ, highlights a co-evolution process. Additionally, in a group of B. japonicum strains that were symbionts of soybean, vertical transfer seemed to represent the main genetic event. In conclusion, clustering of nodA and nifH gives additional support to the theory of monophyletic origin of the symbiotic genes in Bradyrhizobium and, in addition to the analysis of nodY/K and nodZ, indicates spread and maintenance of nod and nif genes through both vertical and horizontal transmission, apparently with the dominance of one or other of these events in some groups of strains.
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- International Committee on Systematics of Prokaryotes
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- Minutes
Volumes and issues
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Volume 74 (2024)
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